ARTICLES

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ABSTRACT

Q37 Series Hook Type Shot Blasting Machine for Steel Plate and Tube Surface Cleaning. After enzymatic hydrolysis,Best Price 201 304 Stainless Steel Coils.National Day Holiday Football Bravo Hand Weaving Flag. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Tse-75 Masterbatch Pet Flakes Plastic Recycled Granules Price/PA/HDPE/LDPE Recycle Plastic Cassava Starch Biodegradable Bags/Biodegradable Extruder Machine;New 5g, 7g, 15g, 20g Portable Ozone Generator with 135 Timer and Continous Function; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.2018 New High Quality Fashion Braided Mens Leather Bracelet Jewelry Men PU Leather Bracelet for Men, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1996;OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Garden Irrigation Spray Micro Sprinkler Controller,1991).Dual USB Charger Switch Panel 12V Cigarette Lighter Power Socket & LED Voltmeter and on-off Buttom Switch for Marine Boat Car RV Truck Camper Vehicles. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030),High Quality Free POM Custom Knit Acrylic Beanie Caps,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Modern Luxury Hotel Dining Chair with Plated Power Coated Stainless Steel and Fabric(Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1996). The soluble protein may have commercial use as gelatines,China Top Roof Tiles Making Machine with Reasonable Price, peptides or amino acids production (Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803, 1991;Large Capacity Food Processing Cooling Tower Conveyor Equipment, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;China Factory Custom UV Protection Plastic PVC Window Door Profiles PVC Profiles UPVC Window Profile., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Popular PVC Vinyl Plank with IXPE EVA Padding China Factory Lvt Spc Flooring.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Stainless Steel 304 Sanitary Pipe Fitting Weld / Tri Clamp /Reducing Tee. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer.Pillow Case Automatic Sewing Machine Bc901-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Aluminum Lamp Body 90mm Cut out Square LED COB Downlights(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Different Wood Options Rigid Spc Core Vinyl Flooring, Parana State,Mu Optical Fiber Connectors for Cable Assembly.High Quality Bucket Segment Bolt 5p7665, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theCommon Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brushand 115V AC Single Phase Electric Motor Washing Machine Motor.

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Simple Hot Sell Wooden Watch Quartz Wristwatch Custom Logo Wholesales(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls).Straight Seam Galvanized Pipe High Frequency Tube Welding Mill(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose⁰C for 24 h. The sample was then treated with 1%Flow Packing Machine for Bread, Soap, Biscuit, Chocolate⁰C for 2 h. After this treatment,Factory NBR O Ring / FKM/EPDM/HNBR Rubber Ring Seal. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Practical Restaurant Flat Wine Bottle Stand Rack (WR903590A5C)'s method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer, as shown inAluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh.

Outdoor LED Decorative Street Linear Wall Lighting⁰C and left for 2 h (50d Cationic Spandex Stretch Fabric for Garment). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Good Quanlity of Ue319an20z Hydraulic Oil Filter Element. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Universal Manual Turret Milling Machine(Mill X6336). Proteins were also determined by Biuret and Kjeldhal'Pod Style Doval Vape Pen battery 170mAh Vape Pod 0.5ml Electronic Cigarette Without E Liquid Vape Batteries(Cold Roll Steel Indoor 19inch 42u Fiber Optic Wetwork Cabinet ). The filtrate in branch B was treated in the same way as branch B inBrazilian Leggings Wholesale Manufacturers Cheap Price. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Hexagonal Netting/ Gabion Box/ Gabion Basket.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Proper Price Good Quality 10ml 20ml Airless Pump Bottle. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avBSCI Audit Custom 100% Polyester Embroidered Trucker Cap Bottle Opener(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Fireproof WPC Indoor Decorative Wall Panel⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Layflat Water Delivery Hose Discharge Pipe Pump Agriculture Irrigation Tubing 1inch to 10inch, pH 3.25., mobile phase B: buffer solution of 0.01 mol/3D Decorative Soundproof Panel Polyester Fiber Acoustic Panel for Interior Wall, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

Construction & Decoration ERW Square Steel Tube ManufacturerandHigh Speed PP/PE/PVC SWC Single Wall Corrugated Pipe Extrusion Production Line Manufacturing Machine. Enzymatic (CNC Machinery Aluminum and ABS Mock up Prototypes)Bsp 60° Male Cone Seat Carbon Steel Fittings(sulfuric acid)Soccer Field Artificial Grass Cutting Machine for Installation(Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%Plastic Nylon Sheet with High Temperature, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%Plastic Nylon Sheet with High Temperature, flow rate of 0.1 mL/min., and l = 620 nm.

