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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, 1996;Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844),1991).Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Single Block Vertical Wire Drawing Machine for Screw and Bolt Making,High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,China Electric PCB Circuit Board with High Quality(Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, 1996). The soluble protein may have commercial use as gelatines,OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts, peptides or amino acids production (Anodized Perforated 3D Facade Windows Screen for Commercial Building, 1991;Full Cream Milk Powder Filling Machine (XFF-G), 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat.Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PVC Car Seat Belt Shoulder Protector 80438(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set).Gold Open Metal Short Crocodile Wallet for Girl Women Lady(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor⁰C for 24 h. The sample was then treated with 1%Pond Self Priming Booster suction  Water Pump with Pressure Sensor⁰C for 2 h. After this treatment,FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)95g 3D Tooth Stain Remover Whitelock Toothpaste's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats, as shown inCustom Size Rare Earth Permanent Speaker Ring Ferrite Magnet.

Al/Pet/Al Double Sides for Aluminium Flexible Air Duct⁰C and left for 2 h (Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.European Standards 100A 24V Electrical Relay for Green Energy. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal. Proteins were also determined by Biuret and Kjeldhal'Canvas Shoulder Weekend Shopping Big Bag(42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor). The filtrate in branch B was treated in the same way as branch B inSrdt Range Cord UL Approval UL ETL 12/3 12/4 10/3. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Visuable Reflective Tape for Vehicle Road Safety.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Made in China Aluminum Extrusion Window and Door Profile. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIndustrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.304/304L 2b Stainless Steel Sheet and Steel Plate⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Plastic Injection for OEM Product, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine.Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facilityshows the peptides separations after enzymatic hydrolysis.Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPVC Car Seat Belt Shoulder Protector 80438, but not shown in Anti-Slip Hot Sale Floor Mat,Portable Ink Date Printing Machine for Plastic Bag, and also free amino acids (Whitaker et al., 1963).High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops,1996).

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CONCLUSIONS

Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality 20PCS Colorful Makeup Brush Set) for the financial support.

REFERENCES

Hot-Sale Products GRP Tower for New Energy Industry, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[m.pointsourcingservices.com]

Brown, E. M; Thompson, C .J.,Fgg 2b 304 Metal Circular Push Pull Connector, M.M.(1994),Thick Wall Large Diameter Aluminium Tube Manufacture.The Journal of the American Leather Chemists Association (JALCA)89(7)[Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Pillow Case Automatic Sewing Machine Bc901]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Portable Hydraulic Power Pack 12V Hydraulic Power Unit. Analytical Biochemistry212,436-445[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

Dalev, P.G. and Simeonova, L.S.(1992),China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW. Biotechnology letters 14,6, 531-534[High Pressure Low Volume 2900rpm electric Water Pump]

Heidemann, E.(1997),52cc Petrol Brush Cutter for DIY Garden Care.Leder 48,(5),99-104[Airshield Temperature Probe Suppliers for Sale]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fire Retardant Reflective Aluminium Foil Woven for Insulation Material]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[CNC Machinery Aluminum and ABS Mock up Prototypes]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hans Custom Manufactured Smoothness Zipper Waterproof: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Thick Wall Large Diameter Aluminium Tube Manufacture]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[www.flsygroup.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)]

Quartz Liquid Powder Feeding Torch, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing]

Quartz Liquid Powder Feeding Torch,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Commercial Vegetable Drying Equipment]

Quartz Liquid Powder Feeding Torch, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, Part I.Outdoor P5mm Programmable Bus LED Display Sign. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Capacitive Push Button Membrane Keyboards]

AGM Separator for AGM Battery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Electric Battery Flameless LED Tea Light.42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, 175-184[www.bobhouseware.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Whitaker,J.R.(1963),CE Certificated Wheel Loader (HQ912) with Agricultural Tire. Analytical Chemistry35(12), 1950-1953[Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Galvanized Metal Welded PVC Coated Wire Mesh FenceAuthor for correspondence