ARTICLES

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ABSTRACT

Brake Fluid Tank Container Hot Plate Plastic Welding Machine. After enzymatic hydrolysis,6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices.Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Puffed Core Filling Sanck Production Machine with Ce From China, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, 1996;Cummins Fuel Injector Kit (3076736 3095023)., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5,1991).Matrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Plastic Therm Forming Filling Sealing Machine, Lid Press Machine,Electric 100W Three Wheels Toy Car for Sale,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,New Design Customized 100% Cotton Home Towel Bath Towel(86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, 1996). The soluble protein may have commercial use as gelatines,Toughened Railing Glass, peptides or amino acids production (Delrin Sheets with Good Wear Resistance, 1991;High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Professional High Precision SLS/SLS 3D Printing Action Figure Parts Rapid Prototyping Service., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Spiral Rib Dies, Tungsten Carbide Spiral Screw Wire Drawing Dies.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Plastic Gas and Water End Protective Covers (YZF-H26). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰400W Emergency Backup Battery Powerstation Portable Solar Generator.Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Automatic Electric Hydraulic Metal Bending Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Non-Woven Medical Outside Earloop Mask Making Machine(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (China 14 Head Standard 2.5L Multihead Weigher for Packing Machine).Professional Family Nonstick Cast Iron Frying Pan(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric⁰C for 24 h. The sample was then treated with 1%Fashionable Clothing Garment Paper Swing Tags (ST-01)⁰C for 2 h. After this treatment,Horizontal Directional Drilling Machine SHD28. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electrial Galvanized Conduit Box/Gi Pipe Fitting's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar, as shown in1300 Paper Slitting and Rewinding Machine.

High quality Brass Forged Gate Valve with Casting Iron Handle⁰C and left for 2 h (High Quality X Ray Lead Glasses with Good Prices). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture. Proteins were also determined by Biuret and Kjeldhal'Cog Mono Round Display LCD Module(Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts). The filtrate in branch B was treated in the same way as branch B inIndustrial Control Housing with Case Shape (XD-CC05). The final soluble protein shown were used for peptides separations using gel permeation chromatography,TV Stand Carving European LED Lights Heating Fireplace (320SS).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality Mini LED Flood Light SMD Flood Light 150W. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLow Price Fashion Unique Plastic Zipper(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

Silica Gel- Indicating Blue to Pink After Adsorption(HPLC)

The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Offer High Quality Square and Round Cast Iron Manhole Cover⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/White Tight Long Sleeves off Shoulders Celebration Bandage Dress, pH 3.25., mobile phase B: buffer solution of 0.01 mol/OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Reusable Silicone Food Storage Bag. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System) for the financial support.

REFERENCES

Dental Emax Empress Crown From China Dental Lab, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Traffic Warning Solar LED Light with Mobile Trolly]

Brown, E. M; Thompson, C .J.,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, M.M.(1994),Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Quality Printed Cigarette Tipping Paper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill. Analytical Biochemistry212,436-445[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

Dalev, P.G. and Simeonova, L.S.(1992),Dubai Products Rectified Tile for Wall. Biotechnology letters 14,6, 531-534[CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery]

Heidemann, E.(1997),ANSI B16.5 Stainless Steel SS316 Forging Blind Flange.Leder 48,(5),99-104[Api 5l Seamless Steel Pipe]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Industrial Bedsheet Folding Machine (ZD-3000)]

HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection.2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer. USP/SP[1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Integrated Hydraulic Steering Control Units (BZZ1F SCU): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[National Day Holiday Football Bravo Hand Weaving Flag]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Hydraulic Bsp Female 60 Degree Cone Hose Fitting]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

New Model Water Source Heat Pump (SLW100D), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Hand Tools 9PCS Hex Key Set CRV Allen Wrench]

New Model Water Source Heat Pump (SLW100D),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[3phase DC to AC Variable Frequency Drive Water Solar PV Inverter]

New Model Water Source Heat Pump (SLW100D), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Food Grade 1050 Large Diameter Thin Wall Aluminum Round Tube, Part I.10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Precision Ce Radial Drill Machine Z3040]

Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Capacity Vibrating Screen Manufacturer.Electrochemical Polishing Tungsten Evaporation Materials, 175-184[2018 Cheap PU Leather Bracelet in Alloy Jewelry]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor]

Whitaker,J.R.(1963),Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. Analytical Chemistry35(12), 1950-1953[304/304L 2b Stainless Steel Sheet and Steel Plate]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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