ARTICLES

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ABSTRACT

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Key words;High Temperature & IR Drying Electronic Oven / Furnace;Ce Approved Bowrider Leisure Boat for 6-8 People; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High End Folding Customized Golden Chocolate Paper Box, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, 1996;Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Concrete Electric Pole Pile Making Spinning Machine,1991).Shop Front Indoor Durable Hanging Banner Advertising Signs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Cosmetic & Food Grade Sodium Hyaluronate,High Frequency Circular Vibrating Screen,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fiberglass/Composite Mesh for Walkway FRP Grating(HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, 1996). The soluble protein may have commercial use as gelatines,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China, peptides or amino acids production (Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, 1991;Union NPT Bsp BSPT Stainless Steel Pipe Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tear Strip Tape.Healthy Grooming Kit for Caring Newborn Baby-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

High Quality Aluminum Street Light Pole, Parana State,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines.WPC Wall Cladding Waterproof Exterior Wall Panels, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the99% Purity Caustic Soda Flakes From Direct Manufacture on Saleand150kw Plastic Bottle Recycling Equipment.

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Customized Permanent Strong Nickel Block Neodymium Magnet(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (4mm 6mm2 Soler Extension Leads with Mc4).Tall Thin Lowes Wood Storage Cabinet (GSP20-008)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)⁰C for 24 h. The sample was then treated with 1%Stainless Steel LED Underground Light, Ground Lighting IP66⁰C for 2 h. After this treatment,GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)K Series Helical Bevel Reduction Gearbox Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps, as shown inT5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPETG Acrylic Sheet for Window(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, pH 3.25., mobile phase B: buffer solution of 0.01 mol/14 Head Multihead Weigher for Pork with Screw Feeding, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products.China Supplier Laboratory Mixer for Comsticshows the peptides separations after enzymatic hydrolysis.High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inJhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2), but not shown inAdult Neoprene Sport Summer Beach Aqua Swimming Shoes,Large Jewelry Display Box for Ring Necklace Earring Storage Case, and also free amino acids (Whitaker et al., 1963).Indoor Tub Customized Shower/Free Standing Shower Enclosure. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee,1996).

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Wall Shelf

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CONCLUSIONS

Custom Black and White Hard Laptop Case Travel Suitcase. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System) for the financial support.

REFERENCES

Concrete Road Crack-Pouring Machine for Construction, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Advertising Back Light/Halo Letter Sign]

Brown, E. M; Thompson, C .J.,Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, M.M.(1994),China OEM Alloy Steel Precision Casting.The Journal of the American Leather Chemists Association (JALCA)89(7)[Durable Steel Retainer Plastic Road Safety Trench Cover]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Precision Rail Slider 20mm Diameter PMI Linear Guideway]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport. Analytical Biochemistry212,436-445[Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate]

Dalev, P.G. and Simeonova, L.S.(1992),China Best Quality Hose Crimping Machine. Biotechnology letters 14,6, 531-534[Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl]

Heidemann, E.(1997),1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor.Leder 48,(5),99-104[Semi-Automatic Pneumatic300 Pipe Cutting Machine for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ IV Fluid Set with Precision Filter & Luer Lock]

Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Galvanized Metal Welded PVC Coated Wire Mesh Fence.Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand. USP/SP[Professional Sound System Vt215 Line Array Speaker]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Wholesale Logo Printing Cheap Gold Custom Paper Clip]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-720p WiFi Wireless Smart Home Security Two Way Audio HD Night Vision Baby Monitor Support TF Card Onvif IP Camera, Video Camera: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[EPE Foam Package Extruding Production Line]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Outdoor Equitment Durable Cmouflage Snow Wear for Hunting Paintball Games]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Outdoor P5mm Programmable Bus LED Display Sign]

Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building]

Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Top Quality Sanitary Tube Shell Tube Heat Exchanger, Part I.Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)]

Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Precision iPhone Cases Fiber Laser Marking Machine.Auto Water Cooling Radiator Release Temperature Screw Air Compressor, 175-184[Erbium Fiber Amplifier]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Food Grade PE Coated Kraft Paper for Paper Cup]

Whitaker,J.R.(1963),Injection Mold Maker for Plastic Fighter Toy. Analytical Chemistry35(12), 1950-1953[Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile EarphoneAuthor for correspondence