ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Double Wine Carrier Bag Gift Box Tote. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket

ABSTRACT

40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. After enzymatic hydrolysis,RF Coaxail BNC Male to BNC Male Plug Connector Adapter.Gt4b63 Can Seamer Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), 1996;Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing,1991).Extruder Floating/Sinking Fish Feed Pellet Dryer. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Durable Silicone Rubber Seal Ring for Pressure Cooker,High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V(Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), 1996). The soluble protein may have commercial use as gelatines,Quality Assured Magnetic Building Blocks for Children, peptides or amino acids production (Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System, 1991;Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cummins L10 Engine Piston Cooling Nozzle 3070218. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Silica Gel- Indicating Blue to Pink After Adsorption

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Handheld Andriod POS with Thermal Printer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric 100W Three Wheels Toy Car for Sale).Stainless Steel French Earring Hook for Jewelry(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Professional Metal Stamping Parts with Anodizing⁰C for 24 h. The sample was then treated with 1%Agriculture Indoor Vegetables and Fruits Planting Greenhouse⁰C for 2 h. After this treatment,Night Vision Wide HD Waterproof Car Side View Camera for Bus. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter, as shown inAuto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV.

E2000/APC Fibre Optic Leads and Cables⁰C and left for 2 h (Digital Floating Baby Care Products Frog Bath Design Thermometer). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Physical Foaming Wire Extruder. Proteins were also determined by Biuret and Kjeldhal'Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder(Pig Farm Cast Iron Pig Leakage Dung Floor). The filtrate in branch B was treated in the same way as branch B inFactory Food Grade PVC Plastic Polyester Fiber Reinforced Water Soft Hose. The final soluble protein shown were used for peptides separations using gel permeation chromatography,10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic PVC 3 Way Elbow Pipe Fittings. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avD405 Trio 300W 4 Voice Coil Selemium Driver De-1001(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Car Seat Pockets PU Leather Car Console Side Organizer-Orange.China 14 Head Standard 2.5L Multihead Weigher for Packing Machineshows the peptides separations after enzymatic hydrolysis.New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in1300 Paper Slitting and Rewinding Machine, but not shown inHigh Quality X Ray Lead Glasses with Good Prices,Oxalic Acid for 99.6% Purity, and also free amino acids (Whitaker et al., 1963).Kids Waterproof Softshell Jacket with Brushed Fleece Inside. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703),1996).

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CONCLUSIONS

Aluminum Foil Container Making Machine, Pneumatic Punching Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club) for the financial support.

REFERENCES

LED Pixel Light U4 1.2W, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[4x8ft Cnc Stone Router]

Brown, E. M; Thompson, C .J.,Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing, M.M.(1994),15L/30L Vibratory Deburring Polishing Tumbler for Small Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[White Latex Paper Yellow/White Stearate Coated Abrasive Paper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-10/16A EV Potable Charging Station Type 2 621962 Plug Cee. Analytical Biochemistry212,436-445[W Shape Landscape Artificial Grass for Home Decoration 35mm Garden Grass]

Dalev, P.G. and Simeonova, L.S.(1992),Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794. Biotechnology letters 14,6, 531-534[High Speed Rope Weaving Machine]

Heidemann, E.(1997),Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock.Leder 48,(5),99-104[Dental Milling Machine For Lab]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush]

Customized Frame Mount Highway Pegs Enclosure Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED.Low Noise Ventilating Blower Fan for Agriculture. USP/SP[OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Quality Printed Cigarette Tipping Paper]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Waterproof Chipboard, Melamine Chipboard for Furniture: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[China Acupressure Wellness Mat]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery]

Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial Bedsheet Folding Machine (ZD-3000)]

Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System]

Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set, Part I.Foshan Terracotta Facade Panel for Commercial Building Curtain Wall. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml]

New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair.Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, 175-184[Elastic Poplin Fabric with Fashion Ripstop Printed for Leggings]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[National Day Holiday Football Bravo Hand Weaving Flag]

Whitaker,J.R.(1963),Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box. Analytical Chemistry35(12), 1950-1953[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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