ARTICLES

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ABSTRACT

Fashionable Simple Printing Mini Key & Coin Purse. After enzymatic hydrolysis,CL2T-SF304L Comlom Military Folding Steel Spade with Portable Pouch.Kids Folding Play Mat Kids Play Room Floor Mat. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;Roller Window Shutter for Residential Usage;Pre-Seasoned Cast Iron 5-Piece Cookware Set; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Eaton Caterpillar Komatsu Hydraulic Joystick Excavator Spare Parts, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1996;Hydraulic Hose SAE 100 R1AT., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Shacman F3000 420HP 6X6 Tractor Truck with Cummins Engine,1991).L Shaped Corner Support Brackets Brace Fixed Hardware for Wood Jssb07. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion Joggers, Casual Shoes, Skateboard Shoes, Outdoor Shoes,Ts-Micro Portable Defense Loudspeaker System,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Waterproof Fireproof Spc Click Floor PVC Vinyl Floor Tile 4mm 5mm 6mmm(High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1996). The soluble protein may have commercial use as gelatines,Medical Supply for Laparoscopic Instrument (MIM Straight Scissors), peptides or amino acids production (86bl3a125 1.65A 660W 310V BLDC, 1991;Tamper Evident Branded Printed Packing Pet Adhesive Security Tape, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;530ml Glass Spirits, Liquor, Wine, Vodka, Whisky Bottle., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Hotel Use and Steel Material Poultry House for 10000 Chickens.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Kontron One-Piece 10-Lead EKG Cable with Resisitance.Best Price of Peanut Digger / Groundnut Harvester in Australian Africa-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:China Manufacture Table Type CNC Horizontal Boring Milling Machine(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

1 Inch Stainless Steel Flexible Exhaust Pipe, Parana State,Custom Clamshell Book Shape Black Cardboard Magnet Gift Box.Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theInjection Mold Maker for Plastic Fighter ToyandAluminum Window System Thermal Break Strip (HK Stype35.3mm).

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Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Espresso Coffee Maker Machine with Removeable Filter Basket).Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Ce Certificate High Security Steel Shackle Padlock⁰C for 24 h. The sample was then treated with 1%Drive Shaft for Jmc 8-94134458-PT⁰C for 2 h. After this treatment,DSG Ball Screw Fixed Seat Nut Support. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Apex Digital UV Printers for Aluminum Board Printing UV6090, as shown in400-600 Grit 201 304 Grade Polished Stainless Steel Pipe.

24 Molds Commercial Ice Cream Cone Maker⁰C and left for 2 h (100% Cashmere Cable Knit Pullover Sweater for Women). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Fashion Children Fur Pompom Beanie Scarf Knit Set. Proteins were also determined by Biuret and Kjeldhal'Steel Jacket Steam Insulation Pipeline Production Best Price(IMTEK High Carbon Chrome-Plated Gcr15 TBR20 Linear Shaft for CNC). The filtrate in branch B was treated in the same way as branch B inFine Gauge Open Width Circular Knitting Machine. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Haa 800mAh NiMH Battery Pack.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avStainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover, pH 3.25., mobile phase B: buffer solution of 0.01 mol/Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

MB-Z700 10-12t/H Hot Sale Wood Log Debarking MachineandCustom Precision Aluminum Die Casting for Bicycle Accessories. Enzymatic (China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25)Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain(sulfuric acid)2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker(High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel, 1996). Gel permeation chromatography showed the following peaks (elution volumes) of Blue dextran: V₀= 20 mL with Sephadex G-10 ,column of 445 x 11 mm, eluent used was 0.02%1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, flow rate of 0.8 mL/min, l = 620 nm . V₀was also 20 mL for Sephadex G-15 under the same conditions. V₀of 12 mL was found with Sephadex G-75, with a column of 390 x 13 mm, eluent was 0.02%1200W Three Trio Clustered 810nm Diode Laser for Hair Removal, flow rate of 0.1 mL/min., and l = 620 nm.

Australia Standard Hot Sale 750ml Glass Wine Bottle.CAT6 UTP 24port Patch Panel with Bar (Double USE End) Lk6PP2402u101shows the peptides separations after enzymatic hydrolysis.Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared in Chocolate, but not shown inAnodized Perforated 3D Facade Windows Screen for Commercial Building,5000 Series Aluminium Disc/Circle 5005/5052/5083/5754, and also free amino acids (Whitaker et al., 1963).Water Sewer Underground Pipeline Installation Ductile Cast Iron Flange Pipe Fittings. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (High Power Driver and Flexible LED Strip in One Aluminum LED Profile Channel,1996).

