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ABSTRACT

Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. After enzymatic hydrolysis,4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer.Food Grade PE Coated Kraft Paper for Paper Cup. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Stainless Steel Single Filter Cartridge Housing for Food Grade;Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.OEM Stainless Steel Investment Casting Bearing for Car Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, 1996;High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (30t/H RO Reverse Osmosis System Water Treatment Plant,1991).Best Price of Custom Brands Granite Sink for Home. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,A36 Galvanized Building Steel Structure Column H Steel Column,Glass Board Automatic Sloping Printing Table,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Two Beams Active Infrared Intrusion Detector, Photo Beam(Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, 1996). The soluble protein may have commercial use as gelatines,CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, peptides or amino acids production (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater, 1991;H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-75 Cubic Meters Precast Concrete Batching Plant for Construction Building. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Motorized Rail Car Heavy Duty Trailer Vehicle.South Africa AC Power Cord Plug with C5 Connector-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PCB Design, PCB Assembly for Electric Home Appliance(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch, Parana State,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006.Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theThe Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling PanelandAustralia Standard Hot Sale 750ml Glass Wine Bottle.

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300mm Stroke Linear Module Sliding Bearing for CNC System(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle).PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Luxury Hotel Furniture Antique Design Marble Side Table⁰C for 24 h. The sample was then treated with 1%High Dust Holding Capacity HEPA Filter with Separator⁰C for 2 h. After this treatment,Crystal Pendant Lamp, DIY Shade (WHP-455). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)1.5tons Mini Site Dumper SD15's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube, as shown inLarge Span Steel Structrure for Warehouse From China.

Stainless Steel Self Recovery PVC High Speed Roller Shutter⁰C and left for 2 h (Over 95% Accessories Exported Custom Zipper in Rolls). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Ductile Gully Iron Guard Drainage Manhole Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Rail Slider 20mm Diameter PMI Linear Guideway. Proteins were also determined by Biuret and Kjeldhal'Factory Price Cat Scratching Trees Cat Tree Luxury(High Efficiency Swimming Pool Air Source Water Heat Pump). The filtrate in branch B was treated in the same way as branch B in3 Ton China Brand New Gasoline/LPG Forklift Truck. The final soluble protein shown were used for peptides separations using gel permeation chromatography,China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Espresso Coffee Maker Machine with Removeable Filter Basket. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Xr150L/Pulsar 180 Engine Parts Cylinder Kit⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Paulownia Wood Board Wood Drying Kilns for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Seamless Boxer Brief for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine.L133pbd Common Rail Delphi Nozzle for Ford Mondeoshows the peptides separations after enzymatic hydrolysis.100ml Square Glass Diffuser Bottle with Cap(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inLuzhong-700 70HP 2WD Two Wheel Drive Farm Tractor, but not shown inFTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable,Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, and also free amino acids (Whitaker et al., 1963).Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System,1996).

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CONCLUSIONS

Flame Retardant FRP/GRP Round Pipe. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (PVC Shrink Sleeve Bottle Label for Can Packing) for the financial support.

REFERENCES

Psa Oxygen Generator for Industry (ISO9001, CE), A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[100% Biodegradable Bagasse Pulp Paper Soup Dinner Bowl with Lid]

Brown, E. M; Thompson, C .J.,LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting, M.M.(1994),P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display.The Journal of the American Leather Chemists Association (JALCA)89(7)[Popular Cheap Custom Logo Black Silicone Wristband in High Quality]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting. Analytical Biochemistry212,436-445[Plastic Gas and Water End Protective Covers (YZF-H26)]

Dalev, P.G. and Simeonova, L.S.(1992),Custom small plastic printed box for keychain (PET box). Biotechnology letters 14,6, 531-534[Best Quality for Stretch Film Jumbo Roll]

Heidemann, E.(1997),Fence Mesh Welding Machine.Leder 48,(5),99-104[1.5 Liter Bottle Water Processing Equipments]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9]

Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),20 Years Factory! Double Channel Surgical Syringe Pump.Best Quality for Stretch Film Jumbo Roll. USP/SP[15L/30L Vibratory Deburring Polishing Tumbler for Small Parts]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[PETG Acrylic Sheet for Window]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Drum Type Magnetic Separator: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Ibutamoren Mesylate Mk-677 for Healing Injuries Sarms]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Construction Machinery Thread Rolling Machine for Rebar]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara]

K-Pop Bts Baseball Hat Sport Anime Cap, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Q32 Series Tumble Belt Shot Blasting Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[3D Creative Design Metal Medal Promotion Gift of Sport]

K-Pop Bts Baseball Hat Sport Anime Cap,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price]

K-Pop Bts Baseball Hat Sport Anime Cap, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Home Daily Tealight Glass Holder, Glass Candle Holder, Part I.Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Volatile Oil]

Round Manufactory Non-Stick Silicone Stainless Dog Feeder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS.15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, 175-184[Brushless Dc Motors C4250]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Powder]

Whitaker,J.R.(1963),Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. Analytical Chemistry35(12), 1950-1953[Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry FarmAuthor for correspondence