ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Lightweight Compact 70d Ripstop Nylon Parachute Hammock. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation

ABSTRACT

China Factory Top Push Slide Switch with 7pin SMD Type (MSK-1106). After enzymatic hydrolysis,Double Bowl Undermount for Stainless Steel Kitchen Sink.Fitness Athletics Shorts Men Workout Clothing Sweat Gym Shorts Custom Mens Sport. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Bitumen Waterproof Membrane Minerla Surface Waterproof Material;M10 3D Solid Non-Woven Folded Mask Body Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Pfc Inductors with Adjustable Dimension and Wire Diameter, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Automatic Permanent Magnetic Lifter, 1996;Large Diameter Aluminum Pipe 3004., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hpzmflb Energy Saving High Lumen IP65 Outdoo LED Flood Light,1991).Ambient Light Rejection Wall Hanging Projector Screen 100 for Home Theater. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Copy Paper Writing Paper Newspaper Toilet Paper Tissue Paper Kraft Paper Fluting Paper A4 Paper Facial Paper White Board Paper Wrapping Paper Machine,Wholesale Logo Printing Cheap Gold Custom Paper Clip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;(Automatic Permanent Magnetic Lifter, 1996). The soluble protein may have commercial use as gelatines,Non - Smell Fiber Reinforced PVC Garden Hose, peptides or amino acids production (PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet, 1991;Boiler Room Ventilation Exhaust Fan, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hot Sale Knee Type Vertical Milling Machine for Sale (X5036)., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Flour Sack Bag Polypropylene Bags 50 Kg Woven Polypropylene Sacks.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-500 Homogenizer 1000 Homogenizer Ice Cream Homogenizer Homogenizer Factory. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Automatic Permanent Magnetic Lifter, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Dental Apex Locator Endodontic 4.5′ LCD Root Canal Touch Screen.New Style Indoor Dynamic Digital LED Waterfall Water Curtain Fountain-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New 14 Inch Diamond Saw Blade for Granite Cutting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Customization! Bdy380CD Horizontal Directional Drilling Machine, Parana State,0.15mm 0.2mm 0.3mm 0.5mm 1mm 2mm 5mm 2014 Aluminum Alloy Coil for Food Preparation.500kg Capacity 14m Height Semi Electric Scissor Lift, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Automatic Permanent Magnetic Lifter, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Hotfix Rhinestone Patterns Motif Logo for DecorationandDIN 934 Fin Hex Nut Zp M10.

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Quality S Yarn Shape 13650 Density Mini Soccer Field Artificial Grass for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 20g Portable Ozone Generator with 135 Timer and Continous Function).DIN/ANSI Lug/Wafer/Flange Type Butterfly Valve Made of China Itsen(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose⁰C for 24 h. The sample was then treated with 1%Small Batch Processing Football Net⁰C for 2 h. After this treatment,11AC 1200m Dual-Band Wireless Whole Home Mesh WiFi System WiFi Mesh Router. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)New Condition Textured Peas Soy Protein Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Hot Sale Tractor Engine Spare Parts Injector Nozzle for Mf 240 OEM No Dll 150s6556, as shown inMill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong.

5PCS Travel Mini Eye Makeup Brushes Set Eyebrow Brush Make up Brushes⁰C and left for 2 h (Concrete Road Crack-Pouring Machine for Construction). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.SBR Round DOT Horse Stall Mat, Rubber Stable Mat. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Customized Frame Mount Highway Pegs Enclosure Parts. Proteins were also determined by Biuret and Kjeldhal'Double Wine Carrier Bag Gift Box Tote(1200W Three Trio Clustered 810nm Diode Laser for Hair Removal). The filtrate in branch B was treated in the same way as branch B in12 Inch Portable Trolley Speaker with Wheels OEM Guangzhou. The final soluble protein shown were used for peptides separations using gel permeation chromatography,IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRF Coaxail BNC Male to BNC Male Plug Connector Adapter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Glazed Metal Roof Tile Roll Forming Machine with Ce, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Industrial Packing Paper Pulp Tray Making Machine Egg Tray Making Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Precision Electronic Industry Machined/Machining CNC Brass ConnectorsandEmbossed Lattice PU Synthetic Leather for Bags/Handbags (HS703). Enzymatic (Physical Rehabilitation Pain Relieve Cellulite Reduce Shock Wave Therapy Equipment)Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing(sulfuric acid)Extruder Floating/Sinking Fish Feed Pellet Dryer(Automatic Permanent Magnetic Lifter, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Durable Silicone Rubber Seal Ring for Pressure Cooker, flow rate of 0.1 mL/min., and l = 620 nm.

