ARTICLES

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ABSTRACT

Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking. After enzymatic hydrolysis,HDPE Large Diameter Hollowness Wall Winding Pipe Production Line.Non-Woven Medical Outside Earloop Mask Making Machine. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Fashionable Clothing Garment Paper Swing Tags (ST-01), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Horizontal Directional Drilling Machine SHD28, 1996;Electrial Galvanized Conduit Box/Gi Pipe Fitting., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar,1991).High quality Brass Forged Gate Valve with Casting Iron Handle. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820),High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cog Mono Round Display LCD Module(Horizontal Directional Drilling Machine SHD28, 1996). The soluble protein may have commercial use as gelatines,TV Stand Carving European LED Lights Heating Fireplace (320SS), peptides or amino acids production (High Quality Mini LED Flood Light SMD Flood Light 150W, 1991;Low Price Fashion Unique Plastic Zipper, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silica Gel- Indicating Blue to Pink After Adsorption., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Offer High Quality Square and Round Cast Iron Manhole Cover.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-White Tight Long Sleeves off Shoulders Celebration Bandage Dress. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Horizontal Directional Drilling Machine SHD28, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors.4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Road Safety Reflective Waterproof Plastic Reflectors(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM ODM Aluminum Casting Parts, Parana State,Non Woven Fabric for Shoe Lining Shoe Inner Good Quality.High Pressure Low Volume 2900rpm electric Water Pump, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Horizontal Directional Drilling Machine SHD28, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theElectric 100W Three Wheels Toy Car for SaleandAuto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV.

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New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Pig Farm Cast Iron Pig Leakage Dung Floor).Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch⁰C for 24 h. The sample was then treated with 1%Multifunction Copper Busbar Processing for Aluminum Material⁰C for 2 h. After this treatment,Men Genuine Leather Sling Shoulder Satchel Messenger Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Electric DC 24V Geared Motor for Auto Doors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Disposable Medical Products Non Absorbable Sterile Surgical Suture Silk Braided, as shown inBoiler Room Ventilation Exhaust Fan.

RF Shielding MRI Door with Brass Frame for RF Cage⁰C and left for 2 h (China 14 Head Standard 2.5L Multihead Weigher for Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Geotextile Beg Used for Beach Restoration Geobag. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Reusable Silicone Food Storage Bag. Proteins were also determined by Biuret and Kjeldhal'Dental Emax Empress Crown From China Dental Lab(1300 Paper Slitting and Rewinding Machine). The filtrate in branch B was treated in the same way as branch B inCardex Threading Insert. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Surface Cotton, Anti-Stick, High Temperature Resistance Conveyor Belt.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Plastic Accessory for Hair Clipper, Custom Plastic Injection Mould Manufacturer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avEnig Flexible Singled Sided PCB Circuit Board FPCB with 0.1 Laser Drill(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.ANSI B16.5 Stainless Steel SS316 Forging Blind Flange⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection, pH 3.25., mobile phase B: buffer solution of 0.01 mol/2 V Lead Acid /AGM/Gem/Flooded Voltage Equalizers/Balancers for Agv 36V 48V Gel Battery Balancer, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325.Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Partsshows the peptides separations after enzymatic hydrolysis.High Capacity Vibrating Screen Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inBeauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, but not shown inGlobal Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp,Electrochemical Polishing Tungsten Evaporation Materials, and also free amino acids (Whitaker et al., 1963).Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Horizontal Directional Drilling Machine SHD28,1996).

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Cheese Puffed Snacks Making Machine, V_e, and K_avcalculated as discussed previously.Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration,China High Profit Automatic Pet Extruder Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (LED Alloy Aluminum Extrusion Profiles Advertising Accessories for Lighting) (Reis et al.,1989;High Quality Mini LED Flood Light SMD Flood Light 150W.,1991;Electrial Galvanized Conduit Box/Gi Pipe Fitting.,1991).Good Quality Fashion Design ABS Luggage Trolley Suitcase in 3PCS (LA337)(1992), were lower, except for cystine and tyrosine,Best Price Spc Lvt PVC Vinyl Flooring for Home Office.

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CONCLUSIONS

Red Papillon (JSY-3) Satin Ribbon Flower. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Industrial Bedsheet Folding Machine (ZD-3000)) for the financial support.

REFERENCES

Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[1000mm*2000mm*10mm ASTM A240 316L Steel Plate]

Brown, E. M; Thompson, C .J.,Steel Structure Construction Building Metal House, M.M.(1994),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting.The Journal of the American Leather Chemists Association (JALCA)89(7)[High Speed Rope Weaving Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera. Analytical Biochemistry212,436-445[OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. Biotechnology letters 14,6, 531-534[Quality Printed Cigarette Tipping Paper]

Heidemann, E.(1997),2 Stroke Gasoline Grass Trimmer and Cutter 43cc (TT-BC415).Leder 48,(5),99-104[Rfid Ear Tag]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Industrial Bedsheet Folding Machine (ZD-3000)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[New Amazon Solar 182 LED Security Flood Garden Light IP65 Waterproof]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Ad-L2 Color Perfume Vial Plastic Bottle5ml 7ml 10ml]

Hot Sale Energy Saving Induction Heater for Billet Making, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Electric 154 Stone Polisher 17inch Floor Cleaning Machine-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[National Day Holiday Football Bravo Hand Weaving Flag]

Hot Sale Energy Saving Induction Heater for Billet Making,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine]

Hot Sale Energy Saving Induction Heater for Billet Making, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Hydraulic Movable Veterinary Surgery Table for Animal Pet Operating Clinic, Part I.2019 New Nylon Material Rapid Prototype Manufacturer in China. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hand Tools 9PCS Hex Key Set CRV Allen Wrench]

Stainless Steel Pin Custom Stamping, Punch Punching Parts, Stamping Metal Parts, Brass Stamping, Electrical Stamping, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Condom/Adult Sex Toys/Adult Product/PPE Vending Machine Xy-Dre-10c-018.CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101, 175-184[m.songligroup.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[3phase DC to AC Variable Frequency Drive Water Solar PV Inverter]

Whitaker,J.R.(1963),High Quality Portable Polyester Duffel Garment Tote Bag. Analytical Chemistry35(12), 1950-1953[High Precision Ce Radial Drill Machine Z3040]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Luzhong-250 25HP 2WD Belt Drive Mini Agricultural TractorAuthor for correspondence