ARTICLES

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^IICNPq/RHAE/biotechnology-1850mm*80mm Stable Power Laser Tube. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,White Color Premium Quality Disposable Plastic PVC Apron

ABSTRACT

Galvanized Bar Grating. After enzymatic hydrolysis,Ring Die Bio Organic Fertilizer Pellet Mill Machine.Hot Dipped Galvanized Deer Fence From Factory. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;High-Quality Glass Prp Tubes Centrifuge Kit Blood Collection Tube with Ce;Tire Rubber Powder Recycling Equipment for Sale Truck Tires Rubber Shredding Facility; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Cheap Customized Aluminium Sand Casting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, 1996;Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Washing Machine Components Stainless Countersunk External Tooth Lock Washers From China,1991).New Arrival Wafer Type Pneumatic Fluorine Lined Butterfly Valve. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,High Quality RV Electric Seating Recliner,2mm Mirror Glass/Coated Glass for LED, LCD, Computer Screen etc,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,100% Hand-Painted Canvas Oil Painting Wall Art for Trees(Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, 1996). The soluble protein may have commercial use as gelatines,Factort Price Cattle Beef Slaughtering Equipment for Sale, peptides or amino acids production (Supreme 524 Mv Sea Kayak, 1991;API 594 Super Duplex Steel Single Disc Dual Disc Plate Wafer Check Valve, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;1-40mm Thickness and 1.22m, 1.56m Size PVC Celuka Board Manufacturer for Cabinets., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Definition Outdoor Video Advertising LED Billboard.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰304 Stainless Steel Expansion Anchor Bolt.New Small Plug-in Insect Killer Mosquito Killing Lamp-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Wholesale Customize PU Leather Hardcover Notebook with Window(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

OEM Composite Machining Phenolic Titanium Parts Products Supplier, Parana State,White Color Premium Quality Disposable Plastic PVC Apron.DC 12V/24V Coil Voltage 40A General Purpose Power Relay 5pin, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theBsp 60° Male Cone Seat Carbon Steel Fittingsand86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting.

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Z511 Custom Logo Clamshell Foam Insert Black Kraft Cardboard Paper Packing(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids).Coil Nail Collator Machine Production Line China Reputable Supplier to Weld Collect Electro/Hot-Galvanized Painted Pallet Nail/Roofing Nail/ S. S Nail(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film⁰C for 24 h. The sample was then treated with 1%Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars⁰C for 2 h. After this treatment,Xtsky Automotive Wheel Hub Bearing (DAC37720037). The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Big Mouth 1L Water Drinking Glass Cup Juice Glass Jar Bottle's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear, as shown inUnisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood.

FTTH Optical Network Terminal for Gpon ONU and CATV⁰C and left for 2 h (Best Quality White Color Electric Curtain Anti UV Self-Adhesive Pdlc Smart Film). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.OEM Customized Polyester Cotton National President Election Supporters Printed Scarf. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,D8tc High Performance 3-Electrode Motorcycle Spark Plug. Proteins were also determined by Biuret and Kjeldhal'Blank Mouse Pad, Mouse Pad Material, Printable Mouse Pad, Blank Sublimation Mouse Pads(FDA Standard BBQ Skewer with Colorful Wooden handle). The filtrate in branch B was treated in the same way as branch B in750ml Red Glass Wine Bottles. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Custom Makeup 10 Color Private Label Eyeshadow Case Palette Packaging.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Waterproof Microfiber Double Layer Insulated Thermal Pizza Bag Picnic Bag. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av19m Mobile LED Display Billboard for Advertising Screens(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Hot Sale Starter Motor Testing Equipment for Truck, Bus⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Home Machine 2019 New Commercial Treadmill Gym Equipment, pH 3.25., mobile phase B: buffer solution of 0.01 mol/New 50ml Glass Spray Bottle Green Refillable Bottles Vial with Glass Dropper for Essential Oil Perfume Cosmetic, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves.Building Material Quick Install Types of Scaffolding Couplersshows the peptides separations after enzymatic hydrolysis.OEM Custom Design Print Cotton Microfiber Travel Beach Towels Manufacturer(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inTs-Micro Portable Defense Loudspeaker System, but not shown in4-20mA Current Sensor with Current Meter,China Factory Plant Manufacturers Hot Feed Rubber Extruder Machine, and also free amino acids (Whitaker et al., 1963).2019 RF Skin Tightening IPL Skin Rejuvenation Shr Hair Removal. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Plastic Stand up Coffee Food Packaging Bag with Resealable Zipper,1996).

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Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price, V_e, and K_avcalculated as discussed previously.Over Band Round Type Electro Magnetic Separator for Tramp Iron,Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (3kw 4kw 5kw off Grid Solar Inverter) (Reis et al.,1989;Supreme 524 Mv Sea Kayak.,1991;Non-Woven Greenhouse Insulation Felt Making Machine Production Line/ Sell Greenhouse Insulation Quilt Production Line.,1991).Automatic China Beef Cattle Sheep Tripe Washer Cleaning Machine(1992), were lower, except for cystine and tyrosine,0.3-30mm Jc-EPE220 EPE Foam Sheet Making Machine Manufacturer.

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CONCLUSIONS

Bgf-800 Pet Dry Laminating Machine. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (33kv/415V 4000kVA Onan Dyn11 3 Phase Distribution Oil Type Transformer) for the financial support.

REFERENCES

Dimmable Industrial Indoor Commercial Indoor LED Highbay Lighting for Warehouse, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ceramic Christmas Tree Decorations]

Brown, E. M; Thompson, C .J.,Plastic Bottle Shape Zipper Bag for Candy Snacks Gift Packaging, M.M.(1994),Tec-Sieve Heavy-Duty Expanded Metal Catwalk Grating.The Journal of the American Leather Chemists Association (JALCA)89(7)[Custom Velvet Jewelry Box Gift Box with Bowknots for Packing]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-New Style PE PVC Carbon Spiral Duct Cable Pipe Extrusion Line / Extruder Machine. Analytical Biochemistry212,436-445[Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush]

Dalev, P.G. and Simeonova, L.S.(1992),Polyester Cocoon Bobbins Thread for Household Textiles. Biotechnology letters 14,6, 531-534[ 115V AC Single Phase Electric Motor Washing Machine Motor]

Heidemann, E.(1997),Large Capacity Lab Furniture Biological Safety Cabinet Fume Hood.Leder 48,(5),99-104[4PCS Rubber Wood Handle Cheese Knife and Plane Set]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Flame Retardant FRP/GRP Round Pipe]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-White Crystal Fashion Bridal Jewelry Accessories Wedding Tiara: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[50d Cationic Spandex Stretch Fabric for Garment]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Custom 2D Engraved Soft Enamel Fun Ride Metal Medal]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ]

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Hospital Medical Stainless Steel Top and Base Bedside Locker Jas-109-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Construction & Decoration ERW Square Steel Tube Manufacturer]

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Fiber Reinforced Plastic GRP FRP Fiberglass Gratings]

Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Stainless Steel Pipe Fitting 304 Cross of 3/4 Inch, Part I.1/2 Inch 4sh 4sp Steel Wire spiral High Pressure Hose. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock]

Whitaker,J.R.(1963),High Quality Rolamento Agulha Needle Roller Bearing HK1512 HK2020 HK2030 Bearing. Analytical Chemistry35(12), 1950-1953[20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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