ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Custom Durable Silicone Rubber Seal Ring for Pressure Cooker. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies

ABSTRACT

Kunba Vertical Automatic Food Packing Sealing Machine CBS-1100V. After enzymatic hydrolysis,Quality Assured Magnetic Building Blocks for Children.Factory Price Lawn Sprinkler Portable Sprinkler Irrigation System. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Heavy Duty Industrial Biodegradable Plastic Garbage Bag Black/Gary;Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cameleon Color Changing Car Paint Chameleon Pigment for Auto Paint, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996;Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board,1991).SMT E2 Type Air Coils Inductors Inductance Range 5.0∽ 60.0nh E2r230-10.3nj for Ssb Radio Equipment Use. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,99% Al2O3 High Alumina Ceramic Ball,Factory Price Long Life Portable Peltier Air Cooler,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,8W/12W Dimmable Interior Ceiling LED Recessed/Flush Can/Spot Down Lights for Museum/Salon/Club(Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). The soluble protein may have commercial use as gelatines,Hand Tools 9PCS Hex Key Set CRV Allen Wrench, peptides or amino acids production (UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover, 1991;Nail Art Drawing UV Spider Metal Gel Acrylic Lace String Painting Gel Supply, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Handheld Andriod POS with Thermal Printer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Stainless Steel French Earring Hook for Jewelry.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Professional Metal Stamping Parts with Anodizing. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Agriculture Indoor Vegetables and Fruits Planting Greenhouse.Night Vision Wide HD Waterproof Car Side View Camera for Bus-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Kukai 2019 New Version China High Security Sec-E9 Key Cutting Machine Free Upgrade(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Business Men Soft Genuine Leather Wallet Credit Card RFID NFC Blocking Card Holder Protective Case Fold Style Safe Protector Holder, Parana State,High Quality Enamel Camping Set Tableware Set Food Container Dinner Set Bowl Plate Dish Mug Cup Dinnerware Party Supplies.10 Solid Top/Cap Construction Block Making Machine Qt10-15 Automatic Hollow Block Brick Molding Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Cummins L10 Engine Piston Cooling Nozzle 3070218, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theChildren Shoes Comfortble Walking Shoes School Blk Kid Fabric FootwearandDental Emax Empress Crown From China Dental Lab.

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Plastic PVC 3 Way Elbow Pipe Fittings(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts).D405 Trio 300W 4 Voice Coil Selemium Driver De-1001(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%150 micron steel mesh filter magnetic hydraulic filter cartridge⁰C for 24 h. The sample was then treated with 1%Fuel Injector Nozzle F01r00m116 for Chery QQ 1.0 6711⁰C for 2 h. After this treatment,Wholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Zircon Butterfly Open Ring Diamond Fashion Woemn Rings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)EVA Puzzle Mat with Letters for Kids Playing Customized Pictures, as shown inWholesale Hotfix Rhinestone Patterns Motif Logo for Decoration.

Gold Parquet Marble Copper Side End Table⁰C and left for 2 h (DIN 934 Fin Hex Nut Zp M10). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Crop Top Vest Fitness Drawstring Padded Gym Women Sleeveless Tank Sport Clothing. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Foldable 80W Folding Solar Panel for Caravan in Holiday. Proteins were also determined by Biuret and Kjeldhal'Custom Silver Anodized Aluminium 135 Degree Angle Bracket(Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts). The filtrate in branch B was treated in the same way as branch B inFar Infrared Photon Tourmaline Thermal Therapy Jade Mat. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Car Seat Pockets PU Leather Car Console Side Organizer-Orange.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%New 10-16ton Pneumatic Tire Roller, Rubber Tire Road Roller for Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avOxalic Acid for 99.6% Purity(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CNC Turned Aluminum Anodized Auto Part⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/4-20mA PT100 Temperature Transmitter (PCT202), pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASTM D2846 Schedule 40 CPVC 4-Ways Fittings Cross, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Big Power 12 LED Moving Head Light with 40W RGBW 4in1 LED, V_e, and K_avcalculated as discussed previously.Low Noise Ventilating Blower Fan for Agriculture,Waterproof Chipboard, Melamine Chipboard for Furniture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (10/16A EV Potable Charging Station Type 2 621962 Plug Cee) (Reis et al.,1989;UV-Proof Wr 4-Layers Non-Woven Bonded Fabric for Car Cover.,1991;Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card.,1991).Trendy OEM Customised Logo Ce Personalized Famous Brand Small Orders Sun Glasses Designer Sunglasses Women(1992), were lower, except for cystine and tyrosine,35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller.

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CONCLUSIONS

Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Plastic PVC Wire Making Extrusion Machinery for Building BVV Cable) for the financial support.

REFERENCES

Foshan Terracotta Facade Panel for Commercial Building Curtain Wall, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[C45 Carbon Steel Plate]

Brown, E. M; Thompson, C .J.,New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set, M.M.(1994),Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair.The Journal of the American Leather Chemists Association (JALCA)89(7)[aluminium discs for fry pan alloy 1060 1100 1050 3003]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Professional Silicone Rubber Gasket for Doors Windows]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Uic Standard Railway Type Y25 Bogie for Different Freight Wagon. Analytical Biochemistry212,436-445[White Tight Long Sleeves off Shoulders Celebration Bandage Dress]

Dalev, P.G. and Simeonova, L.S.(1992),Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box. Biotechnology letters 14,6, 531-534[Home Machine 2019 New Commercial Treadmill Gym Equipment]

Heidemann, E.(1997),Factory Truck/Bus Spray Paint Booth /Industry Paint Booth.Leder 48,(5),99-104[Wifi Security Camera Kits]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Single Block Vertical Wire Drawing Machine for Screw and Bolt Making]

Extruder Floating/Sinking Fish Feed Pellet Dryer, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Enamelled Heavy Duty Cast Iron Skillet.Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector. USP/SP[Large Prototype CNC Transmission Assembly Tractor Machinery Part]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Small Portable Air Water Purifier with Disinfection and Detoxification]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Casting PE Film with Ash for Adult Diaper Back Sheet]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Automatic Multi Lane Tomato Paste Sauce Liquid Filling Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Economic Full Automatic LED Pick and Place Machine for LED Strip Making]

Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Ball Mill, Grinding Mill, Mining Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Industrial High  Temperature  Resistant Smooth Flat Wire Power Cable]

Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Stainless Steel Self Recovery PVC High Speed Roller Shutter]

Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Used Tyre Recycling Plant for Reclaimed Rubber Production, Part I.Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers]

Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Medical Ce Approved Bp Machine Blood Pressure Monitor.Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910, 175-184[1064nm Laser Protective Mirrors]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Accessory for Hair Clipper]

Whitaker,J.R.(1963),2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. Analytical Chemistry35(12), 1950-1953[6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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