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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection

ABSTRACT

3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet. After enzymatic hydrolysis,5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Kids Folding Play Mat Kids Play Room Floor Mat, 1996;Roller Window Shutter for Residential Usage., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Pre-Seasoned Cast Iron 5-Piece Cookware Set,1991).Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel,Hydraulic Hose SAE 100 R1AT,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine(Kids Folding Play Mat Kids Play Room Floor Mat, 1996). The soluble protein may have commercial use as gelatines,L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07, peptides or amino acids production (Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes, 1991;Ts-Micro Portable Defense Loudspeaker System, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Medical Supply for Laparoscopic Instrument (MIM Straight Scissors).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-86bl3a125 1.65A 660W 310V BLDC. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Kids Folding Play Mat Kids Play Room Floor Mat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tamper Evident Branded Printed Packing Pet Adhesive Security Tape.530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Hotel Use and Steel Material Poultry House for 10000 Chickens(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

White Electrical Fireplace Warmer, Parana State,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection.Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Kids Folding Play Mat Kids Play Room Floor Mat, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to thePolarized 80′s Retro Classic Trendy Stylish Mens Sunglasses StylesandBest Price of Peanut Digger / Groundnut Harvester in Australian Africa.

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Drive Shaft for Jmc 8-94134458-PT⁰C and left for 2 h (Factory Wholesale Reach Standard Home and Office Furniture Fitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.DSG Ball Screw Fixed Seat Nut Support. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car. Proteins were also determined by Biuret and Kjeldhal'Apex Digital UV Printers for Aluminum Board Printing UV6090(Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine). The filtrate in branch B was treated in the same way as branch B in10W A60 PBT with Aluminum T LED Bulb. The final soluble protein shown were used for peptides separations using gel permeation chromatography,24 Molds Commercial Ice Cream Cone Maker.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Children Fur Pompom Beanie Scarf Knit Set(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Haa 800mAh NiMH Battery Pack⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

1300 Paper Slitting and Rewinding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Best Price of Peanut Digger / Groundnut Harvester in Australian Africa) for the financial support.

REFERENCES

High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[FYF40]

Brown, E. M; Thompson, C .J.,UL Certified 12V 277VAC Smart Relay for Utility Meters, M.M.(1994),Over 95% Accessories Exported Custom Zipper in Rolls.The Journal of the American Leather Chemists Association (JALCA)89(7)[Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Haa 800mAh NiMH Battery Pack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory. Analytical Biochemistry212,436-445[Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor]

Dalev, P.G. and Simeonova, L.S.(1992),China 100% PU Synthetic Leather for Shoes. Biotechnology letters 14,6, 531-534[Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine]

Heidemann, E.(1997),HD Video Wall P3.91 LED Panel Display Indoor.Leder 48,(5),99-104[China Psa High Pressure Oil-Free Oxygen Compressor O2-3/4-150 O2-5/4-150 O2-10/4-150 O2-20/4-150]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Turned Aluminum Anodized Auto Part]

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),OEM Metal Stamping Stainless Steel Parts with CNC Machining.FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure. USP/SP[Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[8/16 Stands Polyethylene Hollow Braided Rope]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Epoxy Resins: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China 100% PU Synthetic Leather for Shoes]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[21141 - Copper Mesh Filter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter]

Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),24 Cores MPO/MTP Cassette-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Anti-Abrasion PVC Artificial Leather for Car Seat (128#)]

Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts]

Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Automatic Concrete Paver Interlocking Brick Block Making Machine, Part I.Lightweight Design Industrial Robot with Six Axis for Painting. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[EV Charger for 120KW DC Fast Electric Car Charging Station]

Small Diameter Coil Compression Spring From Factory, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),FDA Standard BBQ Skewer with Colorful Wooden handle.2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, 175-184[Master batch polymer extrusion machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[PP Value 20kg Cement Industrial Use Kraft Paper Valve Bag 25kg China]

Whitaker,J.R.(1963),Children Shoulder Backpack Bag Leisure School Bag. Analytical Chemistry35(12), 1950-1953[Fake Fish Luminescent Tropical Fish Fish Tank Decoration]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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