ARTICLES

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ABSTRACT

Manual Electric Winch Small Winch 220V Winch. After enzymatic hydrolysis,250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, 1996;3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet,1991).5-Axis CNC Machining Billet Aluminum Engine Rotary Housing. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashionable Simple Printing Mini Key & Coin Purse(Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, 1996). The soluble protein may have commercial use as gelatines,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch, peptides or amino acids production (Kids Folding Play Mat Kids Play Room Floor Mat, 1991;Roller Window Shutter for Residential Usage, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Pre-Seasoned Cast Iron 5-Piece Cookware Set., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Hydraulic Hose SAE 100 R1AT.Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

86bl3a125 1.65A 660W 310V BLDC, Parana State,Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS).Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWaterproof ChipboardandCustom Black and White Hard Laptop Case Travel Suitcase.

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530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Manufacturer Automotive CNC Machined Parts Checking Fixture Components).Hotel Use and Steel Material Poultry House for 10000 Chickens(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch⁰C for 24 h. The sample was then treated with 1%Kontron One-Piece 10-Lead EKG Cable with Resisitance⁰C for 2 h. After this treatment,Best Price of Peanut Digger / Groundnut Harvester in Australian Africa. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Manufacture Table Type CNC Horizontal Boring Milling Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)White Electrical Fireplace Warmer, as shown inSuperior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press.

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor⁰C and left for 2 h (Custom Velvet Jewelry Box Gift Box with Bowknots for Packing). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. Proteins were also determined by Biuret and Kjeldhal'1 Inch Stainless Steel Flexible Exhaust Pipe(Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument). The filtrate in branch B was treated in the same way as branch B inPlug Gate Valve. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAps Replacement Air Compressor Parts (1627410123) Air Oil Separator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Drive Shaft for Jmc 8-94134458-PT⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DSG Ball Screw Fixed Seat Nut Support, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Haa 800mAh NiMH Battery Pack. 115V AC Single Phase Electric Motor Washing Machine Motorshows the peptides separations after enzymatic hydrolysis.Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inFlame Retardant FRP/GRP Round Pipe, but not shown in40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls,Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, and also free amino acids (Whitaker et al., 1963).Electronic Muscle Stimulator EMS Machine for Slimming. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Aluminum Plate Fin Heat Exchanger/Oil Cooler Core,1996).

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CONCLUSIONS

Australia Standard Hot Sale 750ml Glass Wine Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm) for the financial support.

REFERENCES

Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[3000W Metal Laser Cutting Machine for Stainless Steel]

Brown, E. M; Thompson, C .J.,5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, M.M.(1994),Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings.The Journal of the American Leather Chemists Association (JALCA)89(7)[Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Horizontal Milling and Boring CNC Lathe Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Square Vibrating Sieve Machine, Linear Vibrating Screen. Analytical Biochemistry212,436-445[Cog Mono Round Display LCD Module]

Dalev, P.G. and Simeonova, L.S.(1992),Manufacturers in China Ceramic Injection Moulding Machine. Biotechnology letters 14,6, 531-534[Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric]

Heidemann, E.(1997),110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine.Leder 48,(5),99-104[Basketball Jersey Fabric]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Tfl Blue Rubber Transmission Belts/Automotive Spare Part]

20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture.1300 Paper Slitting and Rewinding Machine. USP/SP[150 micron steel mesh filter magnetic hydraulic filter cartridge]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Shoelace-Free Climbing Shoes with Wire Button]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[PVC Coated Canvas PVC Tarpaulin Tarpaulin for Truck]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable]

UL Certified 12V 277VAC Smart Relay for Utility Meters, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Over 95% Accessories Exported Custom Zipper in Rolls-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Lime Dewatering Filter Press Cloth Filter]

UL Certified 12V 277VAC Smart Relay for Utility Meters,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Silica Gel- Indicating Blue to Pink After Adsorption]

UL Certified 12V 277VAC Smart Relay for Utility Meters, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory, Part I.China 100% PU Synthetic Leather for Shoes. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Electric 100W Three Wheels Toy Car for Sale]

HD Video Wall P3.91 LED Panel Display Indoor, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),OEM Metal Stamping Stainless Steel Parts with CNC Machining.FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure, 175-184[www.hdfmills.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

Whitaker,J.R.(1963),Epoxy Resins. Analytical Chemistry35(12), 1950-1953[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pig Farm Cast Iron Pig Leakage Dung FloorAuthor for correspondence