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ABSTRACT

Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator. After enzymatic hydrolysis,High Temperature & IR Drying Electronic Oven / Furnace.Ce Approved Bowrider Leisure Boat for 6-8 People. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Industrial Original Cheap Brown Kraft Paper Core for Printer 0.5inches * 220mm, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Concrete Electric Pole Pile Making Spinning Machine, 1996;Shop Front Indoor Durable Hanging Banner Advertising Signs., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Cosmetic & Food Grade Sodium Hyaluronate,1991).High Frequency Circular Vibrating Screen. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fiberglass/Composite Mesh for Walkway FRP Grating,High Quality 33kv 6.3kv 1000 kVA 50% Copper and 50% Aluminum Material Distribution Transformer Made in China,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine(Concrete Electric Pole Pile Making Spinning Machine, 1996). The soluble protein may have commercial use as gelatines,Union NPT Bsp BSPT Stainless Steel Pipe Fittings, peptides or amino acids production (High Brightness IP67 Red Color SMD5050 Chip 60leds 14.4W DC12V LED Strip, 1991;Marine Anemometer / Wind Meter/ Anemoscope with Alarm Function for Vessel/Ship, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Tear Strip Tape.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Healthy Grooming Kit for Caring Newborn Baby. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Concrete Electric Pole Pile Making Spinning Machine, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Reusable Stainless Steel Metal Collapsible Portable Foldable Telescopic Straw with Silicone Tips Cleaning Brush.Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Private Label Fitness Wear Sublimation Printing Cheap Long Womens High Waist Yoga Pants Custom(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (304 Stainless Steel Expansion Anchor Bolt).Stainless Steel LED Underground Light, Ground Lighting IP66(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets)⁰C for 24 h. The sample was then treated with 1%K Series Helical Bevel Reduction Gearbox Motor⁰C for 2 h. After this treatment,OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Mini Outdoor Hydraulic Man Aluminum Lift, as shown in2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Frequency Online UPS W/3W-20K. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avLuxurious Fire and Burglary Proof Hidden Hotel Security Safe Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.14 Head Multihead Weigher for Pork with Screw Feeding⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve, pH 3.25., mobile phase B: buffer solution of 0.01 mol/PSF 1000 Flat Plate Upper Discharge separator, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Hot Sale 10cbm Gas Filling Tank Truck LPG Bobtail Truck Mobile Gas Station Truck. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality Aluminum Street Light Pole) for the financial support.

REFERENCES

China OEM Alloy Steel Precision Casting, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Indoor Basketball Court]

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Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Square Vibrating Sieve Machine]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor. Analytical Biochemistry212,436-445[Optical PCB Bar Connector for Jst Scn 2.5mm]

Dalev, P.G. and Simeonova, L.S.(1992),Galvanized Metal Welded PVC Coated Wire Mesh Fence. Biotechnology letters 14,6, 531-534[Glazed Metal Roof Tile Roll Forming Machine with Ce]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Pet Bottle Washing Recycling Line in China]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SLS 3D Printing ABS Nylon Prototyping Service Machining 3D]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[m.joinhome-sales.com]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price]

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Top Quality Sanitary Tube Shell Tube Heat Exchanger,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Kindergarten Playground Preschool Kids Plastic Slide and Swing]

Top Quality Sanitary Tube Shell Tube Heat Exchanger, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c, Part I.High Precision iPhone Cases Fiber Laser Marking Machine. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2-25mm Co-Extruded Highlight PVC Foam Board]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box]

Whitaker,J.R.(1963),HDPE Pipe for Water Supply (OD: 16mm-800mm). Analytical Chemistry35(12), 1950-1953[Household Use Food Packing 8011 Aluminum Foil]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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