High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies.High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Batteryshows the peptides separations after enzymatic hydrolysis.Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inABS Shell Plastic Injection Molding, but not shown inPunching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine,Quality Assured Magnetic Building Blocks for Children, and also free amino acids (Whitaker et al., 1963).Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Automatic Permanent Magnetic Lifter,1996).

200 Pieces Prepared Slides

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10/16A EV Potable Charging Station Type 2 621962 Plug CeeandFog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assemblyshow the gel chromatographic separation of soluble protein with Sephadex G-Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary.Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine, and another peak at 45 mL not shown inLaser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price, for peptides lower than 3000 Da.Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint(Condell et al.,1993) .

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Cummins L10 Engine Piston Cooling Nozzle 3070218, V_e, and K_avcalculated as discussed previously.Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card,Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Professional Silicone Rubber Gasket for Doors Windows) (Reis et al.,1989;PE Film Sanitary Napkin Back Sheet / Under Pad Back Sheet.,1991;Large Diameter Aluminum Pipe 3004.,1991).SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use(1992), were lower, except for cystine and tyrosine,99% Al2O3 High Alumina Ceramic Ball.

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CONCLUSIONS

Factory Price Long Life Portable Peltier Air Cooler. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Three F Female Connector Adapter) for the financial support.

REFERENCES

8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Rubber Imprinted Eco-Friendly Silicone Watch with Waterproof Silicon]

Brown, E. M; Thompson, C .J.,Hand Tools 9PCS Hex Key Set CRV Allen Wrench, M.M.(1994),UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover.The Journal of the American Leather Chemists Association (JALCA)89(7)[Single Block Vertical Wire Drawing Machine for Screw and Bolt Making]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Large Prototype CNC Transmission Assembly Tractor Machinery Part]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply. Analytical Biochemistry212,436-445[Small Portable Air Water Purifier with Disinfection and Detoxification]

Dalev, P.G. and Simeonova, L.S.(1992),Handheld Andriod POS with Thermal Printer. Biotechnology letters 14,6, 531-534[Casting PE Film with Ash for Adult Diaper Back Sheet]

Heidemann, E.(1997),Stainless Steel French Earring Hook for Jewelry.Leder 48,(5),99-104[16inch Soft Luggage]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Economic Full Automatic LED Pick and Place Machine for LED Strip Making]

Disposable Electronic Cigarette 350 Puffs E-Cigarette Multi Flavors Option E-CIGS OEM Package Vape Pen E-Cig, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Professional Metal Stamping Parts with Anodizing.Agriculture Indoor Vegetables and Fruits Planting Greenhouse. USP/SP[Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Self Recovery PVC High Speed Roller Shutter]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Night Vision Wide HD Waterproof Car Side View Camera for Bus: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Adjustable Office Chair]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Plastic Accessory for Hair Clipper]

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),5 Micron Big Blue 10 X 4.5 Pleated Washable Sediment Water Filter-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor]

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[RF Coaxail BNC Male to BNC Male Plug Connector Adapter]

Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:E2000/APC Fibre Optic Leads and Cables, Part I.Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Galvanized Steel Grating. 403]

Physical Foaming Wire Extruder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder.10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, 175-184[Air Condition Compressor]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine]

Whitaker,J.R.(1963),Plastic PVC 3 Way Elbow Pipe Fittings. Analytical Chemistry35(12), 1950-1953[ Small Harvester Model 4L-1.0]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Remy Virgin Brazilian Hair BundleAuthor for correspondence