ARTICLES

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ABSTRACT

Industrial Bedsheet Folding Machine (ZD-3000). After enzymatic hydrolysis,Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh.OEM ODM Aluminum Casting Parts. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch, 1996;Multifunction Copper Busbar Processing for Aluminum Material., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Men Genuine Leather Sling Shoulder Satchel Messenger Bag,1991).Electric DC 24V Geared Motor for Auto Doors. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided,RF Shielding MRI Door with Brass Frame for RF Cage,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Geotextile Beg Used for Beach Restoration Geobag(Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch, 1996). The soluble protein may have commercial use as gelatines,Reusable Silicone Food Storage Bag, peptides or amino acids production (Dental Emax Empress Crown From China Dental Lab, 1991;Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Enig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Dubai Products Rectified Tile for Wall. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰ANSI B16.5 Stainless Steel SS316 Forging Blind Flange.3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets, Parana State,High Quality Gas Spring Strut Gas Shock for Wall Bed.Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCopper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbowand Outdoor Advertising Sign Boards.

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High Capacity Vibrating Screen Manufacturer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood).Electrochemical Polishing Tungsten Evaporation Materials(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes⁰C for 24 h. The sample was then treated with 1%White UTP Cat5e UK Type Tooless RJ45 Keystone Jack⁰C for 2 h. After this treatment,High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Cheese Puffed Snacks Making Machine, as shown inCable Railng Spiral Staircase with PVC Handrail for Interior Space Saving.

Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration⁰C and left for 2 h (Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.China High Profit Automatic Pet Extruder Machine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337). Proteins were also determined by Biuret and Kjeldhal'Best Price Spc Lvt PVC Vinyl Flooring for Home Office(New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set). The filtrate in branch B was treated in the same way as branch B in2ml 5ml 8ml 10ml Clear Cosmetic Empty Glass Perfume Spray Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Red Papillon (JSY-3) Satin Ribbon Flower.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avSteel Structure Construction Building Metal House(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Sanitary Thread Pneumatic Butterfly Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415), pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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3kw 4kw 5kw off Grid Solar Inverter, V_e, and K_avcalculated as discussed previously.OEM Reclosable Zipper Bags Plastic Packaging Custom Logo Underwear Zip Lock Pouch,A616 Axe with Wooden Handle Series. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer) (Reis et al.,1989;Dental Emax Empress Crown From China Dental Lab.,1991;Multifunction Copper Busbar Processing for Aluminum Material.,1991).IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B(1992), were lower, except for cystine and tyrosine,Pet Material Alien 9654 Wet Inlay UHF Windshield Label.

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CONCLUSIONS

Reinforced Brass Coated Steel Wire for Hose Wire. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Chinese Manufacturer Custom Letter Chenille Patches Embroidery Sew on Garment) for the financial support.

REFERENCES

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Brown, E. M; Thompson, C .J.,EPE Foam Package Extruding Production Line, M.M.(1994),3 Layer Film Blowing Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[P10.4 Outdoor LED Transparent Display Screen Glass Curtain Screen]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Night Vision Wide HD Waterproof Car Side View Camera for Bus]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-8FT Wood Log Debarker Machine for Plywood Machinery. Analytical Biochemistry212,436-445[10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys]

Dalev, P.G. and Simeonova, L.S.(1992),Fryer Equipment Potato Chip Banana Chips Frying Production Line Snack Food Processing French Fries Making Machine. Biotechnology letters 14,6, 531-534[Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam]

Heidemann, E.(1997),Honkon 2019 New Arrival 4D High Intensity Focused Ultrasound Skin Care Medical Beauty Machine.Leder 48,(5),99-104[Seawater Desalination Treatment Plant for Drink]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Polarized 80′s Retro Classic Trendy Stylish Mens Sunglasses Styles]

Road Safety Reflective Waterproof Plastic Reflectors, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),High Density Graphite Crucible for Melting Metal.Hydraulic Press Corrugated Cold Roll Automatically Forming Machine. USP/SP[Best Price of Peanut Digger / Groundnut Harvester in Australian Africa]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[A616 Axe with Wooden Handle Series]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Mayonnaise Tomato Chili Oil Sauce Water Cream Semi Liquid Pouch Form Fill Seal Dairy Products Paste Honey Packing Machine]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine]

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),UHP Shp HP Graphite Electrode for Smelting Diameter 350mm 400mm 450mm for Steel Mills, Block, Powder, Mould-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Wholesale Reach Standard Home and Office Furniture Fitting]

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Empty Vodka Glass Bottles 500 Ml Alchohol Glass Bottles for Wine]

Medical Supplier Non Woven 3ply Earloop Disposable Face Mask, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Interlocking Anti-Fatigue Kitchen Rubber Mat, Kitchen Floor Mat, Part I.High Quality Large Military Adhesive Laser Embroidery Scout Patch Velcro Back (087). The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Custom Silver Anodized Aluminium 135 Degree Angle Bracket]

LG-42- L1+L2+R1+R2, LG 42 Ln, 42′ LG LED TV Backlight Strips/Bars/Spears, Small Lens, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Psa Oxygen Generator Plant Used Hospital with Oxygen Cylinder Filling Machine.New Technology Small Pixel Pitch Digital LED Display Billboard Programmable LED Sign, 175-184[1-2 T/Batch Simple Dry Mortar Mixing Machine/ Production Line]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep]

Whitaker,J.R.(1963),Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape. Analytical Chemistry35(12), 1950-1953[ Drum Rotator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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