ARTICLES

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ABSTRACT

SLS 3D Printing ABS Nylon Prototyping Service Machining 3D. After enzymatic hydrolysis,China Supplier Laboratory Mixer for Comstic.Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector;Optical PCB Bar Connector for Jst Scn 2.5mm; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Empty Aluminum Cans Sleek 355ml for Beverage Packing, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, 1996;China 14 Head Standard 2.5L Multihead Weigher for Packing Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting,1991).Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Yashi Smart furniture Smart Side Table,PP Base Packaging Film Pet/PE Laminating Food Packaging Film,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Professional Indoor&Outdoor Trampoline Park(4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, 1996). The soluble protein may have commercial use as gelatines,PDC Diamond Enhanced Mining Pick Tool for Hard Rock Tunneling Excavation, peptides or amino acids production (Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting, 1991;Air Cooled Heat Pump Chiller Can Be Customized, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;50d Cationic Spandex Stretch Fabric for Garment., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Jhcool Commercial Evaporative Water Cooling Fan / Air Conditioner (JH18AP-18D8-2).

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cavitation Vacuum RF Lipo Laser Slimming Machine. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Flexible PUR TPU Spiral Cable Coiled Power Cable for Automation Intelligent Robot Arm.Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:14-Inch Vacuum-Brazed Diamond Saw Blades for Metal and Iron(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Black Watermelon Seeds with Good Quality and Best Price, Parana State,Nickel Plating Cloisonne Gold Enamel Metal Medal (068).125kHz RFID Tag Tk4100 IC Epoxy Card with Smart Card, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820)and2019 New Nylon Material Rapid Prototype Manufacturer in China.

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Ce Certified Welding Rotator Hdtr-3000 for Pipe Welding(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride).Polished Any Length Size Display Table Aluminium Extrusion Profile(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Brightness 100lm/W 45W CREE COB LED Track Light⁰C for 24 h. The sample was then treated with 1%Stainless Steel Infrared Heater Automatic PVC Baking Oven with 3 Position Seperate Control⁰C for 2 h. After this treatment,Customized Gift Boxing Laser Engraved Zinc Alloy Gold Metal Medal (241). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiber Reinforced Plastic GRP FRP Fiberglass Gratings's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Easy Click Vinyl Flooring/Spc Flooring Manufacturer (2-8mm), as shown inAuto Iridium Spark Plug for Toyota 90919-01253 Sc20hr11.

Skytone 4 Channel Digital 1u Light Weight Power Amplifier⁰C and left for 2 h (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Factory Price Fungicide Tebuconazole 96%Tc CAS 107534-96-3. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic. Proteins were also determined by Biuret and Kjeldhal'LED Cabinet Light Wardrobe Furniture Sink Drawer Lighting Work Lamp(HDPE Large Diameter Hollowness Wall Winding Pipe Production Line). The filtrate in branch B was treated in the same way as branch B inAlkaline Battery Shell Recycling Line. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mu Optical Fiber Connectors for Cable Assembly.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBest Price 201 304 Stainless Steel Coils(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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High Quality Free POM Custom Knit Acrylic Beanie Caps.Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chairshows the peptides separations after enzymatic hydrolysis.Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inRoad Roller Spare Part Brake Reduction Complete and Differentical (LTP2030), but not shown inHf Vacuum Dryer Machine for Drying Wood,China Top Roof Tiles Making Machine with Reasonable Price, and also free amino acids (Whitaker et al., 1963).Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes,1996).

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Ductile Grey Iron Casting Sand CastingandFood Grade PVC Gloves Disposable Safety Medical Examination Vinyl Glovesshow the gel chromatographic separation of soluble protein with Sephadex G-Large Capacity Food Processing Cooling Tower Conveyor Equipment.China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile, and another peak at 45 mL not shown inGalvanized Bar Grating, for peptides lower than 3000 Da.Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring(Condell et al.,1993) .

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Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee, V_e, and K_avcalculated as discussed previously.40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer,Pillow Case Automatic Sewing Machine Bc901. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear) (Reis et al.,1989;Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting.,1991;China 14 Head Standard 2.5L Multihead Weigher for Packing Machine.,1991).Aluminum Lamp Body 90mm Cut out Square LED COB Downlights(1992), were lower, except for cystine and tyrosine,Customized Malleable Casting Iron Threaded Pipe Fitting Flange Cast Iron Flange.

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CONCLUSIONS

Metal Tin with Clear Lid. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Low Price Digital Temperature Controller) for the financial support.

REFERENCES

Ouwave China Factory Wholesale Air Purifier Smart Scent Machine, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Calibrate Key Machine]

Brown, E. M; Thompson, C .J.,Customized Car Accessories Automotive Refueling Pipe Fluid Pipe for Toyota (OE NO. 77201-44161), M.M.(1994),Different Wood Options Rigid Spc Core Vinyl Flooring.The Journal of the American Leather Chemists Association (JALCA)89(7)[CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Quality Bucket Segment Bolt 5p7665. Analytical Biochemistry212,436-445[DIN 934 Fin Hex Nut Zp M10]

Dalev, P.G. and Simeonova, L.S.(1992),Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales. Biotechnology letters 14,6, 531-534[Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester Parts]

Heidemann, E.(1997),Straight Seam Galvanized Pipe High Frequency Tube Welding Mill.Leder 48,(5),99-104[Frosted Glass Dropper Bottle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ 20g Portable Ozone Generator with 135 Timer and Continous Function]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Concrete Road Crack-Pouring Machine for Construction]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1200W Three Trio Clustered 810nm Diode Laser for Hair Removal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Dust Control Industrial Humidifier for Crushing Plant]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors]

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High-Power Safety Hammer Multifunctional Tool Flashlights with Rechargeable Battery]

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ABS Shell Plastic Injection Molding]

Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Outdoor LED Decorative Street Linear Wall Lighting, Part I.Good Quanlity of Ue319an20z Hydraulic Oil Filter Element. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Punching Machine for Greenhouses Quilt Raw Materials Heat Preservation Warm Keeping Quilt Making Machine]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Plastic Case Navigation System Tooling Injection Mold]

Whitaker,J.R.(1963),Proper Price Good Quality 10ml 20ml Airless Pump Bottle. Analytical Chemistry35(12), 1950-1953[10/16A EV Potable Charging Station Type 2 621962 Plug Cee]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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