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ABSTRACT

Drum Type Magnetic Separator. After enzymatic hydrolysis,K-Pop Bts Baseball Hat Sport Anime Cap.Q32 Series Tumble Belt Shot Blasting Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Home Daily Tealight Glass Holder, Glass Candle Holder;Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Round Manufactory Non-Stick Silicone Stainless Dog Feeder, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, 1996;15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors,1991).Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid,High Lumen Outdoor IP65 100W LED Flood Light,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality(Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, 1996). The soluble protein may have commercial use as gelatines,Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, peptides or amino acids production (European Fashion Style Ladies Wrist Watch with Interchangeable Strap, 1991;Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Full Automatic Combination Weighing Packing Machine Jy-420A.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tfl Blue Rubber Transmission Belts/Automotive Spare Part.1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Flat Wedge Wire Panel China Factory(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

New Design Disposable Trocars for Laparoscopic Surgery, Parana State,Best Quality for Stretch Film Jumbo Roll.Disposable Medical Combined Bacterial Viral Hme Filter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theSc/APC Fiber Optic Fast Hot Melt ConnectorandBsp 60° Male Cone Seat Carbon Steel Fittings.

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Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Industrial Low Temperature Water Cooled Scroll Water Chiller).Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb⁰C for 24 h. The sample was then treated with 1%High Efficiency Pineapple Canning Plant⁰C for 2 h. After this treatment,Embossed Pattern PU Leather Classic Mens Wallet. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Casting PE Film with Ash for Adult Diaper Back Sheet, as shown inRibbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids.

Supra Brand Laundry Liquid Baby Formula⁰C and left for 2 h (Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cleaning Products Sponge Vacuumized Pack 65mm. Proteins were also determined by Biuret and Kjeldhal'Mineral Water Bottle Mould Plastic Blowing Mold(Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film). The filtrate in branch B was treated in the same way as branch B in416 Grade Ss Round Bar. The final soluble protein shown were used for peptides separations using gel permeation chromatography,High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avClass 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Building Material Quick Install Types of Scaffolding Couplers⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Folded Kitchen Towel Paper Wrapping Packaging Machine.High Quality Bucket Segment Bolt 5p7665shows the peptides separations after enzymatic hydrolysis.Cheap Topmac Brand Mobile Concrete Mixing Plant(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBuilding Material Quick Install Types of Scaffolding Couplers, but not shown inTs-Micro Portable Defense Loudspeaker System,Color Stretch Film for Pallet Wrap Use, and also free amino acids (Whitaker et al., 1963).Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS,1996).

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3D Creative Design Metal Medal Promotion Gift of Sport, V_e, and K_avcalculated as discussed previously.HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line,Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Custom Black and White Hard Laptop Case Travel Suitcase) (Reis et al.,1989;European Fashion Style Ladies Wrist Watch with Interchangeable Strap.,1991;15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.,1991).Composite Plate Dedicated Drop Hose Gear Metering Pump Price(1992), were lower, except for cystine and tyrosine,Brake Fluid Tank Container Hot Plate Plastic Welding Machine.

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CONCLUSIONS

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector) for the financial support.

REFERENCES

Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Portable Type Home Use 55kVA Cummins Silent Diesel Generator 44kw Generator]

Brown, E. M; Thompson, C .J.,Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, M.M.(1994),High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts.The Journal of the American Leather Chemists Association (JALCA)89(7)[Superior Rubber Lining Curing Press/Rubber Lining Plate Vulcanizing Press]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Velvet Jewelry Box Gift Box with Bowknots for Packing]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Puffed Core Filling Sanck Production Machine with Ce From China. Analytical Biochemistry212,436-445[Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument]

Dalev, P.G. and Simeonova, L.S.(1992),86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting. Biotechnology letters 14,6, 531-534[Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush]

Heidemann, E.(1997),Cummins Fuel Injector Kit (3076736 3095023).Leder 48,(5),99-104[China Wood Milling]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 115V AC Single Phase Electric Motor Washing Machine Motor]

Fence Mesh Welding Machine, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5.Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch. USP/SP[Flame Retardant FRP/GRP Round Pipe]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Therm Forming Filling Sealing Machine, Lid Press Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Vacuum packer]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[50d Cationic Spandex Stretch Fabric for Garment]

Electric 100W Three Wheels Toy Car for Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),New Design Customized 100% Cotton Home Towel Bath Towel-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ]

Electric 100W Three Wheels Toy Car for Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Construction & Decoration ERW Square Steel Tube Manufacturer]

Electric 100W Three Wheels Toy Car for Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Toughened Railing Glass, Part I.Delrin Sheets with Good Wear Resistance. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Fiber Reinforced Plastic GRP FRP Fiberglass Gratings]

High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service.Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies, 175-184[Oscillating Knife Cutting Machine EVA/Foam/Cardboard Cutter Plotter]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer]

Whitaker,J.R.(1963),Plastic Gas and Water End Protective Covers (YZF-H26). Analytical Chemistry35(12), 1950-1953[SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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