ARTICLES

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ABSTRACT

Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. After enzymatic hydrolysis,Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids.Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair;Single Block Vertical Wire Drawing Machine for Screw and Bolt Making; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (China Electric PCB Circuit Board with High Quality, 1996;OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Anodized Perforated 3D Facade Windows Screen for Commercial Building,1991).Full Cream Milk Powder Filling Machine (XFF-G). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.,Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale(China Electric PCB Circuit Board with High Quality, 1996). The soluble protein may have commercial use as gelatines,Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, peptides or amino acids production (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment, 1991;PVC Car Seat Belt Shoulder Protector 80438, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Professional Full Automatic Machine for Making Wood Pellets.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-High Quality 20PCS Colorful Makeup Brush Set. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (China Electric PCB Circuit Board with High Quality, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy.PE Plastic Road Safety Barricade Caution Tape-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Screw Barrel for PVC PPR Pipe Extrusion(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

FTTH Field Sc Upc Fiber Optic Fast Connector for Drop Cable, Parana State,Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression.95g 3D Tooth Stain Remover Whitelock Toothpaste, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (China Electric PCB Circuit Board with High Quality, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theHemp Recycle Polyester Blenched Jersey Knit FabricandColor Coated Prepainted PPGI PPGL Steel Sheet.

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Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Mini Succulent Planters).Al/Pet/Al Double Sides for Aluminium Flexible Air Duct(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%European Standards 100A 24V Electrical Relay for Green Energy⁰C for 24 h. The sample was then treated with 1%Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal⁰C for 2 h. After this treatment,Canvas Shoulder Weekend Shopping Big Bag. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Visuable Reflective Tape for Vehicle Road Safety's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Made in China Aluminum Extrusion Window and Door Profile, as shown inCirculating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avDurable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Experienced Construction Steel Structure Building Design⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Heavy Duty 16000L High Pressure Sewer Cleaning Truck, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Low Price Digital Temperature ControllerandNon Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam. Enzymatic (Portable Ink Date Printing Machine for Plastic Bag)High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0(sulfuric acid)High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz(China Electric PCB Circuit Board with High Quality, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods, flow rate of 0.1 mL/min., and l = 620 nm.

Best Items Gh/Genatropin with Naked Vial.Motar Plastering Machine with Screw Pumpshows the peptides separations after enzymatic hydrolysis.Custom Size Gel Nail Polish Set Boxes with Sponge(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSquare Vibrating Sieve Machine, but not shown inOptical PCB Bar Connector for Jst Scn 2.5mm,High Precision Ce Radial Drill Machine Z3040, and also free amino acids (Whitaker et al., 1963).Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (China Electric PCB Circuit Board with High Quality,1996).

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Hot-Sale Products GRP Tower for New Energy Industry, V_e, and K_avcalculated as discussed previously.Fgg 2b 304 Metal Circular Push Pull Connector,Thick Wall Large Diameter Aluminium Tube Manufacture. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Gaogong Group Gypsum Rotary Dryer From Manufacturer Competitive Price) (Reis et al.,1989;Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment.,1991;OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts.,1991).Portable Hydraulic Power Pack 12V Hydraulic Power Unit(1992), were lower, except for cystine and tyrosine,China Supplier Jic Female Eaton Standard Hydraulic Rubber Hose One Piece Fitting 16711RW.

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CONCLUSIONS

52cc Petrol Brush Cutter for DIY Garden Care. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor) for the financial support.

REFERENCES

Anti-Theft EAS Hard Tag Tag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Cotton Sweatbands]

Brown, E. M; Thompson, C .J.,Foldable Silicone Vegtable Fruit Colander Strainer, M.M.(1994),Hans Custom Manufactured Smoothness Zipper Waterproof.The Journal of the American Leather Chemists Association (JALCA)89(7)[2-25mm Co-Extruded Highlight PVC Foam Board]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Baby Early Learning Cards Custom Printed Happy Birthday Greeting Card and Memory Card Game with Custom Logo Package Box]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Quartz Liquid Powder Feeding Torch. Analytical Biochemistry212,436-445[Household Use Food Packing 8011 Aluminum Foil]

Dalev, P.G. and Simeonova, L.S.(1992),Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail. Biotechnology letters 14,6, 531-534[10 X 10 Canopy Pop up Tent Outdoor Gazebo Tent for Exhibition]

Heidemann, E.(1997),Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing.Leder 48,(5),99-104[Fresh Soba Noodles Japanese Noodle]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[3.0inch 1080P HD Car Driving Recorder with Loop Recording WDR Motion Detection]

CNC Aluminum Laser Milling Machinery, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Outdoor P5mm Programmable Bus LED Display Sign.AGM Separator for AGM Battery. USP/SP[Dubai Products Rectified Tile for Wall]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[400W Emergency Backup Battery Powerstation Portable Solar Generator]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Electric Battery Flameless LED Tea Light: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Car Interior Accessories Customized Car Seat Cover]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Production CNC Machining CNC Lathe Machine Parts Machining Stainless Steel]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),CE Certificated Wheel Loader (HQ912) with Agricultural Tire-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Factory Professional Design Wing Shape Engraved Car Label Emblem]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Microsynthetic Fiber Virgin Homopolymer Polypropylene Monofilament Fibermesh]

42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays, Part I.Cosmetic & Personal Care Grade Xanthan Gum. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask]

Professiona Ozone Generator for Swimming Pool, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Precision Plastic Mold Manufacturer in China.Fiberglass Animal Poultry Insulation Grain Tower, 175-184[Sports Backpack Bag]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Luxury Rainfalls Shower Set]

Whitaker,J.R.(1963),Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. Analytical Chemistry35(12), 1950-1953[Injection Mold Maker for Plastic Fighter Toy]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Aluminum Window System Thermal Break Strip (HK Stype35.3mm)Author for correspondence