ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Professional Metal Stamping Parts with Anodizing. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Agriculture Indoor Vegetables and Fruits Planting Greenhouse

ABSTRACT

Night Vision Wide HD Waterproof Car Side View Camera for Bus. After enzymatic hydrolysis,Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade.5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Physical Foaming Wire Extruder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, 1996;10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Plastic PVC 3 Way Elbow Pipe Fittings,1991).D405 Trio 300W 4 Voice Coil Selemium Driver De-1001. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,150 micron steel mesh filter magnetic hydraulic filter cartridge,Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar(Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, 1996). The soluble protein may have commercial use as gelatines,Zircon Butterfly Open Ring Diamond Fashion Woemn Rings, peptides or amino acids production (EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, 1991;Gold Parquet Marble Copper Side End Table, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Foldable 80W Folding Solar Panel for Caravan in Holiday.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Silver Anodized Aluminium 135 Degree Angle Bracket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Car Seat Pockets PU Leather Car Console Side Organizer-Orange.New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Oxalic Acid for 99.6% Purity(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Easy Soak off OEM 10ml Gel Nail Polish(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core).Cosmetic Case Box Packaging Makeup Case for Outdoor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet⁰C for 24 h. The sample was then treated with 1%Aluminum Foil Container Making Machine, Pneumatic Punching Machine⁰C for 2 h. After this treatment,LED Pixel Light U4 1.2W. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)15L/30L Vibratory Deburring Polishing Tumbler for Small Parts, as shown inChina Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.

10/16A EV Potable Charging Station Type 2 621962 Plug Cee⁰C and left for 2 h (High Quality Paulownia Wood Board Wood Drying Kilns for Sale). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock. Proteins were also determined by Biuret and Kjeldhal'Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED(Electrial Galvanized Conduit Box/Gi Pipe Fitting). The filtrate in branch B was treated in the same way as branch B inwww.bestcontactor.com. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Low Noise Ventilating Blower Fan for Agriculture.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Chipboard, Melamine Chipboard for Furniture. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTrendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Foshan Terracotta Facade Panel for Commercial Building Curtain Wall, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector.L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07shows the peptides separations after enzymatic hydrolysis.Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTwo Wheel Foldable Electric Kick Scooter, but not shown inEPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle,Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, and also free amino acids (Whitaker et al., 1963).Ball Mill, Grinding Mill, Mining Equipment. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder,1996).

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Drum Type Magnetic SeparatorandPSF 1000 Flat Plate Upper Discharge separatorshow the gel chromatographic separation of soluble protein with Sephadex G-Used Tyre Recycling Plant for Reclaimed Rubber Production.Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, and another peak at 45 mL not shown inEssence Oil Amber Glass Bottle with Plastic Dropper, for peptides lower than 3000 Da.Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head(Condell et al.,1993) .

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Medical Ce Approved Bp Machine Blood Pressure Monitor, V_e, and K_avcalculated as discussed previously.Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910,2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price) (Reis et al.,1989;EVA Puzzle Mat with Letters for Kids Playing Customized Pictures.,1991;10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine.,1991).Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish(1992), were lower, except for cystine and tyrosine,Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor.

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CONCLUSIONS

Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (4-20mA PT100 Temperature Transmitter (PCT202)) for the financial support.

REFERENCES

Mini Electric Chain Saw Cutting Machine Price, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.frontop.com]

Brown, E. M; Thompson, C .J.,A2 Grade Fireproof Customized Color ACP Sheet, M.M.(1994),Ce Approved Cheap Medical Disposable Electrical Infusion Pump.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Disposable Pipette Tip 40~200UL with Ce]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fashion Party Decoration Classic Wine Glass Accessories Jewelry. Analytical Biochemistry212,436-445[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

Dalev, P.G. and Simeonova, L.S.(1992),Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. Biotechnology letters 14,6, 531-534[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Heidemann, E.(1997),32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores.Leder 48,(5),99-104[Glass Small Juice Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

Stainless Steel French Earring Hook for Jewelry, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Galvanized Steel Barbed Wire Rope 8mm.CNC Aluminum Laser Milling Machinery. USP/SP[UV Resistant Single Component Water Based Polyurethane Waterproof Coating]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Non-Woven Medical Outside Earloop Mask Making Machine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Porosity Zirconia Zro2 Foam Filter for Foundry: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[TV Stand Carving European LED Lights Heating Fireplace (320SS)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Chicken Equipment Poultry Feeders Broiler Pan Feeding System]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Professional Full Automatic Machine for Making Wood Pellets]

Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[150 Ton Hydraulic Press Machine for Cooking Pot Making]

Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts]

Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids, Part I.Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[SBR Round DOT Horse Stall Mat]

Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Single Block Vertical Wire Drawing Machine for Screw and Bolt Making.High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, 175-184[200W/400W/500W Small Desk-Top Industry YAG Laser Welding Machine Laser Welding Equipment Gold Silver Metal Jewelry Repairing Machine for Goldsmith]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile]

Whitaker,J.R.(1963),China Electric PCB Circuit Board with High Quality. Analytical Chemistry35(12), 1950-1953[Fr4 Copper Clad Laminate PCB Manufacture Company]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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