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ABSTRACT

35kw Low Temperature Intergrated Chemical Industrial Evaporative Cooled Water Chiller. After enzymatic hydrolysis,Wooden Handle 14 PCS Brush Holder Magnetic Makeup Brushes Set.Foshan Terracotta Facade Panel for Commercial Building Curtain Wall. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;New Stype Indoor and Outdoor Playground Plastic Slide with Swing for Paly Set;Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Uic Standard Railway Type Y25 Bogie for Different Freight Wagon, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box, 1996;Factory Truck/Bus Spray Paint Booth /Industry Paint Booth., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Enamelled Heavy Duty Cast Iron Skillet,1991).Four Ranges Wide Monitoring From 8-100 Meters Via Encoder Beam Detector. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Rd80 New Aluminum Alloy 3 Inch Industrial Chemical Resistant Water Diaphragm Pumps,Lab Equipment 3 Part Blood Cell Counter Auto Hematology Analyzer,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Ball Mill, Grinding Mill, Mining Equipment(Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box, 1996). The soluble protein may have commercial use as gelatines,Used Tyre Recycling Plant for Reclaimed Rubber Production, peptides or amino acids production (Fan Speed Controller RF Transmitter and Receiver Control Switch Kl-K201c, 1991;Robe Pointe 10r Sharpy 280W Beam Spot Wash Moving Head, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Medical Ce Approved Bp Machine Blood Pressure Monitor., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Max/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-2 Layers Large Plastic Blow Molding Machine/Blowing Moulding Machiery. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Customized CNC Machining Plastic Rapid Prototype for Medical Tool Box, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Customized CNC Precision Micro Machining Parts with Screw Thread Mirror Polish.Wireless CMOS Car Backup Camera with 7 Split Quad DVR LED Monitor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Jieshuai 3 in 1 USB Cable, Fast Charging Data Cable, Universal Micro USB Cable, Lightning Cable, Type C Cable, Suitable Apple iPhone and Android Charge(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Galvanized Steel Barbed Wire Rope 8mm(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting).CNC Aluminum Laser Milling Machinery(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%High Porosity Zirconia Zro2 Foam Filter for Foundry⁰C for 24 h. The sample was then treated with 1%Human Peptides Pentadecapeptide Bpc 157/Bpc-157 2mg 5mg for Anti-depression⁰C for 2 h. After this treatment,Disposable Nonwoven 3ply Medical Surgical Hospital Face Mask with Earloops. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844), as shown in42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Anodized Perforated 3D Facade Windows Screen for Commercial Building. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFull Cream Milk Powder Filling Machine (XFF-G)(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ce Approved Cheap Medical Disposable Electrical Infusion Pump) for the financial support.

REFERENCES

Canvas Shoulder Weekend Shopping Big Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[G-Hwp Hot Spiral Winding Krah Drain-Pipe for Municipal Project]

Brown, E. M; Thompson, C .J.,High Visuable Reflective Tape for Vehicle Road Safety, M.M.(1994),Made in China Aluminum Extrusion Window and Door Profile.The Journal of the American Leather Chemists Association (JALCA)89(7)[Good Quanlity of Ue319an20z Hydraulic Oil Filter Element]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[High Pressure Low Volume 2900rpm electric Water Pump]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc. Analytical Biochemistry212,436-445[Fire Retardant Reflective Aluminium Foil Woven for Insulation Material]

Dalev, P.G. and Simeonova, L.S.(1992),Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. Biotechnology letters 14,6, 531-534[Cosmetic & Personal Care Grade Xanthan Gum]

Heidemann, E.(1997),304/304L 2b Stainless Steel Sheet and Steel Plate.Leder 48,(5),99-104[SS304/SS316L Stainless Steel Tube Pipe Fitting 45 Degree 2 Inch Male Welding Elbow]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CNC Machinery Aluminum and ABS Mock up Prototypes]

Low Noise Ventilating Blower Fan for Agriculture, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier.Plastic Injection for OEM Product. USP/SP[Thick Wall Large Diameter Aluminium Tube Manufacture]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Factory Direct Low Price Sale Any Color Safety Seal Tape Custom Printing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Honkon Tattoo Removal Pigmentation Lesions Removal for Beauty Instrument]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Commercial Vegetable Drying Equipment]

Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Capacitive Push Button Membrane Keyboards]

Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5]

Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Experienced Construction Steel Structure Building Design, Part I.Heavy Duty 16000L High Pressure Sewer Cleaning Truck. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater]

Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam.Portable Ink Date Printing Machine for Plastic Bag, 175-184[Dunnage Lvl Manufacturers Cheap Price]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Galvanized Metal Welded PVC Coated Wire Mesh Fence]

Whitaker,J.R.(1963),High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0. Analytical Chemistry35(12), 1950-1953[Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CruzAuthor for correspondence