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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Apex Digital UV Printers for Aluminum Board Printing UV6090. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,24 Molds Commercial Ice Cream Cone Maker

ABSTRACT

Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine. After enzymatic hydrolysis,Fashion Children Fur Pompom Beanie Scarf Knit Set.Steel Jacket Steam Insulation Pipeline Production Best Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Haa 800mAh NiMH Battery Pack;Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Electronic Muscle Stimulator EMS Machine for Slimming, 1996;Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover,1991).Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Custom Precision Aluminum Die Casting for Bicycle Accessories,China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain(Electronic Muscle Stimulator EMS Machine for Slimming, 1996). The soluble protein may have commercial use as gelatines,2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker, peptides or amino acids production (1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, 1991;Australia Standard Hot Sale 750ml Glass Wine Bottle, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.5000 Series Aluminium Disc/Circle 5005/5052/5083/5754.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Electronic Muscle Stimulator EMS Machine for Slimming, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Square Vibrating Sieve Machine, Linear Vibrating Screen.Manufacturers in China Ceramic Injection Moulding Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Over 95% Accessories Exported Custom Zipper in Rolls, Parana State,24 Molds Commercial Ice Cream Cone Maker.Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Electronic Muscle Stimulator EMS Machine for Slimming, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W HighbayandHigh Precision iPhone Cases Fiber Laser Marking Machine.

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China 100% PU Synthetic Leather for Shoes(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin).HD Video Wall P3.91 LED Panel Display Indoor(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%OEM Metal Stamping Stainless Steel Parts with CNC Machining⁰C for 24 h. The sample was then treated with 1%FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure⁰C for 2 h. After this treatment,Epoxy Resins. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)24 Cores MPO/MTP Cassette, as shown inCustomized CNC Machining Plastic Rapid Prototype for Medical Tool Box.

Automatic Concrete Paver Interlocking Brick Block Making Machine⁰C and left for 2 h (Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Lightweight Design Industrial Robot with Six Axis for Painting. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Small Diameter Coil Compression Spring From Factory. Proteins were also determined by Biuret and Kjeldhal'FDA Standard BBQ Skewer with Colorful Wooden handle(Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric). The filtrate in branch B was treated in the same way as branch B inGI/GL DX51D Z275 cold rolled hot dipped galvanized steel coil. The final soluble protein shown were used for peptides separations using gel permeation chromatography,2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Children Shoulder Backpack Bag Leisure School Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avAutomotive ECU Wire Harness 24 Pin Grey Connector(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Essence Oil Amber Glass Bottle with Plastic Dropper⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Metal Unfolding Grocery Push Carts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel SupplyandAuto/Manual Spray Painting Booth/Cabinet with Best Price. Enzymatic (DC 12V Garden Low Noise Submersible Water Return Amphibious Pumps Flow 3m)2019 Dotted Navy Rucksack Children School Bag Cotton Backpack(sulfuric acid)China Manufacturer Multi Span Polycarbonate Industrial Green House(Electronic Muscle Stimulator EMS Machine for Slimming, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Custom Plastic Injected (injection) Parts Molding Process, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Custom Plastic Injected (injection) Parts Molding Process, flow rate of 0.1 mL/min., and l = 620 nm.

4mm 6mm2 Soler Extension Leads with Mc4.Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furnitureshows the peptides separations after enzymatic hydrolysis.E0303 300n Activator Chiropractic Impulse Adjusting Instrument(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSpiral Rib Dies, but not shown inHuman Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression,Men′s Black and Grey Stripe Bamboo Dress Sock, and also free amino acids (Whitaker et al., 1963).CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Electronic Muscle Stimulator EMS Machine for Slimming,1996).

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Pet Dryer Machine /Pet Dryer Box/ Pet Hair Dryer, V_e, and K_avcalculated as discussed previously.Rubber Seal for Crank Box of Hks Warp Knitting Machine,OSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator) (Reis et al.,1989;1200W Three Trio Clustered 810nm Diode Laser for Hair Removal.,1991;Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail.,1991).Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable(1992), were lower, except for cystine and tyrosine,New Fashion TPU Mobile Cover Case for Huawei, Leather Phone Case for Huawei Mate 20.

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CONCLUSIONS

Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets) for the financial support.

REFERENCES

Extruded Polystyrene Foam Board Custom Edges S/L, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Custom Brass Rivet for Bag Copper Rivet for Clothing]

Brown, E. M; Thompson, C .J.,Hite 200tc Capsule Hotel Motel Bedding Plain Bed Sheet Sets, M.M.(1994),Magnetic Wireless Headset Metal Sports Bluetooth Headphone in-Ear Earphone Tws Wireless Earbuds Mini Bluetooth Stereo Headphone Mobile Earphone.The Journal of the American Leather Chemists Association (JALCA)89(7)[Manual Glass Lifting Machine for Making Double Glass]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8/16 Stands Polyethylene Hollow Braided Rope. Analytical Biochemistry212,436-445[High Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Hahal Duck Slaugter Processing Equipment for Poultry Farm Abattoir Machinery. Biotechnology letters 14,6, 531-534[Men Genuine Leather Sling Shoulder Satchel Messenger Bag]

Heidemann, E.(1997),NBR EPDM Neoprene Silicone Rubber Sheet for Seal.Leder 48,(5),99-104[Womens Sunglasses]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Community IC Access Card Induction ID Time Card Smart Card]

Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Commercial Coca Cola Refrigerator with Glass Door.Flyscreen Anti-Mosquito Mesh18*16/Inch Government Company. USP/SP[Automatic Waste Paper Stripper]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Double Jacketed Cooking Kettle Electric Jacket Boiler: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Extruder Floating/Sinking Fish Feed Pellet Dryer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Band Width 9.7mm-11.7mm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line]

Fashion Gray Leggings Stretch Fitness Work out Pants for Women, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hot Sale/HPMC/Clear/Empty/Vegan/Vegetable/Gel/Hard/Pullulan/Vegan Capsules-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine]

Fashion Gray Leggings Stretch Fitness Work out Pants for Women,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturers in China Ceramic Injection Moulding Machine]

Fashion Gray Leggings Stretch Fitness Work out Pants for Women, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:High Quality Wafer Type EPDM/NBR Seat Fluorine Lined Butterfly Valve, Part I.Medical Grade Ventilator for Sleep Apnea Breathing Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Qi Wireless Charger Portable Charger10W Fast Charging for iPhone/Sumsung /LG/Lenovo/Mi/Nokia]

UV Resistant Single Component Water Based Polyurethane Waterproof Coating, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Steel Rubber Seal for Dust Proof.Industrial Steel Structure Mezzanine Rack Platform System/Book Shelf, 175-184[Custom Gym Towel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Porosity Zirconia Zro2 Foam Filter for Foundry]

Whitaker,J.R.(1963),Kindergarten Playground Preschool Kids Plastic Slide and Swing. Analytical Chemistry35(12), 1950-1953[Gt4b63 Can Seamer Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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