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ABSTRACT

Ceramic Dinnerware Set Stoneware Coffee Mug 5.5inch Bowl Hand Painted Dessert Plates. After enzymatic hydrolysis,Glass Gold Leaf Mix Aluminium Modern Design Mosaic for Wall Decorate (GF302).Military Tactical Backpack, Molle Rucksack Bug out Bag, Medium. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Low Volume Production Aluminum CNC Machining/Machine/Machinery Car Parts;SMD 5050 RGB Flexible LED Strip (60LED/m) with Factory Bottom Price; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Auto Spare Parts Car Ignition Coil 032905106b 032905106 0221603006, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, 1996;4.3 Inch Touch LCD Display Module Naked Industrial Tablet PC Computer., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Food Grade PE Coated Kraft Paper for Paper Cup,1991).Stainless Steel Single Filter Cartridge Housing for Food Grade. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Modern Wall Art Decor Interior 3D Effect Wall Panels for Home Decoration,OEM Stainless Steel Investment Casting Bearing for Car Parts,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Industrial Bridge Crane Remote Hoist Crane Wireless Remote Radio Remote Control System(Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, 1996). The soluble protein may have commercial use as gelatines,High Speed Straight Line Copper and Steel Cable or Wire Drawing Machine with Individual Motors, peptides or amino acids production (30t/H RO Reverse Osmosis System Water Treatment Plant, 1991;Best Price of Custom Brands Granite Sink for Home, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;A36 Galvanized Building Steel Structure Column H Steel Column., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Glass Board Automatic Sloping Printing Table.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Two Beams Active Infrared Intrusion Detector, Photo Beam. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Wholesale Customized Packing PP Onion Potatoes Garlic Packing Bags, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰CCM Continuous Caster/Curved Spray Continuous Casting Machine in Great Demand.Cashmere Loose Neck Pullover with Fancy Yarn-Cashmere Sweater-Sweater-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:H2309 H2310 H2311 H2312 H2313 H2314 H2315 H2316 (H2317 H2318 H2319 KMTA5)(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%1100 Extrusion Hot Cold Rolling Forging Aluminum Alloy Tube. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel Self Recovery PVC High Speed Roller Shutter(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.High Precision Rail Slider 20mm Diameter PMI Linear Guideway⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Factory Price Cat Scratching Trees Cat Tree Luxury, pH 3.25., mobile phase B: buffer solution of 0.01 mol/China Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

Reusable CAT6 UTP RJ45 8P8C Modular Plug. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (75 Cubic Meters Precast Concrete Batching Plant for Construction Building) for the financial support.

REFERENCES

3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Comb Bulk]

Brown, E. M; Thompson, C .J.,Good Quality OEM Stainless Steel Casting Rings, M.M.(1994),Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Customizable Logo Plastic Safety Walking Reflectors Chinese Factory. Analytical Biochemistry212,436-445[50d Cationic Spandex Stretch Fabric for Garment]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Construction & Decoration ERW Square Steel Tube Manufacturer]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[SMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Flexible Machine Guard Shield for Trailer Bellow Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant]

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom small plastic printed box for keychain (PET box)-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting]

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Horizontal Milling and Boring CNC Lathe Machine]

Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fence Mesh Welding Machine, Part I.20 Years Factory! Double Channel Surgical Syringe Pump. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Cog Mono Round Display LCD Module]

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Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Waterproof Outdoor Army Military Camouflage Printing Ripstop Polyester Fabric]

Whitaker,J.R.(1963),Q32 Series Tumble Belt Shot Blasting Machine. Analytical Chemistry35(12), 1950-1953[Tfl Blue Rubber Transmission Belts/Automotive Spare Part]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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