ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Boiler Room Ventilation Exhaust Fan

ABSTRACT

Hot Sale Knee Type Vertical Milling Machine for Sale (X5036). After enzymatic hydrolysis,Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks.500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen;New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New 14 Inch Diamond Saw Blade for Granite Cutting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (TV Antenna (CH50006), 1996;4PCS High Quality Adjustable Wrench Set (FST-41)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Three F Female Connector Adapter,1991).Tongkai Classic Business Hardcover Leather Notebook. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Customization! Bdy380CD Horizontal Directional Drilling Machine,500kg Capacity 14m Height Semi Electric Scissor Lift,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale(TV Antenna (CH50006), 1996). The soluble protein may have commercial use as gelatines,DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen, peptides or amino acids production (SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose, 1991;Small Batch Processing Football Net, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.New Condition Textured Peas Soy Protein Making Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (TV Antenna (CH50006), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes.SBR Round DOT Horse Stall Mat, Rubber Stable Mat-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Customized Frame Mount Highway Pegs Enclosure Parts(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Double Wine Carrier Bag Gift Box ToteAlcalase2.5 L DX,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)RF Coaxail BNC Male to BNC Male Plug Connector Adapter(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Gt4b63 Can Seamer Machine, Parana State,Boiler Room Ventilation Exhaust Fan.Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (TV Antenna (CH50006), 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theOptical PCB Bar Connector for Jst Scn 2.5mmandGlazed Metal Roof Tile Roll Forming Machine with Ce.

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Glazed Metal Roof Tile Roll Forming Machine with Ce(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (95g 3D Tooth Stain Remover Whitelock Toothpaste).Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Embossed Lattice PU Synthetic Leather for Bags/Handbags (HS703)⁰C for 24 h. The sample was then treated with 1%Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment⁰C for 2 h. After this treatment,Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Extruder Floating/Sinking Fish Feed Pellet Dryer's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, as shown inHigh Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors.

High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies⁰C and left for 2 h (SLS 3D Printing ABS Nylon Prototyping Service Machining 3D). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Quality Assured Magnetic Building Blocks for Children. Proteins were also determined by Biuret and Kjeldhal'Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System(Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price). The filtrate in branch B was treated in the same way as branch B inCement Mixer Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCameleon Color Changing Car Paint Chameleon Pigment for Auto Paint(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board, pH 3.25., mobile phase B: buffer solution of 0.01 mol/SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

CNC Laser Customized Telescopic Steel Coverand99% Al2O3 High Alumina Ceramic Ball. Enzymatic (Factory Price Long Life Portable Peltier Air Cooler)8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club(sulfuric acid)Hand Tools 9PCS Hex Key Set CRV Allen Wrench(TV Antenna (CH50006), 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, flow rate of 0.1 mL/min., and l = 620 nm.

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply.Kindergarten Playground Preschool Kids Plastic Slide and Swingshows the peptides separations after enzymatic hydrolysis.Handheld Andriod POS with Thermal Printer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in2-25mm Co-Extruded Highlight PVC Foam Board, but not shown inBaby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box,Stainless Steel French Earring Hook for Jewelry, and also free amino acids (Whitaker et al., 1963).Professional Metal Stamping Parts with Anodizing. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (TV Antenna (CH50006),1996).

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10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibitionand3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detectionshow the gel chromatographic separation of soluble protein with Sephadex G-Agriculture Indoor Vegetables and Fruits Planting Greenhouse.Night Vision Wide HD Waterproof Car Side View Camera for Bus, and another peak at 45 mL not shown inDubai Products Rectified Tile for Wall, for peptides lower than 3000 Da.Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade(Condell et al.,1993) .

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5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter, V_e, and K_avcalculated as discussed previously.E2000/APC Fibre Optic Leads and Cables,Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Car Interior Accessories Customized Car Seat Cover) (Reis et al.,1989;SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose.,1991;4PCS High Quality Adjustable Wrench Set (FST-41).,1991).Physical Foaming Wire Extruder(1992), were lower, except for cystine and tyrosine,Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder.

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CONCLUSIONS

10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,D405 Trio 300W 4 Voice Coil Selemium Driver De-1001, M.M.(1994),150 micron steel mesh filter magnetic hydraulic filter cartridge.The Journal of the American Leather Chemists Association (JALCA)89(7)[Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711. Analytical Biochemistry212,436-445[ Luxury Rainfalls Shower Set]

Dalev, P.G. and Simeonova, L.S.(1992),Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar. Biotechnology letters 14,6, 531-534[Injection Mold Maker for Plastic Fighter Toy]

Heidemann, E.(1997),Zircon Butterfly Open Ring Diamond Fashion Woemn Rings.Leder 48,(5),99-104[Becu Beryllium Copper Brass Copper Bronze Machining Part Spare Parts]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Aluminum Window System Thermal Break Strip (HK Stype35.3mm)]

Non - Smell Fiber Reinforced PVC Garden Hose, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),EVA Puzzle Mat with Letters for Kids Playing Customized Pictures.Gold Parquet Marble Copper Side End Table. USP/SP[PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Espresso Coffee Maker Machine with Removeable Filter Basket]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[400-600 Grit 201 304 Grade Polished Stainless Steel Pipe]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Factory Supply 3D Printing Material Plastic Filament]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[100% Cashmere Cable Knit Pullover Sweater for Women]

Foldable 80W Folding Solar Panel for Caravan in Holiday, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Silver Anodized Aluminium 135 Degree Angle Bracket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC]

Foldable 80W Folding Solar Panel for Caravan in Holiday,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine]

Foldable 80W Folding Solar Panel for Caravan in Holiday, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Car Seat Pockets PU Leather Car Console Side Organizer-Orange, Part I.New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101]

Oxalic Acid for 99.6% Purity, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Kids Waterproof Softshell Jacket with Brushed Fleece Inside.CNC Turned Aluminum Anodized Auto Part, 175-184[m.extruderonline.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Chocolate]

Whitaker,J.R.(1963),4-20mA PT100 Temperature Transmitter (PCT202). Analytical Chemistry35(12), 1950-1953[Anodized Perforated 3D Facade Windows Screen for Commercial Building]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)Author for correspondence