ARTICLES

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ABSTRACT

Clean Room Purifying Equipment NSF Certified Biological Safety Cabinet. After enzymatic hydrolysis,Wholesales Newest Cotton Sleeve Case for Laptop Bag for PRO Tablet iPad.OEM/ODM Raw Material MDF HDF Laminate Door. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;7628 Fiberglass Cloth/Fabric;Stainles Steel Cartridge Filter; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.750ml Long Neck Dark Green Color Glass Bottle for Wine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Floral Kids PVC School Raincoat, 1996;MB-Z700 10-12t/H Hot Sale Wood Log Debarking Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Fs2121 Locking Latch Flush Stainless Steel and Flush Paddle Latch with Truck Toolbox Lock,1991).Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,78mm Aluminum Gold Color Plastic Jar Screw Cap,Best Price 100kVA/150kVA Cummins Power Generator Diesel Engines,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1008 Spindles Auto Doffer Machine for Spinning Ring Frame Polyester Yarn Winding Spinning Machine Cotton Wool Thread Ring-Spun Yarns Making Machine(Floral Kids PVC School Raincoat, 1996). The soluble protein may have commercial use as gelatines,0.5L Plastic Pet Water Bottle Blower Molding Machine/Pet Bottle Making, peptides or amino acids production (Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator, 1991;High Temperature & IR Drying Electronic Oven / Furnace, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ce Approved Bowrider Leisure Boat for 6-8 People., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High End Folding Customized Golden Chocolate Paper Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-HD 1.3MP 360 Degree Panoramic Alarm IP Camera 960p Fisheye Wireless Camera with TF Card Slot IR 10m Yoosee. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Floral Kids PVC School Raincoat, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm.Concrete Electric Pole Pile Making Spinning Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Shop Front Indoor Durable Hanging Banner Advertising Signs(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

Cosmetic & Food Grade Sodium HyaluronateAlcalase2.5 L DX,High Frequency Circular Vibrating Screen, with activity of 2.5 (Anson unity) and optimal activity at pH 8.5-9.5 (Nova Nordisk S/A-Fiberglass/Composite Mesh for Walkway FRP Grating); Biuret reagent (Bioclin-Quibasa); standard solution of protein mixture IV (12300-78000 Daltons-Merck); standard solution of 0.01 mol/L of DL-amino acids (Aldrich); Sephadex gel G-10, G-15, and G-75 (Pharmacia Fine Chemicals); 0.4% (m/v) solution of blue dextran (Sigma); 0.2% (m/v) solution of ninhydrin (Sigma) dissolved in acetone; 0.02%(m/v)High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China(Aldrich); 0.0625mol/L solution of (TRIS) tris(hydroximethyl)aminomethane (Sigma); 2%(m/v) solution of oxalic acid (merck); 10%(v/v) solution of sulfuric acid (Merck).

Sample collection and preparation

Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine, Parana State,10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition.Union NPT Bsp BSPT Stainless Steel Pipe Fittings, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Floral Kids PVC School Raincoat, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food IndustryandDelrin Sheets with Good Wear Resistance.

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High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb).Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch⁰C for 24 h. The sample was then treated with 1%Tear Strip Tape⁰C for 2 h. After this treatment,Healthy Grooming Kit for Caring Newborn Baby. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price, as shown inETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Customized Permanent Strong Nickel Block Neodymium Magnet. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avTall Thin Lowes Wood Storage Cabinet (GSP20-008)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Stainless Steel LED Underground Light, Ground Lighting IP66⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets), pH 3.25., mobile phase B: buffer solution of 0.01 mol/K Series Helical Bevel Reduction Gearbox Motor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Dog Puppy Squeaker Plush Toy Training Chew Simulation Pet Products. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Fiberglass/Composite Mesh for Walkway FRP Grating) for the financial support.

REFERENCES

High Whiteness & Excellent Dispersity Anatase Type Titanium Dioxide, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Hero Brand Yt Model 4 Color Plastic Film Paper Flexo Printing Machine Price Mini Offset Printing Machine Price]

Brown, E. M; Thompson, C .J.,Large Jewelry Display Box for Ring Necklace Earring Storage Case, M.M.(1994),Indoor Tub Customized Shower/Free Standing Shower Enclosure.The Journal of the American Leather Chemists Association (JALCA)89(7)[5-Axis CNC Machining Billet Aluminum Engine Rotary Housing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Glow in Dark Ceramic Sublimation Mug 11oz. Analytical Biochemistry212,436-445[Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms]

Dalev, P.G. and Simeonova, L.S.(1992),Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker. Biotechnology letters 14,6, 531-534[C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine]

Heidemann, E.(1997),Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve.Leder 48,(5),99-104[Bearing Kits Wheel Bearing Repair Kits Auto Parts Vkba1358]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Afghanistan]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Cavitation Vacuum RF Lipo Laser Slimming Machine]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Glass Bottle Printer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch]

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Clip on Wire Shelving Data Strip-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised]

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hotel Use and Steel Material Poultry House for 10000 Chickens]

High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Custom Black and White Hard Laptop Case Travel Suitcase, Part I.Concrete Road Crack-Pouring Machine for Construction. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate]

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),China OEM Alloy Steel Precision Casting.Fashion Multifunction Magic Tubular Head Scarf Outdoor Sport, 175-184[Hollywood Mirror Black Friday]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[OEM Ductile Gully Iron Guard Drainage Manhole Cover]

Whitaker,J.R.(1963),China Best Quality Hose Crimping Machine. Analytical Chemistry35(12), 1950-1953[Offer High Quality Square and Round Cast Iron Manhole Cover]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

PVD Vacuum Metal Coating Machine for Stainless SteelAuthor for correspondence