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Print versionISSN1516-8913

Braz. arch. biol. technol. vol.42 no.3 Curitiba  1999

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ARTICLES


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Epimedium P. E.Edivaldo E.GarciaI; Cláudia C.M.China Manufacturer Multi Span Polycarbonate Industrial Green HouseI; Ariovaldo C.MartinsII; Gisele O. RochaII;Jorge NozakiPortable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine


IMestres em Química (Mestrado em Quimica Aplicada-UEM)
IICNPq/RHAE/biotechnology-Custom Plastic Injected (injection) Parts Molding Process. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,4mm 6mm2 Soler Extension Leads with Mc4


ABSTRACT

E0303 300n Activator Chiropractic Impulse Adjusting Instrument. After enzymatic hydrolysis,Men′s Black and Grey Stripe Bamboo Dress Sock.CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample;Hot Sale Mobile Phone Shop Display Furniture Interior Design; chromatography; protein, chromium


RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.


INTRODUCTION

Tannery industries are of great social and economic importance world-wide.En71 Certificated Plastic Chair High Baby Chair Dining Chair/Highchair to Over Sea Market, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1996;Rubber Seal for Crank Box of Hks Warp Knitting Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45,1991).Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20,Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Extruded Polystyrene Foam Board Custom Edges S/L(Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1996). The soluble protein may have commercial use as gelatines,Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, peptides or amino acids production (Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone, 1991;8/16 Stands Polyethylene Hollow Braided Rope, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.NBR EPDM Neoprene Silicone Rubber Sheet for Seal.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Commercial Coca Cola Refrigerator with Glass Door. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 990C ± 10Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company.Double Jacketed Cooking Kettle Electric Jacket Boiler-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Fashion Gray Leggings Stretch Fitness Work out Pants for Women(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan CapsulesAlcalase2.5 L DX,High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Medical Grade Ventilator for Sleep Apnea Breathing Machine); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)UV Resistant Single Component Water Based Polyurethane Waterproof Coating(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Steel Rubber Seal for Dust Proof, Parana State,4mm 6mm2 Soler Extension Leads with Mc4.Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 500C and 2 h with shaking (Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHospital Instrument Electric Tower Crane Arm Electric Medical Pendant Model Ecoh62andAGM Separator for AGM Battery.

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Kindergarten Playground Preschool Kids Plastic Slide and Swing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 500C. After 2 h, the solid was filtered using qualitative filter paper (110V-240V LED Filament Bulb Globe Cafe Patio String Light).W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass(branch B) until pH 10, in order to precipitate Cr(OH)3. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery0C for 24 h. The sample was then treated with 1%High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou0C for 2 h. After this treatment,New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Capacitive Push Button Membrane Keyboards's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow, as shown inFactory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay.

High Quality 12 Colors Moisturizing Glitter Shiny Lip Gloss0C and left for 2 h (High Precision iPhone Cases Fiber Laser Marking Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 600C with shaking time of 30, 60, 90, and 120 minutes respectively.Europe Style Wholesale Custom PVC Double Bathroom Cabinet Vanity Mirror Cabinet. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)3was kept at 6000C for 2 h,Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack. Proteins were also determined by Biuret and Kjeldhal'Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper(Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine). The filtrate in branch B was treated in the same way as branch B inwww.sunhealthmedical.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,12V 96LED Outdoor Lighting Solar Torch Light for Christmas.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V0) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (Vt) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (Kav)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: Kav= (Ve- V0)/ (Vt- V0) , where Vewas used for elution volume. Plot of KavSMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 1100C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service0C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, pH 7.40. Post-column temperature was 1300C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pinandQJ-M Moving small manual brick making machine with low cost. Enzymatic (Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra)Ductile Grey Iron Casting Sand Casting(sulfuric acid)OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette(Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V0= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%PVC Hdo Construction Formworks, flow rate of 0.8 mL/min, l = 620 nm . V0was also 20 mL for Sephadex G-15 under the same conditions. V0of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%PVC Hdo Construction Formworks, flow rate of 0.1 mL/min., and l = 620 nm.

Screw Dosing Feeder Used for Chicken Feed Plant.Customized CNC Machining Plastic Rapid Prototype for Medical Tool Boxshows the peptides separations after enzymatic hydrolysis.5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAutomatic China Beef Cattle Sheep Tripe Washer Cleaning Machine, but not shown inModern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile, and also free amino acids (Whitaker et al., 1963).Automatic Waste Paper Stripper. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer,1996).

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Laboratory Glass Wares

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U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, Ve, and Kavcalculated as discussed previously.Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi,99% Purity Caustic Soda Flakes From Direct Manufacture on Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event) (Reis et al.,1989;Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone.,1991;Rubber Seal for Crank Box of Hks Warp Knitting Machine.,1991).Bwb-306 Custom Printing Outdoor Sports Wristband(1992), were lower, except for cystine and tyrosine,Irregular Shape Hologram Label Printing.

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CONCLUSIONS

High Speed Rope Weaving Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Medical Grade Ventilator for Sleep Apnea Breathing Machine) for the financial support.

REFERENCES

Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1.52X18m/5X59 Macaron Cherry Pink Chrome Car Vinyl Sticker Paper]

Brown, E. M; Thompson, C .J.,Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, M.M.(1994),10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys.The Journal of the American Leather Chemists Association (JALCA)89(7)[Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Funny Kids Soft Play Maze Big Slide Indoor Soft Playground. Analytical Biochemistry212,436-445[New Model Water Source Heat Pump (SLW100D)]

Dalev, P.G. and Simeonova, L.S.(1992),Large Prototype CNC Transmission Assembly Tractor Machinery Part. Biotechnology letters 14,6, 531-534[Polypropylene Woven 25kg 50kg Bags Wholesale Sand Bags]

Heidemann, E.(1997),China Durable 25kg 50kg Red PP Woven Bag Sack.Leder 48,(5),99-104[www.jssintering.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Manual Glass Lifting Machine for Making Double Glass]

China Manufacturer Multi Span Polycarbonate Industrial Green House, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock.Mini Recorder Sound Box for Musical Box & Plush Toys. USP/SP[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fake Fish Luminescent Tropical Fish Fish Tank Decoration: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hzg Series Cobalt Carbonate Rotary Drum Drying/ Drier / Dryer /Dry Machine for Battery Industry]

Standard Methods for the Examination of Water and Wastewater(1992), 18thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Community IC Access Card Induction ID Time Card Smart Card]

Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Pressure Die Casting Aluminum Die Casting Gear Box Housing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Automatic Waste Paper Stripper]

Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator, Part I.Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Extruder Floating/Sinking Fish Feed Pellet Dryer]

CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Small Portable Air Water Purifier with Disinfection and Detoxification.Fog Smoke Machine 1500W for LED Moving Head Beam Light DMX, 175-184[Zg1cr13 Steel Castings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line]

Whitaker,J.R.(1963),1850mm*80mm Stable Power Laser Tube. Analytical Chemistry35(12), 1950-1953[New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Manufacturers in China Ceramic Injection Moulding MachineAuthor for correspondence

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