ARTICLES

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ABSTRACT

High Precision iPhone Cases Fiber Laser Marking Machine. After enzymatic hydrolysis,Auto Water Cooling Radiator Release Temperature Screw Air Compressor.Injection Mold Maker for Plastic Fighter Toy. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;PCBA SMT Equipment PCB Depaneling Machine;HDPE Pipe for Water Supply (OD: 16mm-800mm); chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (2019 Male & Female Stainless Steel Quick Coupling, 1996;Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Digital Floating Baby Care Products Frog Bath Design Thermometer,1991).Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board,FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Colorful Asphalt Shingle for Roof Water Proof(2019 Male & Female Stainless Steel Quick Coupling, 1996). The soluble protein may have commercial use as gelatines,Durable Inline Hockey Skate Puck, peptides or amino acids production (Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane), 1991;18mm Thickness Best Quality Poplar Birch Commercial Plywood, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Nylon Hammer Fixings Drive Anchor. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (2019 Male & Female Stainless Steel Quick Coupling, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Electrolyte Effervescent Tablet.Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Manual Electric Winch Small Winch 220V Winch, Parana State,Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (2019 Male & Female Stainless Steel Quick Coupling, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3kw 4kw 5kw off Grid Solar InverterandManufacturer Automotive CNC Machined Parts Checking Fixture Components.

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Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Custom Velvet Jewelry Box Gift Box with Bowknots for Packing).Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood⁰C for 24 h. The sample was then treated with 1%Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part⁰C for 2 h. After this treatment,Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, as shown inMost Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument.

5-Axis CNC Machining Billet Aluminum Engine Rotary Housing⁰C and left for 2 h (Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box. Proteins were also determined by Biuret and Kjeldhal'Fashionable Simple Printing Mini Key & Coin Purse( 115V AC Single Phase Electric Motor Washing Machine Motor). The filtrate in branch B was treated in the same way as branch B inBrushless Dc Motor Driver. The final soluble protein shown were used for peptides separations using gel permeation chromatography,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Kids Folding Play Mat Kids Play Room Floor Mat. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avRoller Window Shutter for Residential Usage(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Hydraulic Hose SAE 100 R1AT, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Flame Retardant FRP/GRP Round PipeandShacman F3000 420HP 6X6 Tractor Truck with Cummins Engine. Enzymatic (L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07)Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(sulfuric acid)Ts-Micro Portable Defense Loudspeaker System(2019 Male & Female Stainless Steel Quick Coupling, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm, flow rate of 0.1 mL/min., and l = 620 nm.

Medical Supply for Laparoscopic Instrument (MIM Straight Scissors).40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rollsshows the peptides separations after enzymatic hydrolysis.86bl3a125 1.65A 660W 310V BLDC(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh, but not shown in50d Cationic Spandex Stretch Fabric for Garment,Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, and also free amino acids (Whitaker et al., 1963).530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (2019 Male & Female Stainless Steel Quick Coupling,1996).

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Best Price of Peanut Digger / Groundnut Harvester in Australian Africa, V_e, and K_avcalculated as discussed previously.China Manufacture Table Type CNC Horizontal Boring Milling Machine,White Electrical Fireplace Warmer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant) (Reis et al.,1989;Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane).,1991;Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys.,1991).Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor(1992), were lower, except for cystine and tyrosine,Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod.

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CONCLUSIONS

4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (304 321 310 310S Welded Decorative Stainless Steel Pipe) for the financial support.

REFERENCES

1 Inch Stainless Steel Flexible Exhaust Pipe, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[High Quality Mini Fridge Minibar]

Brown, E. M; Thompson, C .J.,Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403, M.M.(1994),Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio.The Journal of the American Leather Chemists Association (JALCA)89(7)[Cog Mono Round Display LCD Module]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator. Analytical Biochemistry212,436-445[Tfl Blue Rubber Transmission Belts/Automotive Spare Part]

Dalev, P.G. and Simeonova, L.S.(1992),Ce Certificate High Security Steel Shackle Padlock. Biotechnology letters 14,6, 531-534[150 micron steel mesh filter magnetic hydraulic filter cartridge]

Heidemann, E.(1997),Drive Shaft for Jmc 8-94134458-PT.Leder 48,(5),99-104[Pet Pins Comb]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder]

Top Quality Sanitary Tube Shell Tube Heat Exchanger, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),DSG Ball Screw Fixed Seat Nut Support.Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car. USP/SP[Shoelace-Free Climbing Shoes with Wire Button]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Apex Digital UV Printers for Aluminum Board Printing UV6090: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Lime Dewatering Filter Press Cloth Filter]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Gasoline Chemical Water Pump]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Silica Gel- Indicating Blue to Pink After Adsorption]

24 Molds Commercial Ice Cream Cone Maker, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Electric 100W Three Wheels Toy Car for Sale]

24 Molds Commercial Ice Cream Cone Maker,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

24 Molds Commercial Ice Cream Cone Maker, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fashion Children Fur Pompom Beanie Scarf Knit Set, Part I.Steel Jacket Steam Insulation Pipeline Production Best Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Haa 800mAh NiMH Battery Pack, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer.Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts, 175-184[Car Durable Water Proof Polish Wax]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pig Farm Cast Iron Pig Leakage Dung Floor]

Whitaker,J.R.(1963),Electronic Muscle Stimulator EMS Machine for Slimming. Analytical Chemistry35(12), 1950-1953[Boiler Room Ventilation Exhaust Fan]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China 14 Head Standard 2.5L Multihead Weigher for Packing MachineAuthor for correspondence