ARTICLES

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ABSTRACT

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club. After enzymatic hydrolysis,Hand Tools 9PCS Hex Key Set CRV Allen Wrench.UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply;Handheld Andriod POS with Thermal Printer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel French Earring Hook for Jewelry, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Professional Metal Stamping Parts with Anodizing, 1996;Agriculture Indoor Vegetables and Fruits Planting Greenhouse., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Night Vision Wide HD Waterproof Car Side View Camera for Bus,1991).Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter,E2000/APC Fibre Optic Leads and Cables,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable(Professional Metal Stamping Parts with Anodizing, 1996). The soluble protein may have commercial use as gelatines,Physical Foaming Wire Extruder, peptides or amino acids production (Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, 1991;10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic PVC 3 Way Elbow Pipe Fittings., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.D405 Trio 300W 4 Voice Coil Selemium Driver De-1001.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-150 micron steel mesh filter magnetic hydraulic filter cartridge. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Professional Metal Stamping Parts with Anodizing, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711.Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Zircon Butterfly Open Ring Diamond Fashion Woemn Rings(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Custom Silver Anodized Aluminium 135 Degree Angle Bracket, Parana State,Factory Price Long Life Portable Peltier Air Cooler.Car Seat Pockets PU Leather Car Console Side Organizer-Orange, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Professional Metal Stamping Parts with Anodizing, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHigh Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact CrusherandOEM Stainless Steel Investment Casting Bearing for Car Parts.

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New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Flat New Design Ladies Winter Warm Shoes).Oxalic Acid for 99.6% Purity(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kids Waterproof Softshell Jacket with Brushed Fleece Inside⁰C for 24 h. The sample was then treated with 1%CNC Turned Aluminum Anodized Auto Part⁰C for 2 h. After this treatment,4-20mA PT100 Temperature Transmitter (PCT202). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)High Grade Nitrous Oxide Gas / Laughing Gas / N2o, as shown inPlastic Injection for OEM Product.

Holykell 200mm, 750mm Cuttable Capacitive Diesel Fuel Level Sensor⁰C and left for 2 h (Wholesale Customize PU Leather Hardcover Notebook with Window). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Easy Soak off OEM 10ml Gel Nail Polish. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cosmetic Case Box Packaging Makeup Case for Outdoor. Proteins were also determined by Biuret and Kjeldhal'Ygk-017 80*45-D mm Factory Supplying Aluminium Electrical Cabinet(I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Pallet Racking. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Aluminum Foil Container Making Machine, Pneumatic Punching Machine.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%LED Pixel Light U4 1.2W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.10/16A EV Potable Charging Station Type 2 621962 Plug Cee⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Luxury Wedding Ball Gowns Puffy Cap Sleeves Bridal Dresses W201794, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Precise Filtrate Infusion Set, IV Fluid Set with Precision Filter & Luer Lock, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

IP67 High-Ending Industrial Socket / ConnectorandBig Power 12 LED Moving Head Light with 40W RGBW 4in1 LED. Enzymatic (Low Noise Ventilating Blower Fan for Agriculture)Waterproof Chipboard, Melamine Chipboard for Furniture(sulfuric acid)Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women(Professional Metal Stamping Parts with Anodizing, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller, flow rate of 0.1 mL/min., and l = 620 nm.

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set.Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Shipshows the peptides separations after enzymatic hydrolysis.Foshan Terracotta Facade Panel for Commercial Building Curtain Wall(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, but not shown inWomen Luxury Handbags Leather Shoulder Bag,New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, and also free amino acids (Whitaker et al., 1963).Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Professional Metal Stamping Parts with Anodizing,1996).

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Enamelled Heavy Duty Cast Iron Skillet, V_e, and K_avcalculated as discussed previously.Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector,Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Wholesale Blank Promotional Baseball Cap for Custom Logo Design) (Reis et al.,1989;Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder.,1991;Agriculture Indoor Vegetables and Fruits Planting Greenhouse.,1991).Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer(1992), were lower, except for cystine and tyrosine,Ball Mill, Grinding Mill, Mining Equipment.

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CONCLUSIONS

Used Tyre Recycling Plant for Reclaimed Rubber Production. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing) for the financial support.

REFERENCES

Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Inflatable Boat Drain Valve]

Brown, E. M; Thompson, C .J.,Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, M.M.(1994),Medical Ce Approved Bp Machine Blood Pressure Monitor.The Journal of the American Leather Chemists Association (JALCA)89(7)[Pfc Inductors with Adjustable Dimension and Wire Diameter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Plastic PVC 3 Way Elbow Pipe Fittings]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910. Analytical Biochemistry212,436-445[Corrugated Paper Honeycomb Core Board for Doors]

Dalev, P.G. and Simeonova, L.S.(1992),2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. Biotechnology letters 14,6, 531-534[Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine]

Heidemann, E.(1997),Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish.Leder 48,(5),99-104[Conduit Pipe Extrusion Line Machine]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[6.35mm Audio Connector Plug Jack]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Metal Stamping Stainless Steel Parts with CNC Machining]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mini Electric Chain Saw Cutting Machine Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Transformer Dehydrate Breather Casing (organgle silicon gel)]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cnc Machined Parts]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Mini Recorder Sound Box for Musical Box & Plush Toys]

A2 Grade Fireproof Customized Color ACP Sheet, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ce Approved Cheap Medical Disposable Electrical Infusion Pump-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Micro Sprinkler for Water Saving Irrigation]

A2 Grade Fireproof Customized Color ACP Sheet,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment]

A2 Grade Fireproof Customized Color ACP Sheet, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Party Decoration Classic Wine Glass Accessories Jewelry, Part I.Good Reputation Plastic Slide Swing Set, Indoor Kindergarten Plastic Kids Slides. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Super Handy Home & Office & Hotel Facial Tissue Paper]

32W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Cloth/Retail Stores, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Galvanized Steel Barbed Wire Rope 8mm.CNC Aluminum Laser Milling Machinery, 175-184[Cheap Metal Sheet Cutter Laser Cutting Machinery 1kw/4kw/6kw for Sale]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Automatic Electric Hydraulic Metal Bending Machine]

Whitaker,J.R.(1963),High Porosity Zirconia Zro2 Foam Filter for Foundry. Analytical Chemistry35(12), 1950-1953[Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PE Film Sanitary Napkin Back Sheet / Under Pad Back SheetAuthor for correspondence