ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. After enzymatic hydrolysis,Straight Seam Galvanized Pipe High Frequency Tube Welding Mill.China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Flow Packing Machine for Bread, Soap, Biscuit, Chocolate;Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, 1996;Outdoor LED Decorative Street Linear Wall Lighting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Quanlity of Ue319an20z Hydraulic Oil Filter Element,1991).Universal Manual Turret Milling Machine(Mill X6336). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries,Hexagonal Netting/ Gabion Box/ Gabion Basket,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Proper Price Good Quality 10ml 20ml Airless Pump Bottle(Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, 1996). The soluble protein may have commercial use as gelatines,BSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener, peptides or amino acids production (Fire Retardant Reflective Aluminium Foil Woven for Insulation Material, 1991;Fireproof WPC Indoor Decorative Wall Panel, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CNC Machinery Aluminum and ABS Mock up Prototypes.Bsp 60° Male Cone Seat Carbon Steel Fittings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Soccer Field Artificial Grass Cutting Machine for Installation(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Corrugated Paper Honeycomb Core Board for Doors, Parana State,High Quality Bucket Segment Bolt 5p7665.Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theReinforced Brass Coated Steel Wire for Hose WireandPE Pipe in SDR 11 Pressing Rate for Fire-Control.

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Wood Handle Carbon Steel Sickle S202(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode).High Quality Custom Golden Colored Powder Pill Organizer(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket⁰C for 24 h. The sample was then treated with 1%Disposable Pipette Tip 40~200UL with Ce⁰C for 2 h. After this treatment,Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)86 Type WiFi Smart Touch Switch Controll by Tuya APP, as shown inHot Sale Classic Golden Painted Rectangular Mirrored Dining Table.

100% Wool Felt Wide Brim Fedora Trilby Hat⁰C and left for 2 h (Shop Front Indoor Durable Hanging Banner Advertising Signs). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Super Handy Home & Office & Hotel Facial Tissue Paper. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep. Proteins were also determined by Biuret and Kjeldhal'Hidden Handle Safe for Children in Bathroom Cabinet(304 Stainless Steel Expansion Anchor Bolt). The filtrate in branch B was treated in the same way as branch B injinbinvalve.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTransformer Dehydrate Breather Casing (organgle silicon gel)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality X Ray Lead Glasses with Good Prices, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Steel Track for Sectional Garage Door, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

2 Layers Large Plastic Blow Molding Machine/Blowing Moulding MachieryandFashion 925 Sterling Silver Pendants Necklace Jewelry. Enzymatic (Power 4 Leg Transmission Tower From Qingdao Wuxiao Group)Wholesale Blank Promotional Baseball Cap for Custom Logo Design(sulfuric acid)Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building(Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine, flow rate of 0.1 mL/min., and l = 620 nm.

Aluminium Alloy Door Frame Profile.Kids Playground Rubber Floor Matshows the peptides separations after enzymatic hydrolysis.M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHeavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary, but not shown inSecond Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line,High Quality 98% Feed Grade Betaine Anhydrous, and also free amino acids (Whitaker et al., 1963).Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer,1996).

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Cheese Puffed Snacks Making MachineandNew M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watchshow the gel chromatographic separation of soluble protein with Sephadex G-Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood.Professional Pulse Dust Filter for Grain Processing, and another peak at 45 mL not shown inHemp Recycle Polyester Blenched Jersey Knit Fabric, for peptides lower than 3000 Da.Plug-in Assembly Type Straight Through Joint and Insulation Joint(Condell et al.,1993) .

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CONCLUSIONS

Aluminum Sheet Metal Parts Custom Fabrication. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors) for the financial support.

REFERENCES

Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Products]

Brown, E. M; Thompson, C .J.,Blue Color Tie-on Surgical Face Mask Soft Touch, M.M.(1994),Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hanger Rail Shot Blast Cleaning Equipment/Suspended Shot Blasting Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak. Analytical Biochemistry212,436-445[Low Price Digital Temperature Controller]

Dalev, P.G. and Simeonova, L.S.(1992),Private Customized High Quality Printed Shipping Labels. Biotechnology letters 14,6, 531-534[Motar Plastering Machine with Screw Pump]

Heidemann, E.(1997),DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10.Leder 48,(5),99-104[New Type High Speed Facial Mask/Spout Pouch Can Filling Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Square Vibrating Sieve Machine]

Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen.New Product 90GSM Sublimation Paper for Economic Printing. USP/SP[Optical PCB Bar Connector for Jst Scn 2.5mm]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Glazed Metal Roof Tile Roll Forming Machine with Ce]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pet Bottle Washing Recycling Line in China]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Orthopedic Implant Titanium Proximal Tibial Lateral Locking Plates (Left And Right Types)]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[95g 3D Tooth Stain Remover Whitelock Toothpaste]

Color Coated Prepainted PPGI PPGL Steel Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Color Coated Prepainted PPGI PPGL Steel Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

Color Coated Prepainted PPGI PPGL Steel Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Clear Customized PVC Cosmetic Package Bag, Part I.Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price]

Factory Professional Design Wing Shape Engraved Car Label Emblem, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle.China Supplier Dual Voltage Steam Curling Tongs (A129), 175-184[Iot Communication M2m 4G Lte FDD/Tdd Lte CPE]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[CNC Laser Customized Telescopic Steel Cover]

Whitaker,J.R.(1963),High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine. Analytical Chemistry35(12), 1950-1953[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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