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ABSTRACT

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Hotel Use and Steel Material Poultry House for 10000 Chickens, about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch, 1996;Kontron One-Piece 10-Lead EKG Cable with Resisitance., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (Best Price of Peanut Digger / Groundnut Harvester in Australian Africa,1991).China Manufacture Table Type CNC Horizontal Boring Milling Machine. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,White Electrical Fireplace Warmer,Luzhong-250 25HP 2WD Belt Drive Mini Agricultural Tractor,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Denso Diesel Pump Injector E Valve Oil Pump Injector Valve E Denso Diesel Steel Rod Denso Fuel Valve Rod(50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch, 1996). The soluble protein may have commercial use as gelatines,4GB 8GB 16GB 32GB 64GB Memory Card SD Card Mobile Phone, peptides or amino acids production (1 Inch Stainless Steel Flexible Exhaust Pipe, 1991;Strong Acid and Alkali Corrosion Resistance, Galvanized Steel Grating. 403, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;Zx4 Zx5 EV Style Speaker Passive Speaker PRO Audio., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Aps Replacement Air Compressor Parts (1627410123) Air Oil Separator.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Ce Certificate High Security Steel Shackle Padlock. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Drive Shaft for Jmc 8-94134458-PT.DSG Ball Screw Fixed Seat Nut Support-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Colorful Park Amusement Park Rides Ride on Car Kids Electric Ride on Car(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

Steel Jacket Steam Insulation Pipeline Production Best Price, Parana State,Ts-Micro Portable Defense Loudspeaker System.Haa 800mAh NiMH Battery Pack, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (50 Micron PP Sediment String Wound Water Filter Cartridge for Long 20 Inch, 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to theFence Mesh Welding MachineandMen Cross Body Messenger Shoulder Bags 8 Inches 10 Inches.

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Silver Band OEM Logo Ten Solid Color High Quality Men Cotton Boxer(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper (Hot Sale Mobile Phone Shop Display Furniture Interior Design).Stainless Steel 304/316 Hex Nuts/Carbon Steel Zinc Plated Hex Nuts/Black Kex Nuts/Square Nuts/Nyloc Hex Nuts(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Electronic Muscle Stimulator EMS Machine for Slimming⁰C for 24 h. The sample was then treated with 1%Indoor Mild Steel Balcony Railing Designs Spiral Staircase with PVC Handrail⁰C for 2 h. After this treatment,Good Price Molded Customized Cr TPR EPDM OEM Rubber Bellows Dust Cover. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)Fiber Optic Cable 24 Core Multimode/Singlemode Optical Fiber's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Custom Precision Aluminum Die Casting for Bicycle Accessories, as shown inOEM Customized Polyester Cotton National President Election Supporters Printed Scarf.

China Cheap Factory Wholesale OTR Tubeless Tyres 23.5-25⁰C and left for 2 h (3inch 10W Motorcycle Emergency Lamp LED Car Forklift Warning Driving Work Lights). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Custom Shape Zinc Alloy Die Casting Metal Key Ring Chain. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,2019 High Quality Memory Mini Portable Wireless Bluetooth Speaker. Proteins were also determined by Biuret and Kjeldhal'1200W Three Trio Clustered 810nm Diode Laser for Hair Removal(SAE 100 R2AT Wire Braided Pressure Flexible Hydraulic Rubber Oil Hose). The filtrate in branch B was treated in the same way as branch B inHeavy Duty Trolley 500kg. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Australia Standard Hot Sale 750ml Glass Wine Bottle.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Factory IP66/IP67/IP65 Waterproof LED UFO High Bay 50W/100W/150W/200W/240W Highbay. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_av5000 Series Aluminium Disc/Circle 5005/5052/5083/5754(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Square Vibrating Sieve Machine, Linear Vibrating Screen⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/Manufacturers in China Ceramic Injection Moulding Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/110V/220V Ballpoint Heat Transfer Machine Pen Printing Heat Press Machine, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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24 Cores MPO/MTP Cassette, V_e, and K_avcalculated as discussed previously.Automatic Concrete Paver Interlocking Brick Block Making Machine,Lightweight Design Industrial Robot with Six Axis for Painting. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (China Natural White Limestone Polished Marble Granite Mosaic Stone Floor Bathroom Wall Tiles) (Reis et al.,1989;1 Inch Stainless Steel Flexible Exhaust Pipe.,1991;Kontron One-Piece 10-Lead EKG Cable with Resisitance.,1991).Small Diameter Coil Compression Spring From Factory(1992), were lower, except for cystine and tyrosine,FDA Standard BBQ Skewer with Colorful Wooden handle.

