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^IMestres em Química (Mestrado em Quimica Aplicada-UEM)
^IICNPq/RHAE/biotechnology-Bookcase, Cupboard, Filing Cabinet, Bookshelf, Melamine Cabinet. (*) Departamento de Química, Universidade Estadual de Maringa Av. Colombo, 5790.87020-900 Maringa, Parana,Customizable Logo Plastic Safety Walking Reflectors Chinese Factory

ABSTRACT

Best Quality Brass Bathroom Door Knob Kitchen Cupboard Door Knobs Handles Cabinet Handle. After enzymatic hydrolysis,Flame Retardant FRP/GRP Round Pipe.Psa Oxygen Generator for Industry (ISO9001, CE). Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;LED Pixel Addressable Madrix Silicon 360 Degree Flexible Neon Rope LED Strip Light Christmas Decoratios Wedding Decoration Lighting;P1.25 Full Color Cable Less Indoor Fixed LED Display, Fine Pitch LED Display; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Airtac Tubing Quick Connect Puick Coupling Pneumatic Fitting, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Custom small plastic printed box for keychain (PET box), 1996;Fence Mesh Welding Machine., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (20 Years Factory! Double Channel Surgical Syringe Pump,1991).Best Quality for Stretch Film Jumbo Roll. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Drum Type Magnetic Separator,K-Pop Bts Baseball Hat Sport Anime Cap,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Q32 Series Tumble Belt Shot Blasting Machine(Custom small plastic printed box for keychain (PET box), 1996). The soluble protein may have commercial use as gelatines,Home Daily Tealight Glass Holder, Glass Candle Holder, peptides or amino acids production (Baking Rotating Rack Diesel Gas Steam Oven Machine for Food, Cake, Buscuit, Croissant, 1991;Round Manufactory Non-Stick Silicone Stainless Dog Feeder, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Plastic Anchors Lag Expansion Nails Plugs Screws 100 PCS., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.15mm Thickness Aluminum Honeycomb Panel Forarchitectural Facades Panels Canopy Ceiling Signage Ventilated Facades.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Custom small plastic printed box for keychain (PET box), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Extendable 485mm Stun Guns Baton with Electric Shock for Self Defense (X10).Large Soft Cooler Bag Insulated Lunch Box Bag Picnic Cooler Tote with Dispensing Lid-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:High Lumen Outdoor IP65 100W LED Flood Light(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Production Line for Making Nonwoven Stiff Waddings and Glue-Free Waddings / Nonwoven Spray Glue Cotton Production Line/ Hot Melt Glue Sheet for Fabrics Laminate(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price).Tfl Blue Rubber Transmission Belts/Automotive Spare Part(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%1220*2440*15mm Chipboard with Plain Face for Furniture Decoration From China⁰C for 24 h. The sample was then treated with 1%Flat Wedge Wire Panel China Factory⁰C for 2 h. After this treatment,Large Size Auto Feeding Paper Cutter Composite CNC Digital Oscillation Circular Vibration Cutting Machine 1610 1625. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Best Selling Hydraulic Small Truck with Crane 10 Ton's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector, as shown inStraight Seam Galvanized Pipe High Frequency Tube Welding Mill.

