ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,450mm High Quality Circular Diamond Cutting Saw Blade for Marble Stone

ABSTRACT

Manual Glass Lifting Machine for Making Double Glass. After enzymatic hydrolysis,Aluminium Profile Heavy Duty Bending Machine Aluminum Window Door Machine.Towed Bitumen Mixer Slj5. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High Adsorption Capacity and Porosity Chloride Adsorbent Activated Alumina Price;3D Inkjet Printing Waterproof Interior Kitchen Wall Tile; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, 1996;Super Absorbent Lint Free Clean PCB Double Foam Tip Swab Stick., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Viscose Sodium Sulphate Anhydrous by Sateri,1991).Orange Silica Gel 2-4 mm with High Quality. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Top Quality USB-C Docking Station with SD Card Reader Dp VAG HDMI,aluminium discs for fry pan alloy 1060 1100 1050 3003,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Insulated Double Wall Bamboo Glass Water Bottle(T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, 1996). The soluble protein may have commercial use as gelatines,Aroma Reed Diffuser with Ratten Sticks for Home Fragrance, peptides or amino acids production (High Quality Fashion Ladies Backpack PU Leather Backpack, 1991;Suction Filter Cylinder Match 48 Series Filter Brands, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;I10 Tws Mini Bluetooth 5.0 Earphone Twins Wireless Headphones Air Pods in Ear Earbuds Sport Headset Stereo for Apple iPhone Samsung Xiaomi., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.16oz Square Shape Colors Glass Mason Jar with Embossed Design.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Aerobics and Exercise Highly Durable Floors of Offices and Commercial Rooms.100ml Plastic E Liquid Vape Cig Juice Dropper Bottle-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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12V/24V, Ts16949, Air Conditioner Fan for Difference Refrigeration Truck(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger).nellcor non-oximax 7pin infant disposable sensor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Power Solar Inverter with Full Power 3000W Converter⁰C for 24 h. The sample was then treated with 1%Custom Made EPDM FKM Rubber Mechanical Seal Diaphragm for Valve⁰C for 2 h. After this treatment,Customized OEM Oil Filter/Air Filter Aluminum Parts Die Casting for Engine Parts/ Car Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Zoo-Tunes Portable Wireless Mini Character Speakers's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CF8m Air Actuated Valve Dn15, as shown inNPK compound fertilizer granulating machine.

Rectangular Design Ceramic Small Glass Basin Sink for Bathroom⁰C and left for 2 h (Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Premium Baby Toddler Food Feeding Silicone Bowl with Lid. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Hardcover Bear′s Story Children Book Custom Printing. Proteins were also determined by Biuret and Kjeldhal'Deluxe Saddle Doctor Chair Dental Stool with Adjustable Seat(7628 Fiberglass Cloth/Fabric). The filtrate in branch B was treated in the same way as branch B inSpark Tester For Wire And Cable. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Medium High Carbon Black Round Bicycle Wheel Spoke Tension Steel Wire.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Liquid Hardener Setting Cement Membrane Curing Concrete. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avProfessional Silicone Rubber Gasket for Doors Windows(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Flat Face Stainless Steel Female Jic Quick Release Coupling, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Aluminium Glass Dropper for Glass Dropper Bottle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ge Holter Cable Snap Type Holter ECG Cable.NBR EPDM Neoprene Silicone Rubber Sheet for Sealshows the peptides separations after enzymatic hydrolysis.Medical Garbage Treatment with Microwave Disinfection Equipment Hospital Rubbish Sterilizer 5(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHaivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), but not shown inLarge Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid,PVD Vacuum Metal Coating Machine for Stainless Steel, Ceramic, Glass, and also free amino acids (Whitaker et al., 1963).65 Polyester 35 Cotton Fabric Camouflage Printed Fabric. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (T5577 Thick Card T5577 Security Smart Chip RFID ABS Plastic Blank Thick Card,1996).

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CONCLUSIONS

Water Circulating Vacuum Pump for Glass Reactor and Rotary Evaporator. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Engraving Machine Laser Marking for Assembly Line) for the financial support.

REFERENCES

Ce Jacket Heated Water Chocolate Holding Tank Manufacturer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Magnetic Separators-Magnetic Liquid Traps]

Brown, E. M; Thompson, C .J.,Lanyards Ribbons Screen Printing Machine, M.M.(1994),China Factory New-Product-Best-Quality-Customized-Metal-Pressing.The Journal of the American Leather Chemists Association (JALCA)89(7)[PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Outdoor P5mm Programmable Bus LED Display Sign]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Good Price Pet Pharmacy Bottle Automatic Plastic Blow Molding Machine. Analytical Biochemistry212,436-445[Eco-Friendly Round Head Screw Stud Flange Hexagon Bolt for Building]

Dalev, P.G. and Simeonova, L.S.(1992),Recycle Finger Joint Core Film Faced Shuttering Plywood. Biotechnology letters 14,6, 531-534[High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087)]

Heidemann, E.(1997),Excavator Diesel Engine Spare Parts Yanmar 4tnv98 Main Bearing+0.5.Leder 48,(5),99-104[High Quality Ce Approved Q35y-30 Iron Worker Shearing and Punching Hydraulic Ironworker]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Food Grade PE Coated Kraft Paper for Paper Cup]

ABS Shell Plastic Injection Molding, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Beer Made in China OEM Abv 8.0%Vol Lager Beer.Cat Cardboard House Pet Product Accessories. USP/SP[Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-EPDM Crossfit Gym Mat/Rubber Interlocking Flooring: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Electrical Waterproof Pg11 Cable Gland Plastic Types with IP68]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen]

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hf Vacuum Dryer Machine for Drying Wood-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine]

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Oval Shape Cast Iron Dutch Oven]

Aoer 1/3HP, 1725 Rpm, 115V AC Single Phase Electric Motor Washing Machine Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table, Part I.52cc Gasoline Paddy Cutter Two Handle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm)]

Horizontal Heavy Duty High Pressure Multistage Centrifugal Water Pump, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Durable Fully Automatic Hot Dipped Anti-Rust Galvanized Vertical Type Wire Mesh Steel Metal Baby Chicks Breeding Cage.OEM Viton Mechanical Rubber Oil Seal, 175-184[M3/M6/M10/M15/M20/Mx20/Mx25/Ts6/Ts20/Tl10/T20 Yojo K60b Gasket Plate Heat Exchanger HVAC Marine Heat Exchanger Gasket Plate]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale Starter Motor Testing Equipment for Truck]

Whitaker,J.R.(1963),Rapid Prototype Plastic Molding Simple Rapid Tooling and Simple Mould. Analytical Chemistry35(12), 1950-1953[High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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