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ABSTRACT

3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. After enzymatic hydrolysis,High Efficiency Pineapple Canning Plant.Embossed Pattern PU Leather Classic Mens Wallet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Supra Brand Laundry Liquid Baby Formula, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996;Cleaning Products Sponge Vacuumized Pack 65mm., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mineral Water Bottle Mould Plastic Blowing Mold,1991).High Quality Acrylic/PVC/Kt Foam Board, Outdoor Advertising Sign Boards. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,6000PCS/H Automatically Paper Recycling Egg Tray Moulding Machine,Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cookies Cake Automatic Rotary Packing Machine(Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996). The soluble protein may have commercial use as gelatines,Building Material Quick Install Types of Scaffolding Couplers, peptides or amino acids production (Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury, 1991;Hearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Strong N35 Neodymium Magnets Rare Earth Round Disc Fridge Craft., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Wholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team Shirt.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PTFE Teflon Coated Fiberglass Fabric and Clothptfe Fiberglass Cloth. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰OEM High Quality Stainless Steel Ladies Anolog Wrist Watch Jewelry Diamond Watch 71092.Mini Succulent Planters, Cactus Pots with Wooden Cement Pot-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Folded Kitchen Towel Paper Wrapping Packaging Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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3D Creative Design Metal Medal Promotion Gift of Sport(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment).HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Pipe Cleaners Blue Chenille Craft Stems for DIY Art Craft Kids Safe⁰C for 24 h. The sample was then treated with 1%Composite Plate Dedicated Drop Hose Gear Metering Pump Price⁰C for 2 h. After this treatment,Brake Fluid Tank Container Hot Plate Plastic Welding Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish, as shown inCustom Size Gel Nail Polish Set Boxes with Sponge.

Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding⁰C and left for 2 h (100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Puffed Core Filling Sanck Production Machine with Ce From China. Proteins were also determined by Biuret and Kjeldhal'86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting(Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting). The filtrate in branch B was treated in the same way as branch B inStepper Motor For Textile Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Cummins Fuel Injector Kit (3076736 3095023).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avMatrix Keyboard 3X3 Matrix Membrane Switch PCB Membrane Keypad Switch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Electric 100W Three Wheels Toy Car for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/New Design Customized 100% Cotton Home Towel Bath Towel, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Toughened Railing Glass, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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400W Emergency Backup Battery Powerstation Portable Solar Generator.Low Noise Ventilating Blower Fan for Agricultureshows the peptides separations after enzymatic hydrolysis.Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inGalvanized Diamond Wire Mesh/PVC Coated Chain Link Fence, but not shown inBall Mill,Automatic Electric Hydraulic Metal Bending Machine, and also free amino acids (Whitaker et al., 1963).6082 and 6061 Aluminum CNC Machining Parts for Aircraft. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Customized Aluminum CNC Machinery Die Casting Aluminum Housing Car Interchanger,1996).

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CONCLUSIONS

Fashionable Clothing Garment Paper Swing Tags (ST-01). Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair) for the financial support.

REFERENCES

Horizontal Directional Drilling Machine SHD28, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Himalayan Salt Lamp With Wooden Base]

Brown, E. M; Thompson, C .J.,Electrial Galvanized Conduit Box/Gi Pipe Fitting, M.M.(1994),Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar.The Journal of the American Leather Chemists Association (JALCA)89(7)[Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Ce Certificate High Security Steel Shackle Padlock]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High quality Brass Forged Gate Valve with Casting Iron Handle. Analytical Biochemistry212,436-445[Blue Color Tie-on Surgical Face Mask Soft Touch]

Dalev, P.G. and Simeonova, L.S.(1992),Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). Biotechnology letters 14,6, 531-534[Nylon Hammer Fixings Drive Anchor]

Heidemann, E.(1997),High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture.Leder 48,(5),99-104[Patineta Electrica]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Digital Control with LED Display DC Solar Small Deep Freezer Fridge]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality Mini LED Flood Light SMD Flood Light 150W: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[ Plastic Ceiling Rose]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Carton Steel Wedge Anchor High Quality Supplier]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Flip PU Tablet Case Cover for iPad iPad Mini 4 Cover]

Low Price Fashion Unique Plastic Zipper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Silica Gel- Indicating Blue to Pink After Adsorption-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Custom Clamshell Book Shape Black Cardboard Magnet Gift Box]

Low Price Fashion Unique Plastic Zipper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Optical Fiber Connector Cleaning Cassette Reel Cleaner]

Low Price Fashion Unique Plastic Zipper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Offer High Quality Square and Round Cast Iron Manhole Cover, Part I.White Tight Long Sleeves off Shoulders Celebration Bandage Dress. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane)]

OEM Precision Electronic Industry Machined/Machining CNC Brass Connectors, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4X3200 2mm 3mm 4mm Nc Steel Metal Aluminum QC12y Shearing Machine and Power Shearing Machine.Road Safety Reflective Waterproof Plastic Reflectors, 175-184[Yhcb Series Sliding Vane Pump for Tanker Truck]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[COB LED Integrated All in One Solar Street Light with LiFePO4 Lithium Battery]

Whitaker,J.R.(1963),Mirror 3003 H12 Aluminium Chequered Sheet for Baking Sheet in Hungary. Analytical Chemistry35(12), 1950-1953[2019 Hot Sale High Quality Chinese Factory Direct Supplied Heavy Duty 3-200ton Electric Winch]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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