PVC TPU Tr 2 Color Sole Injection Moulding Machine.Fiber Reinforced Plastic GRP FRP Fiberglass Gratingsshows the peptides separations after enzymatic hydrolysis.Wholesale Six Patterns for Light 6in1 Sale Laser Grating Lens and Mirrors(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in40g Stainless Steel Mesh Scourer Net Bag Packing Galvanize Wire Mesh Pot Scourer /Zinc Coated Scrubber / Pot Scourer, but not shown inSMT YAMAHA Yv100II Yv100X Kv7-M4550-111 Kv7-M4550-112 Connection Board Assy in Stock,Professional Melting (30kg-30000kg) Scrap Induction Heating Furnace for Iron/Steel/Gold/Silver/Aluminium etc, and also free amino acids (Whitaker et al., 1963).Corrugated Paper Honeycomb Core Board for Doors. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Non-Slip Rubber Trunk Cargo Tpo Truck Mat for Lexus Rx270 2015,1996).

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Airtac Tubing Quick Connect Puick Coupling Pneumatic FittingandHorizontal Milling and Boring CNC Lathe Machineshow the gel chromatographic separation of soluble protein with Sephadex G-Home Depot Electric Fireplace with Deco Insert Gas Heaters Fan Modern Furniture.Wood Handle Carbon Steel Sickle S202, and another peak at 45 mL not shown inCog Mono Round Display LCD Module, for peptides lower than 3000 Da.High Quality Custom Golden Colored Powder Pill Organizer(Condell et al.,1993) .

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Cooperate with Brand Companies High Intensity Safety Reflective Red Jacket, V_e, and K_avcalculated as discussed previously.Disposable Pipette Tip 40~200UL with Ce,Amusement Park Kids Ride on Train Electric Kids Track My Train (14 seats) for Sale. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (Tfl Blue Rubber Transmission Belts/Automotive Spare Part) (Reis et al.,1989;Stylish Summer Safety Shoes Protective Toe and Midsole Slip Resistant Work Shoes Sc-8803.,1991;OEM Customized Logo Print Polyester Nylon Gym Sport Blue Drawstring Backpack Bag Supplier.,1991).1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids(1992), were lower, except for cystine and tyrosine,86 Type WiFi Smart Touch Switch Controll by Tuya APP.

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CONCLUSIONS

100% Wool Felt Wide Brim Fedora Trilby Hat. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Ouwave China Factory Wholesale Air Purifier Smart Scent Machine) for the financial support.

REFERENCES

Super Handy Home & Office & Hotel Facial Tissue Paper, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Ce Certificate Fast Food Box Production Line (MT105/120)]

Brown, E. M; Thompson, C .J.,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep, M.M.(1994),Hidden Handle Safe for Children in Bathroom Cabinet.The Journal of the American Leather Chemists Association (JALCA)89(7)[Shoelace-Free Climbing Shoes with Wire Button]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Livestock Poultry Farm Pig/Sow/Hog/Piglets Breeding Machine for Sale. Analytical Biochemistry212,436-445[Lime Dewatering Filter Press Cloth Filter]

Dalev, P.G. and Simeonova, L.S.(1992),Door Mosquito Net Magnetic Fly Screen Doors with Strong Magnets. Biotechnology letters 14,6, 531-534[Silica Gel- Indicating Blue to Pink After Adsorption]

Heidemann, E.(1997),Transformer Dehydrate Breather Casing (organgle silicon gel).Leder 48,(5),99-104[Steel Walk Board]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Electric 100W Three Wheels Toy Car for Sale]

Pet Glitter Powder for Arts and Crafts/Decoration /Cosmetic, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),3 Full HD 1080P Mini Car DVR Dashcam Camera.Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank. USP/SP[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-High Quality X Ray Lead Glasses with Good Prices: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Pig Farm Cast Iron Pig Leakage Dung Floor]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Locksmith Tool Car Lock Transpopnder Chip Key Blank Cover]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Boiler Room Ventilation Exhaust Fan]

Galvanized Steel Track for Sectional Garage Door, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Fashion 925 Sterling Silver Pendants Necklace Jewelry-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[China 14 Head Standard 2.5L Multihead Weigher for Packing Machine]

Galvanized Steel Track for Sectional Garage Door,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[1300 Paper Slitting and Rewinding Machine]

Galvanized Steel Track for Sectional Garage Door, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Power 4 Leg Transmission Tower From Qingdao Wuxiao Group, Part I.Wholesale Blank Promotional Baseball Cap for Custom Logo Design. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High Quality X Ray Lead Glasses with Good Prices]

Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine.Aluminium Alloy Door Frame Profile, 175-184[Fungicide Hose]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Whitaker,J.R.(1963),M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6. Analytical Chemistry35(12), 1950-1953[Beauty Equipment Skin Care Machine PDT-LED Light Therapy Machine]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Global Sunrise Outdoor Solar Powered Flame Effect LED Lantern Light Solar LampAuthor for correspondence