CNC Pipe Bending Machine for Trolley Frame Wheelbarrow Wheel Barrow Making Machinery⁰C and left for 2 h (Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.New Design Disposable Trocars for Laparoscopic Surgery. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Disposable Medical Combined Bacterial Viral Hme Filter. Proteins were also determined by Biuret and Kjeldhal'Mini Cocoa Peanut Paste Grinding Butter Colloid Mill for Sale(Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier). The filtrate in branch B was treated in the same way as branch B inBaby Album Photos. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Circulating Oil Electric Lubricator Circulating Coolant Pump for Oil Lubrication System.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%3W 5W 7W 9W LED Bulb E27 E14 B22 LED Bulb. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avHigh Efficiency Pineapple Canning Plant(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Cheapest 250ml 300ml 400ml 500ml 800ml Voss Glass Water Bottles⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Casting PE Film with Ash for Adult Diaper Back Sheet, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Supra Brand Laundry Liquid Baby Formula, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Class 1/Class 2 Birch Plywood for Automotive and Boatbuilding Applications.5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nutshows the peptides separations after enzymatic hydrolysis.Cookies Cake Automatic Rotary Packing Machine(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inToyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector, but not shown inPre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design,Building Material Quick Install Types of Scaffolding Couplers, and also free amino acids (Whitaker et al., 1963).Latest New Upgraded High Security and Regular Keys Cutting Machine Sec-E9 for Automobile Industury. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Custom small plastic printed box for keychain (PET box),1996).

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Turning

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CONCLUSIONS

Color Stretch Film for Pallet Wrap Use. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (European Fashion Style Ladies Wrist Watch with Interchangeable Strap) for the financial support.

REFERENCES

Wholesale Virgin Mink Brazilian Bundle Hair Vendor, Remy Virgin Brazilian Hair Bundle, Virgin Raw Brazilian Cuticle Aligned Hair, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Metal Sintering Filters]

Brown, E. M; Thompson, C .J.,Hemp Recycle Polyester Blenched Jersey Knit Fabric, M.M.(1994),Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV.The Journal of the American Leather Chemists Association (JALCA)89(7)[Fr4 Copper Clad Laminate PCB Manufacture Company]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Clear Plastic Roofing Sheet Roof Tile Price Plastic Shingle Roof]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Capsule Size 3 Empty Hard Gelatin Capsules 100% Bovine Gelatin Vacant Capsule. Analytical Biochemistry212,436-445[Die Cutting Customized Polyester Hook Loop Adhesive Cable Protection Tape]

Dalev, P.G. and Simeonova, L.S.(1992),3D Creative Design Metal Medal Promotion Gift of Sport. Biotechnology letters 14,6, 531-534[Stainless Steel LED Underground Light]

Heidemann, E.(1997),HDPE/PP Krah Spiral Winding Sewage Pipe Extruding Production Line.Leder 48,(5),99-104[Special Square Nut According to Your Drawing]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Us FDA Authorised 1g Desiccant Silica Gel Used for Food/Medicine]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Brake Fluid Tank Container Hot Plate Plastic Welding Machine: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Standard Disposable Oxygen Mask/Nebulizer Mask with Tubing]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Easy-Installation 4.8W Solar Powered LED Spike Light for Garden/Path/Park Decoration]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Auto Connector Tyco AMP 1.5mm Series Male Female Connector Electrcial Wire Harness 1pin 2pin 3pin 4pin 5pin 6pin]

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Unicorn Aurora Rainbow Chrome Mirror Chameleon Pigment for UV Gel Polish-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[High Performance Fresh Air Handling Unit for Marine]

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[25X75m Big Temporary Storage Tent for Storage/ Warehouse Solution on Sale]

6 Shooter Multi-Band Ligator Used to Endoscopically Ligate Esophageal Varices, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Distributor Living Room Furniture Restaurant Chair Tiffany Chair for Wedding, Part I.High Quality Custom Stainless Steel/Brass/Bronze/ Precision  Auto Spare Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[ Pneumatic Punching Machine]

Puffed Core Filling Sanck Production Machine with Ce From China, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),86 Inch All-in-One Android System Interactive Touch LCD Kiosk for Education/Meeting.Cummins Fuel Injector Kit (3076736 3095023), 175-184[Ss304 Stainless Steel Seamless Pipe]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Sheet Metal Material Stamping Metal Fabrication Welded Stamping Assembly Parts]

Whitaker,J.R.(1963),Hnx IP68 Waterproof Nylon Plastic Cable Gland Pg13.5. Analytical Chemistry35(12), 1950-1953[High Quality Gas Spring Strut Gas Shock for Wall Bed]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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