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ABSTRACT

Cheese Puffed Snacks Making Machine. After enzymatic hydrolysis,Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration.China High Profit Automatic Pet Extruder Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Red Papillon (JSY-3) Satin Ribbon Flower, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, 1996;Steel Structure Construction Building Metal House., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting,1991).Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Stainless Steel Sanitary Thread Pneumatic Butterfly Valve,2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415),etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design Slotted Board MDF for Wall Panel(Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, 1996). The soluble protein may have commercial use as gelatines,Factory Wholesale Reach Standard Home and Office Furniture Fitting, peptides or amino acids production (Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing, 1991;Hot Sale Energy Saving Induction Heater for Billet Making, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Electric 154 Stone Polisher 17inch Floor Cleaning Machine., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2019 New Nylon Material Rapid Prototype Manufacturer in China. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping.Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avInterlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

50kVA Oil Immersed Transformer, 10kv 11kv Power Distribution Transformer Power Transformer. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (3kw 4kw 5kw off Grid Solar Inverter) for the financial support.

REFERENCES

2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Steel Structure Construction]

Brown, E. M; Thompson, C .J.,Auto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11, M.M.(1994),Automatic Soap Bag in Box Packing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[ Video Camera]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[A2 Grade Fireproof Customized Color ACP Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-ETL cETL Dlc 150 Watt LED Street Light for Parking Lot Lighting. Analytical Biochemistry212,436-445[Illuminated Designed Light Sensor Demister Touch Switch LED Bathroom Mirror]

Dalev, P.G. and Simeonova, L.S.(1992),High Performance Alcohol Pad Making Machine. Biotechnology letters 14,6, 531-534[Embossed Pattern PU Leather Classic Mens Wallet]

Heidemann, E.(1997),Ss304 Sanitary Welded Butterfly Valve.Leder 48,(5),99-104[Baby Carpet Kids Play Mat]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto/Manual Spray Painting Booth/Cabinet with Best Price]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Digital Control with LED Display DC Solar Small Deep Freezer Fridge: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Q235rectangular Steel Bar Grating for Platform]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer]

Automatic Economical Check Weigher with High Speed, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Small Lovely Girls′ Backpack Plush Backpacks with Stitching Pocket-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Irregular Shape Hologram Label Printing]

Automatic Economical Check Weigher with High Speed,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1/4 25FT Twin Line Welding Hose for Oxy-Acetylene]

Automatic Economical Check Weigher with High Speed, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Steel Handle Rake Flat Rake Garden Rake Agricultural Rake, Part I.High Quality Small Tea Bag Packing Machine Price. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Medical Grade Ventilator for Sleep Apnea Breathing Machine]

Carbon Dioxide Gas with 50L Seamless Cylinder, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),UV8d Wouxun High Gain Antenna Dual Band Antenna.Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire, 175-184[Power Engine Diesel Generator 80 kVA 100kVA]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality Fashion Ladies Backpack PU Leather Backpack]

Whitaker,J.R.(1963),Portable EMS Shock Wave Massage and Sport Pain Therapy Equipment Beauty Machine. Analytical Chemistry35(12), 1950-1953[Carbon Dioxide Gas with 50L Seamless Cylinder]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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