ARTICLES

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ABSTRACT

Original SKF Deep Groove Ball Bearings 6208 6208-2z SKF 6208-2z. After enzymatic hydrolysis,Top Quality Sanitary Tube Shell Tube Heat Exchanger.Different Thickness Mirror Color Coated Almn1cu Almg2.5 Aluminium Coil with PVC. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Xinan Delphi 9308-621c Diesel Injection Valve 9308-621c Fuel Injector Control Valve 28239294 Common Rail Valve 9308z621c;High Precision iPhone Cases Fiber Laser Marking Machine; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Water Cooling Radiator Release Temperature Screw Air Compressor, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Injection Mold Maker for Plastic Fighter Toy, 1996;PCBA SMT Equipment PCB Depaneling Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (HDPE Pipe for Water Supply (OD: 16mm-800mm),1991).Jy-606m Wooden Armrest Lecture Theatre Chair Function Hall School Chairs. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,2019 Male & Female Stainless Steel Quick Coupling,Pikachu PU Squishy Slow PU Foam Slow Rising Squish Kind Toys,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Digital Floating Baby Care Products Frog Bath Design Thermometer(Injection Mold Maker for Plastic Fighter Toy, 1996). The soluble protein may have commercial use as gelatines,Vertical Granule Grain/Peanuts/Seeds/Sugar/Rice Packing Automatic Filling Packaging Machine, peptides or amino acids production (Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board, 1991;FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Colorful Asphalt Shingle for Roof Water Proof., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Durable Inline Hockey Skate Puck.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Injection Mold Maker for Plastic Fighter Toy, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰18mm Thickness Best Quality Poplar Birch Commercial Plywood.European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Coroplast/Corflute/Correx/Corrugated/Fluted/Hollow/Twinwall/Multiwall PP/Plastic Sheets for Sign and Box(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Antique Stainless Steel Sheets Thailand 430 Stainless Steel Sheet, Parana State,Luzhong-700 70HP 2WD Two Wheel Drive Farm Tractor.20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Injection Mold Maker for Plastic Fighter Toy, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theIndustrial High  Temperature  Resistant Smooth Flat Wire Power CableandStainless Steel Self Recovery PVC High Speed Roller Shutter.

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304 321 310 310S Welded Decorative Stainless Steel Pipe(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Plastic Accessory for Hair Clipper).Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS)(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Manual Electric Winch Small Winch 220V Winch⁰C for 24 h. The sample was then treated with 1%250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price⁰C for 2 h. After this treatment,Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Plastic PVC Business Credit Card Fresnel Lens Magnifier Hw-805A's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Good Quality Low Price 18mm Natural Ash Veneer Fancy Plywood, as shown in6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor.

Metal Stamping Bending Sheet Metal Fabrication for CNC Machinery Part⁰C and left for 2 h (RF Coaxail BNC Male to BNC Male Plug Connector Adapter). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Aluminum Plate Fin Heat Exchanger/Oil Cooler Core. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,3X Desk Clip-on Magnifying Glass Lamp LED Light PCB Inspection. Proteins were also determined by Biuret and Kjeldhal'3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet( Galvanized Steel Grating. 403). The filtrate in branch B was treated in the same way as branch B inCustomized 1cr17 Telescopic Bellow Cover for Guideway. The final soluble protein shown were used for peptides separations using gel permeation chromatography,5-Axis CNC Machining Billet Aluminum Engine Rotary Housing.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%EPE Foam Sleeve Net Cushion Air Protective Packaging for Glass Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avCustom Clamshell Book Shape Black Cardboard Magnet Gift Box(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Kids Folding Play Mat Kids Play Room Floor Mat, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Roller Window Shutter for Residential Usage, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press MachineandPre-Seasoned Cast Iron 5-Piece Cookware Set. Enzymatic (Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts)High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel(sulfuric acid)Hydraulic Hose SAE 100 R1AT(Injection Mold Maker for Plastic Fighter Toy, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine, flow rate of 0.1 mL/min., and l = 620 nm.

L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Small Harvester Model 4L-1.0shows the peptides separations after enzymatic hydrolysis.Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Remy Virgin Brazilian Hair Bundle, but not shown inFood Sanitary Stainless Steel 2000L Fresh Milk Collection Tank,Ts-Micro Portable Defense Loudspeaker System, and also free amino acids (Whitaker et al., 1963).Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Injection Mold Maker for Plastic Fighter Toy,1996).

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Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage BuildingandOEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)show the gel chromatographic separation of soluble protein with Sephadex G-Medical Supply for Laparoscopic Instrument (MIM Straight Scissors).86bl3a125 1.65A 660W 310V BLDC, and another peak at 45 mL not shown inCustomized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine, for peptides lower than 3000 Da.Tamper Evident Branded Printed Packing Pet Adhesive Security Tape(Condell et al.,1993) .

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530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle, V_e, and K_avcalculated as discussed previously.Hotel Use and Steel Material Poultry House for 10000 Chickens,50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce) (Reis et al.,1989;Exterior Fireproof Wall Decoration Skirting Kitchen Cabinet HPL Board.,1991;PCBA SMT Equipment PCB Depaneling Machine.,1991).Kontron One-Piece 10-Lead EKG Cable with Resisitance(1992), were lower, except for cystine and tyrosine,Best Price of Peanut Digger / Groundnut Harvester in Australian Africa.

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CONCLUSIONS

China Manufacture Table Type CNC Horizontal Boring Milling Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh) for the financial support.

REFERENCES

White Electrical Fireplace Warmer, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Large Diameter Elbows Fitting Carbon 4 Inch Schedule 40 Galvanized Steel Pipe Made in China]

Brown, E. M; Thompson, C .J.,Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor, M.M.(1994),Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod.The Journal of the American Leather Chemists Association (JALCA)89(7)[China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[2019 New Design Flat Panel Print 3D Microfiber Cartoon Bedding Set]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone. Analytical Biochemistry212,436-445[Custom Size Rare Earth Permanent Speaker Ring Ferrite Magnet]

Dalev, P.G. and Simeonova, L.S.(1992),1 Inch Stainless Steel Flexible Exhaust Pipe. Biotechnology letters 14,6, 531-534[Automatic Hexagonal Wire Netting Machine Gabion Mesh Knitting]

Heidemann, E.(1997),Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403.Leder 48,(5),99-104[3v micro vibrating motor]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor]

Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator. USP/SP[New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Ce Certificate High Security Steel Shackle Padlock: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[PVC Car Seat Belt Shoulder Protector 80438]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[fastlinepcb]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[ Anti-Slip Hot Sale Floor Mat]

Drive Shaft for Jmc 8-94134458-PT, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),DSG Ball Screw Fixed Seat Nut Support-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[ Rubber Tire Road Roller for Sale]

Drive Shaft for Jmc 8-94134458-PT,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Custom Printed Aluminum Foil Side Gusset Coffee Plastic Bag]

Drive Shaft for Jmc 8-94134458-PT, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car, Part I.Apex Digital UV Printers for Aluminum Board Printing UV6090. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[12V/24V]

24 Molds Commercial Ice Cream Cone Maker, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine.Fashion Children Fur Pompom Beanie Scarf Knit Set, 175-184[Saddle Leather PU/PVC for Sofa]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[4PCS High Quality Adjustable Wrench Set (FST-41)]

Whitaker,J.R.(1963),Steel Jacket Steam Insulation Pipeline Production Best Price. Analytical Chemistry35(12), 1950-1953[6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

CNC Manufacturer High Precision Custom CNC Machining Parts/ CNC ServiceAuthor for correspondence