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ABSTRACT

Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. After enzymatic hydrolysis,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors.30t/H RO Reverse Osmosis System Water Treatment Plant. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Glass Board Automatic Sloping Printing Table, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Two Beams Active Infrared Intrusion Detector, Photo Beam, 1996;CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater,1991).H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry,Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,75 Cubic Meters Precast Concrete Batching Plant for Construction Building(Two Beams Active Infrared Intrusion Detector, Photo Beam, 1996). The soluble protein may have commercial use as gelatines,Motorized Rail Car Heavy Duty Trailer Vehicle, peptides or amino acids production (South Africa AC Power Cord Plug with C5 Connector, 1991;PCB Design, PCB Assembly for Electric Home Appliance, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.NPK compound fertilizer granulating machine, reduce dust pollution.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Shrink Sleeve Bottle Label for Can Packing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Two Beams Active Infrared Intrusion Detector, Photo Beam, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable.Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Crystal Pendant Lamp, DIY Shade (WHP-455), Parana State,OEM Stainless Steel Investment Casting Bearing for Car Parts.1.5tons Mini Site Dumper SD15, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Two Beams Active Infrared Intrusion Detector, Photo Beam, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHardcover Bear′s Story Children Book Custom PrintingandMineral Water Bottle Mould Plastic Blowing Mold.

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1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Best Items Gh/Genatropin with Naked Vial).Stainless Steel Self Recovery PVC High Speed Roller Shutter(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Ductile Gully Iron Guard Drainage Manhole Cover⁰C for 24 h. The sample was then treated with 1%High Precision Rail Slider 20mm Diameter PMI Linear Guideway⁰C for 2 h. After this treatment,Factory Price Cat Scratching Trees Cat Tree Luxury. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Espresso Coffee Maker Machine with Removeable Filter Basket, as shown in38X72X37mm Dac38720037 Wheel Hub  Bearing  for Motor Vehicle.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSilent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Lime Dewatering Filter Press Cloth Filter⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/150ml Round Aromatherapy Diffuser Glass Bottle, pH 3.25., mobile phase B: buffer solution of 0.01 mol/C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Fence Mesh Welding Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Luxury Hotel Furniture Antique Design Marble Side Table) for the financial support.

REFERENCES

20 Years Factory! Double Channel Surgical Syringe Pump, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[2019 Best Cordless Hair Trimmer Men Rechargeable Hair Clippers]

Brown, E. M; Thompson, C .J.,Best Quality for Stretch Film Jumbo Roll, M.M.(1994),Drum Type Magnetic Separator.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quality OEM Stainless Steel Casting Rings]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Indoor Tub Customized Shower/Free Standing Shower Enclosure]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-K-Pop Bts Baseball Hat Sport Anime Cap. Analytical Biochemistry212,436-445[Poultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer]

Dalev, P.G. and Simeonova, L.S.(1992),Q32 Series Tumble Belt Shot Blasting Machine. Biotechnology letters 14,6, 531-534[Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles]

Heidemann, E.(1997),Home Daily Tealight Glass Holder, Glass Candle Holder.Leder 48,(5),99-104[m.qingdaoflrope.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[China Manufacturer Multi Span Polycarbonate Industrial Green House]

Stainless Steel Single Filter Cartridge Housing for Food Grade, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant.Round Manufactory Non-Stick Silicone Stainless Dog Feeder. USP/SP[Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[20399560 Oil Seal 125X148X9.7 for Volvo]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Organic Fertilizer Black Shiny Granular 85%Min Water Soluble Potassium Humate From Leonardite]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Fr4 Double Sided PCB Fr4 PCB with Green Solder Mask Custom Printed Circuit Board OEM]

15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop]

15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Colorful Asphalt Shingle for Roof Water Proof]

15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10), Part I.Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Composite Plate Dedicated Drop Hose Gear Metering Pump Price]

High Lumen Outdoor IP65 100W LED Flood Light, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Yk-11 Yk11 Powder Yk 11 Sarm Powder in High Quality.Retail Supermarket Display Refrigerator Supermarket Beverage Showcase, 175-184[CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Whitaker,J.R.(1963),European Fashion Style Ladies Wrist Watch with Interchangeable Strap. Analytical Chemistry35(12), 1950-1953[Flat Wedge Wire Panel China Factory]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless MotorsAuthor for correspondence