ARTICLES

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ABSTRACT

New Virgin Teflon PTFE Teflon Gaskets/Back up Rings. After enzymatic hydrolysis,Capacitive Push Button Membrane Keyboards.Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Dtp18650-2s2p 14.8V 2200mAh 2600mAh Customizable Li-ion 18650 Battery Pack, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper, 1996;12V 96LED Outdoor Lighting Solar Torch Light for Christmas., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line,1991).SMF/VRLA Lead Acid Battery AGM Storage Battery for UPS System VRLA 12V200ah Export to UAE, India, Bangladesh, Afghanistan, Pakistan, Africa. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Efficiency Swimming Pool Air Source Water Heat Pump,CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC Service,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Eco-Friendly Vegetable Oil Cast Iron Multi Frying Pan(Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper, 1996). The soluble protein may have commercial use as gelatines,Luxury Chocolate Color Metal Tin Storage Can Candy Packaging Box, peptides or amino acids production (QJ-M Moving small manual brick making machine with low cost, 1991;Wholesale Professional High Quality Cheap Women Girls Camisole Sports Top Yoga Bra, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Ductile Grey Iron Casting Sand Casting., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.OEM Packaging Custom Luxury Vape Pen Cases for Electronic Cigarette.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-PVC Hdo Construction Formworks. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Screw Dosing Feeder Used for Chicken Feed Plant.5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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99% Purity Caustic Soda Flakes From Direct Manufacture on Sale(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Rectangular Design Ceramic Small Glass Basin Sink for Bathroom).Bwb-306 Custom Printing Outdoor Sports Wristband(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Irregular Shape Hologram Label Printing⁰C for 24 h. The sample was then treated with 1%High Speed Rope Weaving Machine⁰C for 2 h. After this treatment,Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, as shown inOxalic Acid for 99.6% Purity.

Funny Kids Soft Play Maze Big Slide Indoor Soft Playground⁰C and left for 2 h (Tall Thin Lowes Wood Storage Cabinet (GSP20-008)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Large Prototype CNC Transmission Assembly Tractor Machinery Part. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,China Durable 25kg 50kg Red PP Woven Bag Sack. Proteins were also determined by Biuret and Kjeldhal'SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock(Duct Evaporative Air Cooler for Warehouse Factory). The filtrate in branch B was treated in the same way as branch B inChina Supplier Aluminium Hostial Equipment. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Mini Recorder Sound Box for Musical Box & Plush Toys.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Fake Fish Luminescent Tropical Fish Fish Tank Decoration. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avIpx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Top Grade 93% High Purity 5L Flow Home Use Medical Portable Oxygen Concentrator Generator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove, pH 3.25., mobile phase B: buffer solution of 0.01 mol/CZ300 Automatic Switching Galvanized Plate C Z Purlin Roll Forming Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Ring Die Bio Organic Fertilizer Pellet Mill Machine.Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedalshows the peptides separations after enzymatic hydrolysis.Hot Dipped Galvanized Deer Fence From Factory(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet, but not shown inHigh Capacity Vibrating Screen Manufacturer,High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce, and also free amino acids (Whitaker et al., 1963).Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Manufacturer Custom DSLR SLR Camera Sling Backpack with Zipper,1996).

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CONCLUSIONS

Factort Price Cattle Beef Slaughtering Equipment for Sale. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mesh Stainless Steel Scourer Iron Sponge Used of Galvanised) for the financial support.

REFERENCES

Supreme 524 Mv Sea Kayak, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Free Sample Custom Made Square Shaped Waterproof Rubber Silicone Luggage Tags with ID Card]

Brown, E. M; Thompson, C .J.,API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, M.M.(1994),1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets.The Journal of the American Leather Chemists Association (JALCA)89(7)[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shop Front Indoor Durable Hanging Banner Advertising Signs]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-High Definition Outdoor Video Advertising LED Billboard. Analytical Biochemistry212,436-445[304 Stainless Steel Expansion Anchor Bolt]

Dalev, P.G. and Simeonova, L.S.(1992),Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm. Biotechnology letters 14,6, 531-534[2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery]

Heidemann, E.(1997),304 Stainless Steel Expansion Anchor Bolt.Leder 48,(5),99-104[Low Voltage Copper Conductor PVC Wire]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Kids Playground Rubber Floor Mat]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Second Hand/Used Hot Rolling Mill/Equipment/Machine/Production Line]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Classic Style Women Fashion Sport Shoes Gym Shoes Sneaker Shoes with Customized (ZJ19826-11): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Biaxial Plastic Grid Used for Pearl Farm]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cheese Puffed Snacks Making Machine]

Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch]

Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hemp Recycle Polyester Blenched Jersey Knit Fabric]

Basic Dyes CAS 548-62-9 Violet 3 Gentian Violet Crystal on Paper, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Non-Slip Anti-Slip Stainless Steel Anti-Slip Crampon Outdoor Walking Hiking Climbing Snow Ice Crampons, Part I.OEM Composite Machining Phenolic Titanium Parts Products Supplier. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Color Coated Prepainted PPGI PPGL Steel Sheet]

DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing.Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail, 175-184[m.hengyaomachinery.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag]

Whitaker,J.R.(1963),Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film. Analytical Chemistry35(12), 1950-1953[Mini Succulent Planters]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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