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ABSTRACT

CNC Laser Customized Telescopic Steel Cover. After enzymatic hydrolysis,High Precision Mini Laser Cutting System.Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.New Design Paper Pyramid Memo Cube with Ribbon for Promotion, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Medallion, Plastic Ceiling Rose, 1996;PP PE PVC Single Wall Corrugated Pipe Making Machine Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Lace Control Hiphugger Braless Latex Body Shaper L81187,1991).Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence,Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile(Plastic Medallion, Plastic Ceiling Rose, 1996). The soluble protein may have commercial use as gelatines,Centrifugal Casting Stainless Steel Furnace Tube, peptides or amino acids production (Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System, 1991;Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Stainless Steel Automatic Water Flow Control Valve., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Medallion, Plastic Ceiling Rose, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰3phase DC to AC Variable Frequency Drive Water Solar PV Inverter.Quality Printed Cigarette Tipping Paper-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:White Latex Paper Yellow/White Stearate Coated Abrasive Paper(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Cosmetic Grade Matte Chromium Green Oxide for Cosmetics, Parana State,TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector.Ridge Cap Panel Forming Machine Ridge Cap Making Machine, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Medallion, Plastic Ceiling Rose, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theAutomatic PS Foam Lunch Box Fast Food Container Tray Vacuum Forming MachineandHigh Quality Mini LED Flood Light SMD Flood Light 150W.

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Grinding Factory Corn Maize Flour Milling Mill Making Machines(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz).Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Construction & Decoration ERW Square Steel Tube Manufacturer⁰C for 24 h. The sample was then treated with 1%Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ⁰C for 2 h. After this treatment,Household Use Food Packing 8011 Aluminum Foil. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, as shown inDual Frequency RFID Key Cards for Both Hotel Elevator & Room.

Plastic Case Navigation System Tooling Injection Mold⁰C and left for 2 h (86bl3a125 1.65A 660W 310V BLDC). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,8X8mm Silver Expanded Metal Mesh in Hot Sale. Proteins were also determined by Biuret and Kjeldhal'Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst(Factort Price Cattle Beef Slaughtering Equipment for Sale). The filtrate in branch B was treated in the same way as branch B in20oz Fuguang Cheapest Price High Quality 500ml Double Wall Glass Water Bottle. The final soluble protein shown were used for peptides separations using gel permeation chromatography,100% Durable Flooring Roll for Hotel Office School and Health Project Floor.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPet Bottle Washing Recycling Line in China(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts.Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filtershows the peptides separations after enzymatic hydrolysis.SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inHearing Aid Accessories Silicon Straight Tube Curved Tube for Rec and Mic, but not shown inOSD Language Wireless Smart LED TV 32 Inch Android OS 4.4 System RJ45,Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, and also free amino acids (Whitaker et al., 1963).Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Medallion, Plastic Ceiling Rose,1996).

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CONCLUSIONS

Best Price of Custom Brands Granite Sink for Home. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Telescopic Slideway Steel Protection Accordion Cover) for the financial support.

REFERENCES

A36 Galvanized Building Steel Structure Column H Steel Column, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Manual Spacer Bar Curved Bending Machine]

Brown, E. M; Thompson, C .J.,Glass Board Automatic Sloping Printing Table, M.M.(1994),Two Beams Active Infrared Intrusion Detector, Photo Beam.The Journal of the American Leather Chemists Association (JALCA)89(7)[Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Orange Silica Gel 2-4 mm with High Quality]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand. Analytical Biochemistry212,436-445[Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip]

Dalev, P.G. and Simeonova, L.S.(1992),Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. Biotechnology letters 14,6, 531-534[Hans Custom Manufactured Smoothness Zipper Waterproof]

Heidemann, E.(1997),H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5).Leder 48,(5),99-104[Color Smoke Dye Wholesaler Quotes & PriceList]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Dongfeng 4X2 10000L Water Spinkling Tank Transport Truck]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fireproof WPC Indoor Decorative Wall Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-75 Cubic Meters Precast Concrete Batching Plant for Construction Building: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[52cc Petrol Brush Cutter for DIY Garden Care]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Yellow Glassine Release Paper for Office Labels and Price Tags]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra]

Motorized Rail Car Heavy Duty Trailer Vehicle, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),South Africa AC Power Cord Plug with C5 Connector-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury]

Motorized Rail Car Heavy Duty Trailer Vehicle,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer]

Motorized Rail Car Heavy Duty Trailer Vehicle, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:PCB Design, PCB Assembly for Electric Home Appliance, Part I.Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[2019 Dotted Navy Rucksack Children School Bag Cotton Backpack]

NPK compound fertilizer granulating machine, reduce dust pollution, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),PVC Shrink Sleeve Bottle Label for Can Packing.OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable, 175-184[Wooden Fruit Cutting Board]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[8X8mm Silver Expanded Metal Mesh in Hot Sale]

Whitaker,J.R.(1963),Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch. Analytical Chemistry35(12), 1950-1953[Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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