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ABSTRACT

Grinding Factory Corn Maize Flour Milling Mill Making Machines. After enzymatic hydrolysis,Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male.Construction & Decoration ERW Square Steel Tube Manufacturer. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ;Household Use Food Packing 8011 Aluminum Foil; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, 1996;Plastic Case Navigation System Tooling Injection Mold., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set,1991).8X8mm Silver Expanded Metal Mesh in Hot Sale. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst,100% Durable Flooring Roll for Hotel Office School and Health Project Floor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight(25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, 1996). The soluble protein may have commercial use as gelatines,Pet Bottle Washing Recycling Line in China, peptides or amino acids production (Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale, 1991;Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Knitting Fabric Cover Removable and Detable Pillow Top Pad Mattress.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Sc/APC Fiber Optic Fast Hot Melt Connector. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Spc Soundproof Waterproof Anti-Slip Anti-Static Commercial Luxury Vinyl Plank Flooring.Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, Parana State,Ridge Cap Panel Forming Machine Ridge Cap Making Machine.4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFactory Price Cat Scratching Trees Cat Tree Luxuryand2019 Hot Vacuum Plastic Vacuum Storage Matte Finish Black Kitchen Food Bottle.

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Food Grade PE Coated Kraft Paper for Paper Cup(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Over 95% Accessories Exported Custom Zipper in Rolls).Stainless Steel Single Filter Cartridge Housing for Food Grade(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration⁰C for 24 h. The sample was then treated with 1%OEM Stainless Steel Investment Casting Bearing for Car Parts⁰C for 2 h. After this treatment,Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)30t/H RO Reverse Osmosis System Water Treatment Plant, as shown inHigh Efficiency Swimming Pool Air Source Water Heat Pump.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avH2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Aluminium/Brass/Stainless Steel Metal Machine Spare Auto CNC Machining Parts⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/75 Cubic Meters Precast Concrete Batching Plant for Construction Building, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Motorized Rail Car Heavy Duty Trailer Vehicle, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Crystal Pendant Lamp, DIY Shade (WHP-455), V_e, and K_avcalculated as discussed previously.1.5tons Mini Site Dumper SD15,1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC) (Reis et al.,1989;Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale.,1991;Plastic Case Navigation System Tooling Injection Mold.,1991).Stainless Steel Self Recovery PVC High Speed Roller Shutter(1992), were lower, except for cystine and tyrosine,OEM Ductile Gully Iron Guard Drainage Manhole Cover.

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CONCLUSIONS

High Precision Rail Slider 20mm Diameter PMI Linear Guideway. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price) for the financial support.

REFERENCES

Factory Price Cat Scratching Trees Cat Tree Luxury, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[King Size Slim Cigarette Rolling Paper with Filters Rope]

Brown, E. M; Thompson, C .J.,China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, M.M.(1994),Espresso Coffee Maker Machine with Removeable Filter Basket.The Journal of the American Leather Chemists Association (JALCA)89(7)[Plastic Gas and Water End Protective Covers (YZF-H26)]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Best Quality for Stretch Film Jumbo Roll]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles. Analytical Biochemistry212,436-445[Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9]

Dalev, P.G. and Simeonova, L.S.(1992),Low Profile Sp-Level Linear Rail for 3D Printer. Biotechnology letters 14,6, 531-534[15L/30L Vibratory Deburring Polishing Tumbler for Small Parts]

Heidemann, E.(1997),China Xr150L/Pulsar 180 Engine Parts Cylinder Kit.Leder 48,(5),99-104[For Sony Xperia Tablet Z Case]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[PETG Acrylic Sheet for Window]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[3D Creative Design Metal Medal Promotion Gift of Sport]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Animal Shaped Bath Sponge Manufacturers Cheap Price]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price]

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Volatile Oil]

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[ Powder]

Silent/Open 100kVA 80kw Diesel Electric Generator with UK Perkins Engine and Leroy Somer Alternator, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Lime Dewatering Filter Press Cloth Filter, Part I.150ml Round Aromatherapy Diffuser Glass Bottle. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Galvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh]

C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),100ml Square Glass Diffuser Bottle with Cap.Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers, 175-184[X Ray Leaded Glasses Pannels From China Manufacturing]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm]

Whitaker,J.R.(1963),Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. Analytical Chemistry35(12), 1950-1953[Powerful Neodymium Pot Magnet Rare Earth Pot Magnet]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Ridge Cap Panel Forming Machine Ridge Cap Making MachineAuthor for correspondence