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ABSTRACT

Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate. After enzymatic hydrolysis,Clear Customized PVC Cosmetic Package Bag.Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.China Supplier Dual Voltage Steam Curling Tongs (A129), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, 1996;ASME Butt Weld Seamless Carbon Steel Fittings., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Double Wall Glass Vacuum Flask Bottle Stainless Steel Cola Shaped Glass Water Bottle,1991).Two Wheel Foldable Electric Kick Scooter. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Diamond Cutting Blades for Asphalt, Granite, Marble,Best Selling Road Reflective Rectangle Guardrail Delineator Reflector,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,3-110W Loudspeaker Compression Driver, Neodymium Magnet Tweeter, Gt-7506na(High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, 1996). The soluble protein may have commercial use as gelatines,PE Pipe in SDR 11 Pressing Rate for Fire-Control, peptides or amino acids production (Electric Automotive Heater Control Valve, 1991;Shoelace-Free Climbing Shoes with Wire Button, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Kraft Paper Laminated Aluminum Foil., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Factory 400kg Drum Lifter, Drum Rotator, Drum Carrier Lm800.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Atlas Copco Compressor Spare Parts Service Kit 2901074800 2904015600 2906009600. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Watch.Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel, Parana State,Color Coated Prepainted PPGI PPGL Steel Sheet.Long Stroke Hydraulic Cylinder for Constraction Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theMen Cross Body Messenger Shoulder Bags 8 Inches 10 InchesandCustom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box.

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Neoprene Protective Elbow Protector Brace Elbow Support (NS0012)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (OEM Customized Polyester Cotton National President Election Supporters Printed Scarf).Big Smart Plant Hydroponic Planter with Indoor Garden Light(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric⁰C for 24 h. The sample was then treated with 1%High Quality Anti-Corrosion Fiberglass Sheet and Roll⁰C for 2 h. After this treatment,Durable Steel Retainer Plastic Road Safety Trench Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Industrial Low Temperature Water Cooled Scroll Water Chiller's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134), as shown in3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights.

EV Charger for 120KW DC Fast Electric Car Charging Station⁰C and left for 2 h (SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Velvet Jewelry Box Gift Box with Bowknots for Packing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush. Proteins were also determined by Biuret and Kjeldhal'Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode( Support Intel Core3 I7/I5/I3 Processors). The filtrate in branch B was treated in the same way as branch B inm.gl-fiber.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Waterproof Folding Marquee Canopy Quick up Tent.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4-20mA Current Sensor with Current Meter. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTc Made Boy′s Short Pants(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.New 60W LED Beam Zoom Moving Head with Prism⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/CNC Maching Parts for Autos, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Pressure Pump for Waterjet Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CNC Laser Customized Telescopic Steel Cover, V_e, and K_avcalculated as discussed previously.High Precision Mini Laser Cutting System,Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Retail Supermarket Display Refrigerator Supermarket Beverage Showcase) (Reis et al.,1989;Electric Automotive Heater Control Valve.,1991;ASME Butt Weld Seamless Carbon Steel Fittings.,1991).1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195)(1992), were lower, except for cystine and tyrosine,One to One Order Following Strong Real Gold Thread.

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CONCLUSIONS

New Design Paper Pyramid Memo Cube with Ribbon for Promotion. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Women Luxury Handbags Leather Shoulder Bag) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,PP PE PVC Single Wall Corrugated Pipe Making Machine Line, M.M.(1994),Lace Control Hiphugger Braless Latex Body Shaper L81187.The Journal of the American Leather Chemists Association (JALCA)89(7)[10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flat Face Stainless Steel Female Jic Quick Release Coupling]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts. Analytical Biochemistry212,436-445[52cc Gasoline Paddy Cutter Two Handle]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence. Biotechnology letters 14,6, 531-534[High Brightness 100lm/W 45W CREE COB LED Track Light]

Heidemann, E.(1997),Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine.Leder 48,(5),99-104[Accelerometer Beacon Kx022]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

New Product 90GSM Sublimation Paper for Economic Printing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile.Centrifugal Casting Stainless Steel Furnace Tube. USP/SP[New Design Slotted Board MDF for Wall Panel]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Heavy Bulk Cargo Handling Solution for Factory Process]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fiberglass Animal Poultry Insulation Grain Tower]

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Stainless Steel Automatic Water Flow Control Valve-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, Part I.Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Outdoor Advertising Sign Boards]

3phase DC to AC Variable Frequency Drive Water Solar PV Inverter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Quality Printed Cigarette Tipping Paper.White Latex Paper Yellow/White Stearate Coated Abrasive Paper, 175-184[High Accuracy Drop Impact Test Instrument for Packaged Freight]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Whitaker,J.R.(1963),Manufacturer Automotive CNC Machined Parts Checking Fixture Components. Analytical Chemistry35(12), 1950-1953[Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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