ARTICLES

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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Plastic Injection for OEM Product

ABSTRACT

Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. After enzymatic hydrolysis,Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material.Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Experienced Construction Steel Structure Building Design;Heavy Duty 16000L High Pressure Sewer Cleaning Truck; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Winter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, 1996;Portable Ink Date Printing Machine for Plastic Bag., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0,1991).High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Air 2 Bluetooth 5.0 Wireless Earbuds Noise Canceling Sports Earphonesfor iPhone/Android Apple Airpods,Best Items Gh/Genatropin with Naked Vial,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Custom Size Gel Nail Polish Set Boxes with Sponge(Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, 1996). The soluble protein may have commercial use as gelatines,High Precision Ce Radial Drill Machine Z3040, peptides or amino acids production (Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9, 1991;Dual Frequency RFID Key Cards for Both Hotel Elevator & Room, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Hot-Sale Products GRP Tower for New Energy Industry. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Fgg 2b 304 Metal Circular Push Pull Connector.Thick Wall Large Diameter Aluminium Tube Manufacture-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Portable Hydraulic Power Pack 12V Hydraulic Power Unit(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Hans Custom Manufactured Smoothness Zipper Waterproof, Parana State,Plastic Injection for OEM Product.Quartz Liquid Powder Feeding Torch, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCE Certificated Wheel Loader (HQ912) with Agricultural TireandMc4 Solar PV Connector TUV Approval.

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Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Fashion 925 Sterling Silver Pendants Necklace Jewelry).Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Outdoor P5mm Programmable Bus LED Display Sign⁰C for 24 h. The sample was then treated with 1%AGM Separator for AGM Battery⁰C for 2 h. After this treatment,Electric Battery Flameless LED Tea Light. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)CE Certificated Wheel Loader (HQ912) with Agricultural Tire, as shown inFgg 2b 304 Metal Circular Push Pull Connector.

Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays⁰C and left for 2 h (Engine Starter Motor for Honda Cr-V 2.4L L4 2002-06 (Lester17844)). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cosmetic & Personal Care Grade Xanthan Gum. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Professiona Ozone Generator for Swimming Pool. Proteins were also determined by Biuret and Kjeldhal'High Precision Plastic Mold Manufacturer in China(China Factory New-Product-Best-Quality-Customized-Metal-Pressing). The filtrate in branch B was treated in the same way as branch B inSemi Automatic Strapping Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Fiberglass Animal Poultry Insulation Grain Tower.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avNew M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/DIN 934 Fin Hex Nut Zp M10, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Japanese Taiho Brand 4D95 Orignal Crank Main Bearing Excavator Engine Piston M408h Std Size.Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etcshows the peptides separations after enzymatic hydrolysis.Large Span Steel Structrure for Warehouse From China(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inOEM Custom Forged Auto Parts, but not shown inDnv 271 En 12079 3.5 Cbm Offshore Mud Skip,New Design Steel Prefab Layer Cage Chicken House Poultry Farm, and also free amino acids (Whitaker et al., 1963).Garden Solar Stake Lights Outdoor Solar Pathway Lights Waterproof, 14.9X3.3 Solar Landscape Lights for Garden Path, Walkway, Lawn. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Non Woven Geotextile Geobag Sadbag Used for Reservoir Lake Dam,1996).

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Cast Iron Frame-Plate Chemical Filter Press, V_e, and K_avcalculated as discussed previously.Hydrafacial Water Cleaning Skin Peel Facial Beauty Machine,Wholesale Personalized Soild Color Sheer Silk Organza Ribbon with Satin Edged for Gift Packaging. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Cheap Topmac Brand Mobile Concrete Mixing Plant) (Reis et al.,1989;Carbon Fiber Soft TPU Shockproof Back Cover Case for Huawei Honor V10 /Honor V9.,1991;Portable Ink Date Printing Machine for Plastic Bag.,1991).20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack(1992), were lower, except for cystine and tyrosine,One Step HBV 5 in 1 Rapid Test.

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CONCLUSIONS

Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar Lamp. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Anti-Theft EAS Hard Tag Tag) for the financial support.

REFERENCES

Pig Farm Cast Iron Pig Leakage Dung Floor, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Basswood Birch Beech Grain Laser Cutting Plywood for Crafts Toys]

Brown, E. M; Thompson, C .J.,Clutch Brake Booster for Yutong Zk6127h Bus, M.M.(1994),20399560 Oil Seal 125X148X9.7 for Volvo.The Journal of the American Leather Chemists Association (JALCA)89(7)[Low Price Fashion Unique Plastic Zipper]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Zoo-Tunes Portable Wireless Mini Character Speakers]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Horizontal Milling and Boring CNC Lathe Machine. Analytical Biochemistry212,436-445[Best Price of Custom Brands Granite Sink for Home]

Dalev, P.G. and Simeonova, L.S.(1992),Kids Playground Rubber Floor Mat. Biotechnology letters 14,6, 531-534[Wholesale Fashion Women Clothes Fashion Yaga Pants]

Heidemann, E.(1997),100t 3200 mm Simple Operation Hydraulic Bending Machine Used in Luminum Alloy Doors and Windows Industry.Leder 48,(5),99-104[LC Multistage Pump Mechanical Seal (KLLC)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Quality Stacking Plastic Crystal Bar Stool Chiavari Napoloen Chair]

304/304L 2b Stainless Steel Sheet and Steel Plate, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),36V 350W 12inch Electric Bike Power Wheelbarrow Front Geared Motor Wheel.Sinotruk 12 Wheels 35, 000 Litres LPG Tanker Truck. USP/SP[HDPE Skew 45 Degree Drainage Pipe Fitting Sewage Tee]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Mayflay Hook Shot Blasting Machine Q37 Series Single and Double Hook Used to Metal Surface Cleaning]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-All in One Make up Brushes Kit 10PCS Professional Travel Makeup Brush: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Heavy Duty 16000L High Pressure Sewer Cleaning Truck]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Disability Fitness Course]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Sinotruk 12 Wheels 35]

Nickel Plating Cloisonne Gold Enamel Metal Medal (068), M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),SLS 3D Printing ABS Nylon Prototyping Service Machining 3D-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[E0303 300n Activator Chiropractic Impulse Adjusting Instrument]

Nickel Plating Cloisonne Gold Enamel Metal Medal (068),M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Skytone 4 Channel Digital 1u Light Weight Power Amplifier]

Nickel Plating Cloisonne Gold Enamel Metal Medal (068), M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:China Supplier Laboratory Mixer for Comstic, Part I.Water and Oil Repellent Singeing Cement Plant Use Nomex Dust Collector Filter Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[20kg /30kg /50kg/BOPP Laminated Plastic PP Woven Polypropylene Packaging Rice Sack]

Fiber Optic Equipment Sc/APC Quick Connector Sc Fast Connector Field Assembly Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Optical PCB Bar Connector for Jst Scn 2.5mm.Empty Aluminum Cans Sleek 355ml for Beverage Packing, 175-184[Light Paneling Aluminum Honeycomb Sheet and Wood Color Sandwich Panel]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005]

Whitaker,J.R.(1963),4mm New Oak Laminate Kitchen Sheet Vinyl Plank Luxury PVC Flooring Lowes. Analytical Chemistry35(12), 1950-1953[Regular Small Size One Bowl Kitchen Sink Russia Market (BS-336-201M)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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