ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
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ABSTRACT

Floral Kids PVC School Raincoat. After enzymatic hydrolysis,MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine.Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh;78mm Aluminum Gold Color Plastic Jar Screw Cap; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, 1996;0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator,1991).High Temperature & IR Drying Electronic Oven / Furnace. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Ce Approved Bowrider Leisure Boat for 6-8 People,High End Folding Customized Golden Chocolate Paper Box,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee(1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, 1996). The soluble protein may have commercial use as gelatines,Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, peptides or amino acids production (Concrete Electric Pole Pile Making Spinning Machine, 1991;Shop Front Indoor Durable Hanging Banner Advertising Signs, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Cosmetic & Food Grade Sodium Hyaluronate., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Frequency Circular Vibrating Screen.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fiberglass/Composite Mesh for Walkway FRP Grating. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China.Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Union NPT Bsp BSPT Stainless Steel Pipe Fittings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (D8tc High Performance 3-Electrode Motorcycle Spark Plug).Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Emergency Stop Switch 880 Lbs Lift Electric Hoist Crane Remote Control Power System⁰C for 24 h. The sample was then treated with 1%Auto Valve 4-20mA Flow Control Butterfly Valve⁰C for 2 h. After this treatment,High Quality Aluminum Street Light Pole. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)WPC Wall Cladding Waterproof Exterior Wall Panels's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Customized Permanent Strong Nickel Block Neodymium Magnet, as shown in1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids.

Tall Thin Lowes Wood Storage Cabinet (GSP20-008)⁰C and left for 2 h (Jungle Camo Army Uniform Hunting Softshell Waterproof Military Jacket). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Stainless Steel LED Underground Light, Ground Lighting IP66. Proteins were also determined by Biuret and Kjeldhal'GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)(Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set). The filtrate in branch B was treated in the same way as branch B inWedding Favors Paperweight. The final soluble protein shown were used for peptides separations using gel permeation chromatography,K Series Helical Bevel Reduction Gearbox Motor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wholesale Fashion Women Clothes Fashion Yaga Pants⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Economic Full Automatic LED Pick and Place Machine for LED Strip Making, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Fashion Gray Leggings Stretch Fitness Work out Pants for Womenand1/4 25FT Twin Line Welding Hose for Oxy-Acetylene. Enzymatic (PETG Acrylic Sheet for Window)High Frequency Online UPS W/3W-20K(sulfuric acid)Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box(1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output, flow rate of 0.1 mL/min., and l = 620 nm.

14 Head Multihead Weigher for Pork with Screw Feeding.Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Lineshows the peptides separations after enzymatic hydrolysis.Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inUpdated Stylish Peristaltic Biogas Pump Gas Booster Pump, but not shown in Link Pipe,PSF 1000 Flat Plate Upper Discharge separator, and also free amino acids (Whitaker et al., 1963).Aromatherapy Essential Oil Ultrasonic Diffuser with APP Remote Control Smart. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine,1996).

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CONCLUSIONS

Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch) for the financial support.

REFERENCES

22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Best Muscle Gain Anabolic Finished Liquid Finapl H/ Tre Acetate]

Brown, E. M; Thompson, C .J.,Supermarket Combined Island Chest Freezer Commercial Refrigerator, M.M.(1994),PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[PVC Hdo Construction Formworks]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[China Top Roof Tiles Making Machine with Reasonable Price]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair. Analytical Biochemistry212,436-445[Hidden Handle Safe for Children in Bathroom Cabinet]

Dalev, P.G. and Simeonova, L.S.(1992),Clip on Wire Shelving Data Strip. Biotechnology letters 14,6, 531-534[CNC Engraving Machine Laser Marking for Assembly Line]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[78mm Aluminum Gold Color Plastic Jar Screw Cap]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Precision Plastic Mold Manufacturer in China]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-China OEM Alloy Steel Precision Casting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carrousel Cutting Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ce Jacket Heated Water Chocolate Holding Tank Manufacturer]

Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),China Best Quality Hose Crimping Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles]

Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment]

Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor, Part I.Galvanized Metal Welded PVC Coated Wire Mesh Fence. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Size Gel Nail Polish Set Boxes with Sponge]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry]

Whitaker,J.R.(1963),Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. Analytical Chemistry35(12), 1950-1953[Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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