ARTICLES

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ABSTRACT

High Quality Aluminum Street Light Pole. After enzymatic hydrolysis,WPC Wall Cladding Waterproof Exterior Wall Panels.Customized Permanent Strong Nickel Block Neodymium Magnet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Stainless Steel LED Underground Light, Ground Lighting IP66, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets), 1996;K Series Helical Bevel Reduction Gearbox Motor., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (OEM Service Ce/ISO Approved Endoscopic Disposable Grasping Forceps,1991).10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Mini Outdoor Hydraulic Man Aluminum Lift,Wholesale Fashion Women Clothes Fashion Yaga Pants,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,High Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride(GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets), 1996). The soluble protein may have commercial use as gelatines,Economic Full Automatic LED Pick and Place Machine for LED Strip Making, peptides or amino acids production (1/4 25FT Twin Line Welding Hose for Oxy-Acetylene, 1991;PETG Acrylic Sheet for Window, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;High Frequency Online UPS W/3W-20K., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Luxurious Fire and Burglary Proof Hidden Hotel Security Safe Box.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Fst800-211 Industrial Water Pressure Sensor and Transmitter with 4-20mA Output. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (GMP Certified Health Food Vitamin C Electrolyte Effervescent Tablets (4G/ tablet * 10 tablets), 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰14 Head Multihead Weigher for Pork with Screw Feeding.Stainless Steel Sanitary SS304/SS316 2 Way Tri Clamp Ball Valve-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:PSF 1000 Flat Plate Upper Discharge separator(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Indoor Tub Customized Shower/Free Standing Shower Enclosure(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Flame Retardant FRP/GRP Round Pipe).Glow in Dark Ceramic Sublimation Mug 11oz(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Double Poles Number Bimetal Strip 1AMP Mini Circuit Breaker⁰C for 24 h. The sample was then treated with 1%Stainless Steel 201/304/316 CF8/CF8m 1000wog Threaded Ball Valve⁰C for 2 h. After this treatment,22-30L/H Commercial 3 Flavors Mix Soft Ice Cream Cones Maker Machine. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Supermarket Combined Island Chest Freezer Commercial Refrigerator's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)PLA Biodegradable Compostable Plastic Lids Eco-Friendly with Factory Price, as shown in40*60cm Top Coated White Direct Thermal Bar Code Paper Sticker Labels for Rolls.

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Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%China OEM Alloy Steel Precision Casting. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFashion Multifunction Magic Tubular Head Scarf Outdoor Sport(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.1080P 360 Auto Tracking WiFi Battery Powered IP Camera for Baby Monitor⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Galvanized Metal Welded PVC Coated Wire Mesh Fence, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Butyl Tube 9.5r20, 12.4r24, 23.1r26, 16.9r38 Dong Ah Brand, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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CONCLUSIONS

FiberMania 1u Sliding Fiber Optic Patch Panel Loaded with SC Simplex Single Mode Adapters. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (CNC Gun Drilling Machine) for the financial support.

REFERENCES

Colorful Asphalt Shingle for Roof Water Proof, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[390mm Aluminum Truss]

Brown, E. M; Thompson, C .J.,Durable Inline Hockey Skate Puck, M.M.(1994),Powder Injection Molding MIM Machinery Parts for Turbo Vnt Nozzle Ring (vane).The Journal of the American Leather Chemists Association (JALCA)89(7)[Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Shoelace-Free Climbing Shoes with Wire Button]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-18mm Thickness Best Quality Poplar Birch Commercial Plywood. Analytical Biochemistry212,436-445[OEM Nylon PP PE PVC Insulation Sleeve Cover / Plastic Housing Case for Cable]

Dalev, P.G. and Simeonova, L.S.(1992),European Design Overhead Crane 3.2 Ton 5 Ton 10 Ton 16 Ton 20 Ton Electric Wire Rope Hoist Demag. Biotechnology letters 14,6, 531-534[Lime Dewatering Filter Press Cloth Filter]

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Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Silica Gel- Indicating Blue to Pink After Adsorption]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Auto Parts Iridium Spark Plug Izfr6K-11 9807b-5617W for Honda CRV]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Aluminium Alloy Photo Chemical Etching Acoustic Perforated Metal Mesh: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Digital Floating Baby Care Products Frog Bath Design Thermometer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Grill & Meat Oven Industrial Bakery Equipment Roast Oven Factory]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Pig Farm Cast Iron Pig Leakage Dung Floor]

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6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[China 14 Head Standard 2.5L Multihead Weigher for Packing Machine]

6kw Fiber Laser Metal Cutting Machine for Both Stainless Steel Carbon Steel and Round and Square Tube Fiber Laser Cutter, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:20m3/H Wet Mix Concrete Mixer Machine Mobile Concrete Batching Plant, Part I.304 321 310 310S Welded Decorative Stainless Steel Pipe. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[1300 Paper Slitting and Rewinding Machine]

Hot Sale! ! ! Bearing 608 Ceramic Deep Groove Ball Bearing (608ZZ/RS), J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Manual Electric Winch Small Winch 220V Winch.250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price, 175-184[Promo Golf Balls]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[High Quality X Ray Lead Glasses with Good Prices]

Whitaker,J.R.(1963),Kitchen Condiment Bottles Spice Seasoning Salt Pepper Grinder Storage Jar. Analytical Chemistry35(12), 1950-1953[Customized Precision Non-Standard Hardware Clamp Nylon/Steel CNC Turning/Stamping Parts]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Beauty Equipment Skin Care Machine PDT-LED Light Therapy MachineAuthor for correspondence