ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-PVC Hdo Construction Formworks. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Screw Dosing Feeder Used for Chicken Feed Plant

ABSTRACT

5.5 to 7.5kw AC Frequency Inverter for Motor AC Drive. After enzymatic hydrolysis,Factory Direct Disposable Delivery Surgical Drape Set/Pack with Eo Sterile.Automatic Waste Paper Stripper. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Top Quality Sarms Yk11 Yk-11 Yk 11 Powder with CAS 1370003-76-1, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, 1996;Korea Glittery Heat Transfer Film /Vinyl for Clothes/T-Shirt Printing Cdg-16 Light Multi., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (99% Purity Caustic Soda Flakes From Direct Manufacture on Sale,1991).Bwb-306 Custom Printing Outdoor Sports Wristband. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Irregular Shape Hologram Label Printing,High Speed Rope Weaving Machine,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Cordless Power Tools 12V High Quality Rechargeable Drill Cordless Drill Portable Electric Drill(U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, 1996). The soluble protein may have commercial use as gelatines,Nij III Silicon Carbide + PE Anti Spalling Coating Bullet Proof Vest Ballistic Plate, peptides or amino acids production (10 Speed Sex Perineum Massage Butterfly Remote Vagina Vibration Clitoris Stimulation Vibrating Panties Sex Toys, 1991;Funny Kids Soft Play Maze Big Slide Indoor Soft Playground, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Large Prototype CNC Transmission Assembly Tractor Machinery Part., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Durable 25kg 50kg Red PP Woven Bag Sack.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (U Truck for Partition Wall System75*50*0.5mm/Partition Drywall Steel Frame Stud, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Mini Recorder Sound Box for Musical Box & Plush Toys.Fake Fish Luminescent Tropical Fish Fish Tank Decoration-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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1850mm*80mm Stable Power Laser Tube(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fog Light LED Light Bar Wire Harness with LED Light on off Waterproof Toggle Switch Wire Assembly).White Color Premium Quality Disposable Plastic PVC Apron(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Galvanized Bar Grating⁰C for 24 h. The sample was then treated with 1%Ring Die Bio Organic Fertilizer Pellet Mill Machine⁰C for 2 h. After this treatment,Hot Dipped Galvanized Deer Fence From Factory. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility, as shown inLaser Marking 20W 30W Fiber Galvo Laser Metal Engraver Price.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%High Quality RV Electric Seating Recliner. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Factort Price Cattle Beef Slaughtering Equipment for Sale⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Supreme 524 Mv Sea Kayak, pH 3.25., mobile phase B: buffer solution of 0.01 mol/API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Outdoor Portable Charcoal BBQ Grill Barbecue Camping Stove) for the financial support.

REFERENCES

Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Carbonless Paper Roll]

Brown, E. M; Thompson, C .J.,FTTH Optical Network Terminal for Gpon ONU and CATV, M.M.(1994),OEM Customized Polyester Cotton National President Election Supporters Printed Scarf.The Journal of the American Leather Chemists Association (JALCA)89(7)[RF Coaxail BNC Male to BNC Male Plug Connector Adapter]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[ Galvanized Steel Grating. 403]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-D8tc High Performance 3-Electrode Motorcycle Spark Plug. Analytical Biochemistry212,436-445[110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine]

Dalev, P.G. and Simeonova, L.S.(1992),Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads. Biotechnology letters 14,6, 531-534[ Small Harvester Model 4L-1.0]

Heidemann, E.(1997),Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging.Leder 48,(5),99-104[Hy-Filling Plastic Bottle Perform Blowing Machine/Milk or Juice Bottle Making Machine (Hot Filling) /Plastic Bottle Blowing Machine with High Capacity]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[ Remy Virgin Brazilian Hair Bundle]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Lightweight Compact 70d Ripstop Nylon Parachute Hammock]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Nice Appearance Brass Metal Sheet Stamping: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Fountain Solenoid Valve]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[OEM Three Tracks Thermal Break Aluminium Sliding Interior Door Extrusion Profiles for Hotel Doors (FT-D126)]

Hot Sale Starter Motor Testing Equipment for Truck, Bus, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Home Machine 2019 New Commercial Treadmill Gym Equipment-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Customized HSS M100*3.0 Large Thread Cutting Tools Cutter Used for CNC Milling Machine]

Hot Sale Starter Motor Testing Equipment for Truck, Bus,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[High Quality Insulated Double Wall Bamboo Glass Water Bottle]

Hot Sale Starter Motor Testing Equipment for Truck, Bus, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, Part I.Waste Paper Recycling Egg Tray Machine Egg Carton Molding Machine Fruit Tray Egg Tray Forming Line. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce]

Clothing Hard Tag Security Tag for Supermarket/Retail Store, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),150 Ton Hydraulic Press Machine for Cooking Pot Making.Mirror Face Style Acrylic Round Wall Mounted Hanging Fish Bowl, 175-184[www.ldkchina.com]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS)]

Whitaker,J.R.(1963),Hot Sale Flat New Design Ladies Winter Warm Shoes. Analytical Chemistry35(12), 1950-1953[Cheap Stainless Steel Mirror Home Panoramic 6 Person Residential Passenger Lift Elevator]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

China Factory Plant Manufacturers Hot Feed Rubber Extruder MachineAuthor for correspondence