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ABSTRACT

Plastic Gas and Water End Protective Covers (YZF-H26). After enzymatic hydrolysis,400W Emergency Backup Battery Powerstation Portable Solar Generator.Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Automatic Electric Hydraulic Metal Bending Machine;6082 and 6061 Aluminum CNC Machining Parts for Aircraft; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.1054 FRP Pressure Vessel for Water Softener & Water Treatment, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, 1996;Non Woven Fabric for Shoe Lining Shoe Inner Good Quality., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Tungsten Carbide Diamond Tool Circular Blade with Saw Tips for Woodworking,1991).HDPE Large Diameter Hollowness Wall Winding Pipe Production Line. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Non-Woven Medical Outside Earloop Mask Making Machine,Professional Family Nonstick Cast Iron Frying Pan,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric(Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, 1996). The soluble protein may have commercial use as gelatines,Fashionable Clothing Garment Paper Swing Tags (ST-01), peptides or amino acids production (Horizontal Directional Drilling Machine SHD28, 1991;Electrial Galvanized Conduit Box/Gi Pipe Fitting, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Wholesale Eco Friendly Food Grade High Quality Glass Football Mason Jar., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High quality Brass Forged Gate Valve with Casting Iron Handle.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Aluminum Lighting Flashlights Zoomable Rechargeable LED Tactical Flashlight Torch (SYSG-180820). UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Type B Stainless Steel Threaded Quick Connector Coupling for Water Oil Gas System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰High Lumen 150W 200W LED High Bay Lighting High Bay LED Light Fixture.Cog Mono Round Display LCD Module-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:TV Stand Carving European LED Lights Heating Fireplace (320SS)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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High Pressure Low Volume 2900rpm electric Water Pump⁰C and left for 2 h (CNC Maching Parts for Autos). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design Fashion Jewelry 925 Sterling Silver Shell Pearl Clavicle Chain Necklace Bar Necklace. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Haivol High Voltage 8.7/15kv Cold Shrinkable 1 Cores Outdoor Terminal Kit (Applicable of cable crosssection 240). Proteins were also determined by Biuret and Kjeldhal'Boat Windlass Truck Auto Manual Lifting Hoist Tractortraction Hand Winch(Factory Truck/Bus Spray Paint Booth /Industry Paint Booth). The filtrate in branch B was treated in the same way as branch B inHigh Precision Mould / Plastic Mould for Plastic Products. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Multifunction Copper Busbar Processing for Aluminum Material.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Men Genuine Leather Sling Shoulder Satchel Messenger Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avElectric DC 24V Geared Motor for Auto Doors(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.RF Shielding MRI Door with Brass Frame for RF Cage⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Geotextile Beg Used for Beach Restoration Geobag, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Reusable Silicone Food Storage Bag, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Oxalic Acid for 99.6% Purity

10mm, 15mm, 20mm Customize Thickness Aluminum Honeycomb Sheets, V_e, and K_avcalculated as discussed previously.Auto Focus 280W 300W Metal Wood Laser Cutting Machine 1325,High Capacity Vibrating Screen Manufacturer. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tall Thin Lowes Wood Storage Cabinet (GSP20-008)) (Reis et al.,1989;Horizontal Directional Drilling Machine SHD28.,1991;Non Woven Fabric for Shoe Lining Shoe Inner Good Quality.,1991).Electrochemical Polishing Tungsten Evaporation Materials(1992), were lower, except for cystine and tyrosine,Kids Sneakers School Shoes for Girls with Design Wholesale High-Quality Custom Dropshipping Running Shoes.

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CONCLUSIONS

White UTP Cat5e UK Type Tooless RJ45 Keystone Jack. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Silica Gel- Indicating Blue to Pink After Adsorption) for the financial support.

REFERENCES

High Quality Hot Plate Non-Stick Catering Equipment Snack Machine Stainless Steel Electric Sandwich Panini Contact Press Grill, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Raw Hormone Powders]

Brown, E. M; Thompson, C .J.,CNC Machined Parts Aluminum/Brass/Steel/Stainless Steel Parts, M.M.(1994),Cheese Puffed Snacks Making Machine.The Journal of the American Leather Chemists Association (JALCA)89(7)[Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[3mm/0.21mm Aluminum Cladding Building Material Aluminum Composite Plastic Sheet]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration. Analytical Biochemistry212,436-445[High Capacity Vibrating Screen Manufacturer]

Dalev, P.G. and Simeonova, L.S.(1992),China High Profit Automatic Pet Extruder Machine. Biotechnology letters 14,6, 531-534[High Quality Small Tea Bag Packing Machine Price]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[High Quality RV Electric Seating Recliner]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Zw20 Outdoor Pole Mounted Sf6 Gas Insulated Load Break Switch: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Reinforced Brass Coated Steel Wire for Hose Wire]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[AISI316 Resin Traps (Strainer) for Ion Exchange]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[PE Pipe in SDR 11 Pressing Rate for Fire-Control]

Steel Structure Construction Building Metal House, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),1/8 1/4 3/8 1/2 Bsp Male Thread Pipe Fitting-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Mini Electric Chain Saw Cutting Machine Price]

Steel Structure Construction Building Metal House,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode]

Steel Structure Construction Building Metal House, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Wireless GSM 3G 4G SIM Card Solar Powered WiFi Surveillance Camera for Outdoor Indoor Security Full HD 1080P Bullet IP Camera, Part I.Stainless Steel Sanitary Thread Pneumatic Butterfly Valve. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Hot Sale Classic Golden Painted Rectangular Mirrored Dining Table]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Shop Front Indoor Durable Hanging Banner Advertising Signs]

Whitaker,J.R.(1963),Plastic Bowl Producing Machine for Drink Cup, Jelly Cup, Yogurt Cup with Letters Printing. Analytical Chemistry35(12), 1950-1953[304 Stainless Steel Expansion Anchor Bolt]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

2 Layers Large Plastic Blow Molding Machine/Blowing Moulding MachieryAuthor for correspondence