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ABSTRACT

86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. After enzymatic hydrolysis,Cummins Fuel Injector Kit (3076736 3095023).Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Electric 100W Three Wheels Toy Car for Sale, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (New Design Customized 100% Cotton Home Towel Bath Towel, 1996;Toughened Railing Glass., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Delrin Sheets with Good Wear Resistance,1991).High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service,Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Gas and Water End Protective Covers (YZF-H26)(New Design Customized 100% Cotton Home Towel Bath Towel, 1996). The soluble protein may have commercial use as gelatines,400W Emergency Backup Battery Powerstation Portable Solar Generator, peptides or amino acids production (Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof, 1991;Automatic Electric Hydraulic Metal Bending Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;6082 and 6061 Aluminum CNC Machining Parts for Aircraft., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.1054 FRP Pressure Vessel for Water Softener & Water Treatment.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (New Design Customized 100% Cotton Home Towel Bath Towel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Non Woven Fabric for Shoe Lining Shoe Inner Good Quality.Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:HDPE Large Diameter Hollowness Wall Winding Pipe Production Line(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China).High quality Brass Forged Gate Valve with Casting Iron Handle(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)⁰C for 24 h. The sample was then treated with 1%High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture⁰C for 2 h. After this treatment,Cog Mono Round Display LCD Module. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)TV Stand Carving European LED Lights Heating Fireplace (320SS)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Quality Mini LED Flood Light SMD Flood Light 150W, as shown inAntique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet.

Low Price Fashion Unique Plastic Zipper⁰C and left for 2 h (99% Al2O3 High Alumina Ceramic Ball). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Silica Gel- Indicating Blue to Pink After Adsorption. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Offer High Quality Square and Round Cast Iron Manhole Cover. Proteins were also determined by Biuret and Kjeldhal'White Tight Long Sleeves off Shoulders Celebration Bandage Dress(Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat). The filtrate in branch B was treated in the same way as branch B inPortable 30L DC 12V/24V R134A Mini Car Fridge. The final soluble protein shown were used for peptides separations using gel permeation chromatography,OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoad Safety Reflective Waterproof Plastic Reflectors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Quality Gas Spring Strut Gas Shock for Wall Bed⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Industrial Bedsheet Folding Machine (ZD-3000), pH 3.25., mobile phase B: buffer solution of 0.01 mol/Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

ANSI B16.5 Stainless Steel SS316 Forging Blind Flange. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric) for the financial support.

REFERENCES

3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Chinese Pouring Cast Iron Ladle]

Brown, E. M; Thompson, C .J.,2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, M.M.(1994),Integrated Hydraulic Steering Control Units (BZZ1F SCU).The Journal of the American Leather Chemists Association (JALCA)89(7)[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Model Water Source Heat Pump (SLW100D). Analytical Biochemistry212,436-445[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

Dalev, P.G. and Simeonova, L.S.(1992),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment. Biotechnology letters 14,6, 531-534[Irregular Shape Hologram Label Printing]

Heidemann, E.(1997),Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube.Leder 48,(5),99-104[Royal Polyresin Pet Feeding Table Plastic Dog Cat Bowls]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality Fashion Ladies Backpack PU Leather Backpack]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Capacity Vibrating Screen Manufacturer: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Carbon Dioxide Gas with 50L Seamless Cylinder]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Temperature Resistance Temperature Probe Fast Response]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[New Product 90GSM Sublimation Paper for Economic Printing]

Electrochemical Polishing Tungsten Evaporation Materials, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons]

Electrochemical Polishing Tungsten Evaporation Materials,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[24 Cores MPO/MTP Cassette]

Electrochemical Polishing Tungsten Evaporation Materials, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:White UTP Cat5e UK Type Tooless RJ45 Keystone Jack, Part I.High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Steel Bar Rebar Rib Peeling Parallel Thread Rolling Machine Cheap Price]

CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Cheese Puffed Snacks Making Machine.Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration, 175-184[Computerized Engraving Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill]

Whitaker,J.R.(1963),China High Profit Automatic Pet Extruder Machine. Analytical Chemistry35(12), 1950-1953[Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Pre-Seasoned Cast Iron 5-Piece Cookware SetAuthor for correspondence