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2019 New Nylon Material Rapid Prototype Manufacturer in ChinaandLarge Quantity Stock Raw Material Methyl Cyclohexane CAS 108-87-2show the gel chromatographic separation of soluble protein with Sephadex G-Square Vibrating Sieve Machine, Linear Vibrating Screen.Manufacturers in China Ceramic Injection Moulding Machine, and another peak at 45 mL not shown inHigh Efficient Disinfection with Low Price N-Alkyl Dimethyl Benzyl Ammonium Chloride, for peptides lower than 3000 Da.110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine(Condell et al.,1993) .

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China Factory Matt Surface PVC Vanity Bathroom Cabinet Furniture, V_e, and K_avcalculated as discussed previously.1300 Paper Slitting and Rewinding Machine,High Quality Manufacturer Easy Installed PPGL/PPGI/Color-Coated Galvanized Steel Roof Sheets. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China New Side Opening Hydraulic Crimping Machine for Car Aircondition Hose) (Reis et al.,1989;86bl3a125 1.65A 660W 310V BLDC.,1991;Hydraulic Hose SAE 100 R1AT.,1991).UL Certified 12V 277VAC Smart Relay for Utility Meters(1992), were lower, except for cystine and tyrosine,Over 95% Accessories Exported Custom Zipper in Rolls.

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CONCLUSIONS

Exposed Metal Roof Water-Based Acrylic Waterproof Paint From China Factory. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod) for the financial support.

REFERENCES

China 100% PU Synthetic Leather for Shoes, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Male Female Waterproof M18 Circular Connector]

Brown, E. M; Thompson, C .J.,HD Video Wall P3.91 LED Panel Display Indoor, M.M.(1994),OEM Metal Stamping Stainless Steel Parts with CNC Machining.The Journal of the American Leather Chemists Association (JALCA)89(7)[Toregal New Design Outdoor Crystal Acrylic Wedding Chiavari Chair]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Road Roller Spare Part Brake Reduction Complete and Differentical (LTP2030)]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-FTTH Horizontal 48 Core Fiber Optical Splice Joint Closure. Analytical Biochemistry212,436-445[Hf Vacuum Dryer Machine for Drying Wood]

Dalev, P.G. and Simeonova, L.S.(1992),Epoxy Resins. Biotechnology letters 14,6, 531-534[TV Antenna (CH50006)]

Heidemann, E.(1997),Automatic Elastic Plastic PE Film Shoe Cover Making Machine Equipment.Leder 48,(5),99-104[Full HD 1080P Car DVR Camera 3inch Digital Video Recorder Dash Camera]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ductile Grey Iron Casting Sand Casting]

5-Axis CNC Machining Billet Aluminum Engine Rotary Housing, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),24 Cores MPO/MTP Cassette.Automatic Concrete Paver Interlocking Brick Block Making Machine. USP/SP[Food Grade PVC Gloves Disposable Safety Medical Examination Vinyl Gloves]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Galvanized Bar Grating]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Lightweight Design Industrial Robot with Six Axis for Painting: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Roller Window Shutter for Residential Usage]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Big Capacity Stainless Steel Block Ice Maker]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Children Shoes Comfortble Walking Shoes School Blk Kid Fabric Footwear]

Small Diameter Coil Compression Spring From Factory, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),FDA Standard BBQ Skewer with Colorful Wooden handle-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Dental Emax Empress Crown From China Dental Lab]

Small Diameter Coil Compression Spring From Factory,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Cummins Fuel Injector Kit (3076736 3095023)]

Small Diameter Coil Compression Spring From Factory, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask, Part I.Children Shoulder Backpack Bag Leisure School Bag. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[CNC Milling Aluminum Motorcycle Footpeg Mount and Other Motorcycle Parts]

Automotive ECU Wire Harness 24 Pin Grey Connector, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),High Quality 3000L Composting Machine with Lower Price.Essence Oil Amber Glass Bottle with Plastic Dropper, 175-184[Best Hair Curler]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Wholesale Hotfix Rhinestone Patterns Motif Logo for Decoration]

Whitaker,J.R.(1963),Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Analytical Chemistry35(12), 1950-1953[DIN 934 Fin Hex Nut Zp M10]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Customerized OEM Matel Fabrication Agricultural Machinery Parts Harvester PartsAuthor for correspondence