ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine

ABSTRACT

High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine. After enzymatic hydrolysis,CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal.Women Luxury Handbags Leather Shoulder Bag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Long Stroke Hydraulic Cylinder for Constraction Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Neoprene Protective Elbow Protector Brace Elbow Support (NS0012), 1996;Big Smart Plant Hydroponic Planter with Indoor Garden Light., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric,1991).High Quality Anti-Corrosion Fiberglass Sheet and Roll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Durable Steel Retainer Plastic Road Safety Trench Cover,Industrial Low Temperature Water Cooled Scroll Water Chiller,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)(Neoprene Protective Elbow Protector Brace Elbow Support (NS0012), 1996). The soluble protein may have commercial use as gelatines,EV Charger for 120KW DC Fast Electric Car Charging Station, peptides or amino acids production (Custom Velvet Jewelry Box Gift Box with Bowknots for Packing, 1991;Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aluminum Waterproof Folding Marquee Canopy Quick up Tent.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-4-20mA Current Sensor with Current Meter. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Neoprene Protective Elbow Protector Brace Elbow Support (NS0012), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Tc Made Boy′s Short Pants.Light Weight Ohsas 18001 Certified Metal Arch Buildings-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:New 60W LED Beam Zoom Moving Head with Prism(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Die-Casting Aluminium Radiator (RN808-500)(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts).Fr4 Double Sided PCB Fr4 PCB with Blue Solder Mask Custom Printed Circuit Board OEM with UL(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Wholecut Oxford Plain Toe Black Antique Men Shoes⁰C for 24 h. The sample was then treated with 1%New Technology Carbon Cleaning Tools Auto Accessories⁰C for 2 h. After this treatment,Environmental Test Salt Spray Tester Price. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Custom 2 Ring Binder PP Lever Arch Clip File Folder's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Quantum Science Lava Stone Pendant Necklaces Scalar Energy Pendant, as shown inTop Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator.

TUV Approved Y Splitter 2 Way Solar Mc4 Y Branch Connector⁰C and left for 2 h (Good Price Servo Motor Running OPP Film Bag Sealing Big Cushion Packing Machine). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.CNC Laser Customized Telescopic Steel Cover. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,High Precision Mini Laser Cutting System. Proteins were also determined by Biuret and Kjeldhal'Mill Finish Black Steel Grating Stock Panel Grating From Ningbo Jiulong(Factory Good Quality High Precision Machined Milling CNC Brass Parts). The filtrate in branch B was treated in the same way as branch B inToriconical Conical Head. The final soluble protein shown were used for peptides separations using gel permeation chromatography,1350ml Round Shape Borosilcate Lunch Box Office Used (GB13G17195).

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%One to One Order Following Strong Real Gold Thread. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew Design Paper Pyramid Memo Cube with Ribbon for Promotion(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.PP PE PVC Single Wall Corrugated Pipe Making Machine Line⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Lace Control Hiphugger Braless Latex Body Shaper L81187, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Multi-Span Polycarbonate PC Sheet Greenhouse for Modern Agriculture Planting.Fog Smoke Machine 1500W for LED Moving Head Beam Light DMXshows the peptides separations after enzymatic hydrolysis.Stainless Steel Automatic Water Flow Control Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMulti-Function WiFi Control Table Laser Cutting Engraving Machine Supplier, but not shown inBag Accessories 10mm Metal Dog Swivel Snap Hook,Mini Combine, Mini Harvester, Small Harvester Model 4L-1.0, and also free amino acids (Whitaker et al., 1963).Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Neoprene Protective Elbow Protector Brace Elbow Support (NS0012),1996).

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CONCLUSIONS

Ridge Cap Panel Forming Machine Ridge Cap Making Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Quality LED Wall Mirror with Ce, UL, cUL Certificate) for the financial support.

REFERENCES

Grinding Factory Corn Maize Flour Milling Mill Making Machines, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Wooden Lid Spice Jar]

Brown, E. M; Thompson, C .J.,Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male, M.M.(1994),Construction & Decoration ERW Square Steel Tube Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[Aluminium Glass Dropper for Glass Dropper Bottle]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Wholesale Professional Indoor&Outdoor Trampoline Park]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet . Analytical Biochemistry212,436-445[36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel]

Dalev, P.G. and Simeonova, L.S.(1992),Household Use Food Packing 8011 Aluminum Foil. Biotechnology letters 14,6, 531-534[OEM Colored Replacement Silicone Rubber O Ring for Tap and Faucet Fitting]

Heidemann, E.(1997),Solid PVC Forex Sheet 1.22*2.44m White Celuka PVC Foam Board.Leder 48,(5),99-104[High Purity Raw Powder Natamycin CAS: 7681-93-8 with Low Price]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Wireless Remote Control Sex Toys Pussy Men Anal Vibrator Prostate Massager for Male]

Stainless Steel Watch, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale.Plastic Case Navigation System Tooling Injection Mold. USP/SP[Custom PVC Plastic Card Printed School/Student/Office/Staff/PVC/ID/Name/Business Cards with Your Design]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[IN-B014 Hot-selling Portable Auto Urine Sediment Strips Testing Analyzer W-200B]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Hy1003 Mixer Shower Set, Luxury Rainfalls Shower Set: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Stainless Steel SUS304 Low Pressure Vacuum Tube Solar Water Heater]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Black Matt Electric Heated Towel Rail for Bathroom]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[High Temperature & IR Drying Electronic Oven / Furnace]

8X8mm Silver Expanded Metal Mesh in Hot Sale, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Piston Step Combination Seal/Seal PTFE +Polyester Guide Ring Gst-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[IP44 Waterproof 3pin 16A Electrical Male and Female Industrial Plug and Socket]

8X8mm Silver Expanded Metal Mesh in Hot Sale,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[RF Shielding MRI Door with Brass Frame for RF Cage]

8X8mm Silver Expanded Metal Mesh in Hot Sale, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:100% Durable Flooring Roll for Hotel Office School and Health Project Floor, Part I.Ultrathin 3 Years Warranty 50W LED Flood Light Spotlight. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ 42′ LG LED TV Backlight Strips/Bars/Spears]

Pet Bottle Washing Recycling Line in China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Powerful Water Cooling 1390 Mixed Metal Nonmetal Laser Cutting Machine for Sale.Ningbo Smart High Quality Nes Speed Controller Plastic Pneumatic Fittings, 175-184[Perfume Bottle Pictures]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality 98% Feed Grade Betaine Anhydrous]

Whitaker,J.R.(1963),Silver Square Circles Side Skirted Mirror Top Dining Coffee Table for Wedding Event. Analytical Chemistry35(12), 1950-1953[Fiberglass Covered Insulated Flat/Square Aluminum Magnet Wire]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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