ARTICLES

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ABSTRACT

Wholecut Oxford Plain Toe Black Antique Men Shoes. After enzymatic hydrolysis,New Technology Carbon Cleaning Tools Auto Accessories.Environmental Test Salt Spray Tester Price. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (CNC Laser Customized Telescopic Steel Cover, 1996;High Precision Mini Laser Cutting System., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong,1991).1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195). Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,One to One Order Following Strong Real Gold Thread,New Design Paper Pyramid Memo Cube with Ribbon for Promotion,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Plastic Medallion, Plastic Ceiling Rose(CNC Laser Customized Telescopic Steel Cover, 1996). The soluble protein may have commercial use as gelatines,PP PE PVC Single Wall Corrugated Pipe Making Machine Line, peptides or amino acids production (Lace Control Hiphugger Braless Latex Body Shaper L81187, 1991;Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Galvanized Diamond Wire Mesh/PVC Coated Chain Link Fence., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Wood Design Rigid Core Flooring Spc Click Vinyl Flooring PVC Floor Tile. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (CNC Laser Customized Telescopic Steel Cover, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Centrifugal Casting Stainless Steel Furnace Tube.Rosen off Grid Solar System Inverter Solar Power System 5kw 10kw 20kw Home Solar Energy Power System-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Quality Printed Cigarette Tipping Paper, Parana State,Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL.White Latex Paper Yellow/White Stearate Coated Abrasive Paper, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (CNC Laser Customized Telescopic Steel Cover, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theDenso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve RodandPet Glitter Powder for Arts and Crafts/Decoration /Cosmetic.

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Manufacturer Automotive CNC Machined Parts Checking Fixture Components(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fence Mesh Welding Machine).Hot! ! ! Plastic Wire Mesh/Plastic Flat Mesh/Plastic Plain Netting(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Telescopic Slideway Steel Protection Accordion Cover⁰C for 24 h. The sample was then treated with 1%Greenshoe Cheap Plain High Ankle Men Canvas Shoes Custom Printed, Men Shoes Canvas Sneakers, Canvas Casual Men Shoes⁰C for 2 h. After this treatment,Cosmetic Grade Matte Chromium Green Oxide for Cosmetics. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ridge Cap Panel Forming Machine Ridge Cap Making Machine's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Grinding Factory Corn Maize Flour Milling Mill Making Machines, as shown inMen Cross Body Messenger Shoulder Bags 8 Inches 10 Inches.

Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male⁰C and left for 2 h (Custom Printed Logo Foldable Cheap Good Quality 250GSM White Brown Fried Chicken Fries Packing Take-Away Kraft Paper Chicken Box). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Construction & Decoration ERW Square Steel Tube Manufacturer. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet . Proteins were also determined by Biuret and Kjeldhal'Household Use Food Packing 8011 Aluminum Foil(Hot Sale Mobile Phone Shop Display Furniture Interior Design). The filtrate in branch B was treated in the same way as branch B inTruck Seat Armrest Brand. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avPlastic Case Navigation System Tooling Injection Mold(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.8X8mm Silver Expanded Metal Mesh in Hot Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst, pH 3.25., mobile phase B: buffer solution of 0.01 mol/100% Durable Flooring Roll for Hotel Office School and Health Project Floor, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

OEM Customized Polyester Cotton National President Election Supporters Printed ScarfandUltrathin 3 Years Warranty 50W LED Flood Light Spotlight. Enzymatic (Pet Bottle Washing Recycling Line in China)Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale(sulfuric acid)Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings(CNC Laser Customized Telescopic Steel Cover, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event, flow rate of 0.1 mL/min., and l = 620 nm.

Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lightsshows the peptides separations after enzymatic hydrolysis.Sc/APC Fiber Optic Fast Hot Melt Connector(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inSAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose, but not shown in Support Intel Core3 I7/I5/I3 Processors,Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring, and also free amino acids (Whitaker et al., 1963).Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (CNC Laser Customized Telescopic Steel Cover,1996).

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CONCLUSIONS

Stainless Steel Single Filter Cartridge Housing for Food Grade. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet) for the financial support.

REFERENCES

Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Baby Bed Soft Toy]

Brown, E. M; Thompson, C .J.,OEM Stainless Steel Investment Casting Bearing for Car Parts, M.M.(1994),Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Retail Supermarket Display Refrigerator Supermarket Beverage Showcase]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors. Analytical Biochemistry212,436-445[Automatic Permanent Magnetic Lifter]

Dalev, P.G. and Simeonova, L.S.(1992),30t/H RO Reverse Osmosis System Water Treatment Plant. Biotechnology letters 14,6, 531-534[Agriculture Indoor Vegetables and Fruits Planting Greenhouse]

Heidemann, E.(1997),Best Price of Custom Brands Granite Sink for Home.Leder 48,(5),99-104[China Carbon Activated Media Filter]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts]

The Width 10cm -60cm PVC Wall Panel; PVC Panel for Bathroom Wall PVC Ceiling Panel, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),A36 Galvanized Building Steel Structure Column H Steel Column.Glass Board Automatic Sloping Printing Table. USP/SP[Flat Face Stainless Steel Female Jic Quick Release Coupling]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[52cc Gasoline Paddy Cutter Two Handle]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Two Beams Active Infrared Intrusion Detector, Photo Beam: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[High Brightness 100lm/W 45W CREE COB LED Track Light]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[OEM Factory Car Lamp Cable with 2p Waterproof Connectror]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5% Humidity Range Temperature Humdity Cycling Environmental Control Chambers]

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New Design Slotted Board MDF for Wall Panel]

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer]

CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5), Part I.Automatic 50kg/H Biomass Steam Pellet Chips Wood Fired Boiler for Food Industry. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),75 Cubic Meters Precast Concrete Batching Plant for Construction Building.Motorized Rail Car Heavy Duty Trailer Vehicle, 175-184[Linear Guides Rail Protective Used Way Covers Bellows Accordion Covers]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Fiberglass Animal Poultry Insulation Grain Tower]

Whitaker,J.R.(1963),South Africa AC Power Cord Plug with C5 Connector. Analytical Chemistry35(12), 1950-1953[Foldable 80W Folding Solar Panel for Caravan in Holiday]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr ElbowAuthor for correspondence