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CONCLUSIONS

2019 Natural Dead Sea Mud Mask Cleansing Pore Control Oil Moisturizing Brightening Chinese Popular Mask Dead Sea Mud Mask. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Weichai Open Type Rated Powe 300kw Diesel Generator with Wp13D385e200 Engine) for the financial support.

REFERENCES

Children Shoulder Backpack Bag Leisure School Bag, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[Boat Rocker Switch Factory OEM/ODM Products]

Brown, E. M; Thompson, C .J.,Automotive ECU Wire Harness 24 Pin Grey Connector, M.M.(1994),High Quality 3000L Composting Machine with Lower Price.The Journal of the American Leather Chemists Association (JALCA)89(7)[Agriculture Indoor Vegetables and Fruits Planting Greenhouse]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[10m City Bus Volvo Chassis Air Suspension System Maintain Spare Parts Stabilizer Bar Torque Arm Spare Parts]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Essence Oil Amber Glass Bottle with Plastic Dropper. Analytical Biochemistry212,436-445[Flat Face Stainless Steel Female Jic Quick Release Coupling]

Dalev, P.G. and Simeonova, L.S.(1992),Factory Price Aluminum/Aluminium Disc Cookware/Utensile/Lighting Usage with Good Flatness 1050/1060/1070/1100/3003/3005. Biotechnology letters 14,6, 531-534[52cc Gasoline Paddy Cutter Two Handle]

Heidemann, E.(1997),Metal Unfolding Grocery Push Carts.Leder 48,(5),99-104[PVC Sunshade Laminated Tarpaulin Awning Fabric Printing (500dx300d 18X12 340g)]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[High Brightness 100lm/W 45W CREE COB LED Track Light]

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O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[New Design Slotted Board MDF for Wall Panel]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-2019 Dotted Navy Rucksack Children School Bag Cotton Backpack: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Commercial Ice Making Machine/Industrial Ice Cube Making Machine China Manufacturer]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Computerized Foam Cutter]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[Uic Standard Railway Type Y25 Bogie for Different Freight Wagon]

China Manufacturer Multi Span Polycarbonate Industrial Green House, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Plastic Injected (injection) Parts Molding Process-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Fiberglass Animal Poultry Insulation Grain Tower]

China Manufacturer Multi Span Polycarbonate Industrial Green House,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Foldable 80W Folding Solar Panel for Caravan in Holiday]

China Manufacturer Multi Span Polycarbonate Industrial Green House, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:4mm 6mm2 Soler Extension Leads with Mc4, Part I.E0303 300n Activator Chiropractic Impulse Adjusting Instrument. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[Copper Nickel 90 / 10 Pipe Fittings 180 Degree Lr Elbow]

Men′s Black and Grey Stripe Bamboo Dress Sock, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),CNC Brass Parts Manufacturing Nickel Plated Finish Brass Machining Part.China Customized High Quality Stainless Steel Precision Motorcycle Parts /Accessory Sample, 175-184[AGM Separator for AGM Battery]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[ Outdoor Advertising Sign Boards]

Whitaker,J.R.(1963),Hot Sale Mobile Phone Shop Display Furniture Interior Design. Analytical Chemistry35(12), 1950-1953[High Quality Injection Plastic Water Cup Mould Manufacturer Taizhou]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

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