ARTICLES

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^IICNPq/RHAE/biotechnology-South Africa AC Power Cord Plug with C5 Connector. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,PCB Design, PCB Assembly for Electric Home Appliance

ABSTRACT

Heavy Duty Wholesale Dubai Organic Cotton Canvas Fabric Drawstring Muslin Bag. After enzymatic hydrolysis,NPK compound fertilizer granulating machine, reduce dust pollution.PVC Shrink Sleeve Bottle Label for Can Packing. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable;Micropore PP Pleated Cartridge Filter 10 20 30 40 Inch; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Large Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (300mm Stroke Linear Module Sliding Bearing for CNC System, 1996;PCB /PCBA Design Gerber Files HDI Multilayer Aluminum PCB Prototype PCB., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Luxury Hotel Furniture Antique Design Marble Side Table,1991).High Dust Holding Capacity HEPA Filter with Separator. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Crystal Pendant Lamp, DIY Shade (WHP-455),1.5tons Mini Site Dumper SD15,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube(300mm Stroke Linear Module Sliding Bearing for CNC System, 1996). The soluble protein may have commercial use as gelatines,Stainless Steel Self Recovery PVC High Speed Roller Shutter, peptides or amino acids production (OEM Ductile Gully Iron Guard Drainage Manhole Cover, 1991;High Precision Rail Slider 20mm Diameter PMI Linear Guideway, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Factory Price Cat Scratching Trees Cat Tree Luxury., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Espresso Coffee Maker Machine with Removeable Filter Basket. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (300mm Stroke Linear Module Sliding Bearing for CNC System, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Roof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles.Low Profile Sp-Level Linear Rail for 3D Printer-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Xr150L/Pulsar 180 Engine Parts Cylinder Kit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Wholesale Custom Printed Blank Wholesale 100% Cotton Hoodies, Parana State,PCB Design, PCB Assembly for Electric Home Appliance.Lime Dewatering Filter Press Cloth Filter, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (300mm Stroke Linear Module Sliding Bearing for CNC System, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theWholesale Dry Fit Plain Sport Suit Top and Pants Man′s Club T Shirt Team ShirtandBison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator.

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150ml Round Aromatherapy Diffuser Glass Bottle(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Electric Battery Flameless LED Tea Light).C22 Stick Hole Bee Balm /Lemon Balm Tea Bag Packing Machine(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%100ml Square Glass Diffuser Bottle with Cap⁰C for 24 h. The sample was then treated with 1%Stainless Steel Finger Beer Bottle Opener Sommelier Wedding Wine Openers⁰C for 2 h. After this treatment,Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Pn10 Pn16 Class125 One Stem No-Pin Wafer Butterfly Valve's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)17r 330W Beam Spot Wash DJ Disco Stage Moving Head Light Ce RoHS, as shown inElectrochemical Polishing Tungsten Evaporation Materials.

Reusable CAT6 UTP RJ45 8P8C Modular Plug⁰C and left for 2 h (High Quality 20PCS Colorful Makeup Brush Set). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Good Quality OEM Stainless Steel Casting Rings. Proteins were also determined by Biuret and Kjeldhal'Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet(Pond Self Priming Booster suction  Water Pump with Pressure Sensor). The filtrate in branch B was treated in the same way as branch B inBestsub Medium Sublimation Printable Notebook (SNB02). The final soluble protein shown were used for peptides separations using gel permeation chromatography,Customizable Logo Plastic Safety Walking Reflectors Chinese Factory.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFlame Retardant FRP/GRP Round Pipe(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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K-Pop Bts Baseball Hat Sport Anime Cap.Toughened Railing Glassshows the peptides separations after enzymatic hydrolysis.Q32 Series Tumble Belt Shot Blasting Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inMax/Min Freezer Temperature Digital Thermometer Sensor Probe Tpm-900/Tpm-910, but not shown inAntique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair,Home Daily Tealight Glass Holder, Glass Candle Holder, and also free amino acids (Whitaker et al., 1963).Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (300mm Stroke Linear Module Sliding Bearing for CNC System,1996).

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CONCLUSIONS

Retail Supermarket Display Refrigerator Supermarket Beverage Showcase. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning) for the financial support.

REFERENCES

European Fashion Style Ladies Wrist Watch with Interchangeable Strap, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[www.szfanshitong.com]

Brown, E. M; Thompson, C .J.,Reusable SS304 316L Stainless Steel Powder 5 10 20 Micron Sintered Filter Cartridge for Indusrial Oil Filter, M.M.(1994),Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer.The Journal of the American Leather Chemists Association (JALCA)89(7)[ 1.56m Size PVC Celuka Board Manufacturer for Cabinets]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Full Automatic Combination Weighing Packing Machine Jy-420A. Analytical Biochemistry212,436-445[Glass Wool Sandwich Panel Metal Roof Price/Glass Wool Board]

Dalev, P.G. and Simeonova, L.S.(1992),Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate. Biotechnology letters 14,6, 531-534[Ipx7 Ipx8 Immersion Tank Waterproof Rain Spray Environmental Test Machine]

Heidemann, E.(1997),Tfl Blue Rubber Transmission Belts/Automotive Spare Part.Leder 48,(5),99-104[Customized Strong Permanent Rare Earth Neodymium Ring Magnet for Industry]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[CF8m Air Actuated Valve Dn15]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Integrated Hydraulic Steering Control Units (BZZ1F SCU)]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[10mm Gold Plated Stainless Steel Balls G100 Polish Ball]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[High Quality PU/TPU Tape for Garment]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Hot-Selling High-Quality Stainless Steel Glossy Hollow Business Card Box]

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Best Selling Hydraulic Small Truck with Crane 10 Ton,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[TP304L Stainless Pipe /Heat Exchanger/Condenser Tube;]

Best Selling Hydraulic Small Truck with Crane 10 Ton, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery, Part I.New Design Disposable Trocars for Laparoscopic Surgery. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Geotextile Beg Used for Beach Restoration Geobag]

Disposable Medical Combined Bacterial Viral Hme Filter, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale.Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System, 175-184[High Pressure Steam Boiler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch]

Whitaker,J.R.(1963),3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. Analytical Chemistry35(12), 1950-1953[Ss304 Sanitary Welded Butterfly Valve]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

500kg Capacity 14m Height Semi Electric Scissor LiftAuthor for correspondence