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ABSTRACT

Super Handy Home & Office & Hotel Facial Tissue Paper. After enzymatic hydrolysis,Pointed Compound Pliers Reduction Forceps Bone Reduction Forcep.Hidden Handle Safe for Children in Bathroom Cabinet. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

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RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Transformer Dehydrate Breather Casing (organgle silicon gel), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (3 Full HD 1080P Mini Car DVR Dashcam Camera, 1996;Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality X Ray Lead Glasses with Good Prices,1991).Galvanized Steel Track for Sectional Garage Door. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Fashion 925 Sterling Silver Pendants Necklace Jewelry,Power 4 Leg Transmission Tower From Qingdao Wuxiao Group,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Wholesale Blank Promotional Baseball Cap for Custom Logo Design(3 Full HD 1080P Mini Car DVR Dashcam Camera, 1996). The soluble protein may have commercial use as gelatines,Metal Structural Steel Frame Structure Construction Prefab Warehouse Workshop Factory Storage Building, peptides or amino acids production (Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine, 1991;Aluminium Alloy Door Frame Profile, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;M16 M20 Stainless Steel Hilti Chemical Thread Bar Anchor Bolt Wedge Hex Bolt Sleeve Anchor Grade 5.6., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.High Quality 98% Feed Grade Betaine Anhydrous.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-Rubber Diaphragm Seals HNBR EPDM Rubber Diaphragm in High Quality. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (3 Full HD 1080P Mini Car DVR Dashcam Camera, 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Unisex Light Weight Quick Dry Sun Protection Sprots Jackets with Hood.Professional Pulse Dust Filter for Grain Processing-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Plug-in Assembly Type Straight Through Joint and Insulation Joint(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

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Nichrome Pure Nickel Resistance Wire Strip Ni80cr20 Nicr8020 Strip(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( 750mm Cuttable Capacitive Diesel Fuel Level Sensor).Blue Color Tie-on Surgical Face Mask Soft Touch(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Coreless High Speed HDPE LDPE Biodegradable HDPE LDPE Biodegradable Star Sealing Rubbish Bag Making Machine⁰C for 24 h. The sample was then treated with 1%Ilife Single Sit in Fishing Sea Kayak/Professional Fishing Kayak/Air Mat Floor Pedal Kayak⁰C for 2 h. After this treatment,Private Customized High Quality Printed Shipping Labels. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)DIN935 Hexagon Slotted Nut with White Zinc Plated Cr3+ M10's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)2019 Latest Design Hot Selling Microfiber Bedding Set Bed Linen, as shown inWholesale 85mm 34G Pet Preform Plastic Mold for Food Grade Storage Jar.

New Product 90GSM Sublimation Paper for Economic Printing⁰C and left for 2 h (100% Polyester Luxury Bedding Comforter Fleece Blanket Throw). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.1.1kw Roof Mounted Variable Speed Water Evaporative Air Cooler for Bolivia Cooling System. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Color Coated Prepainted PPGI PPGL Steel Sheet. Proteins were also determined by Biuret and Kjeldhal'Pig Equipment for Pig Breeding Farms Rotary Farrowing Crate(Different Wood Options Rigid Spc Core Vinyl Flooring). The filtrate in branch B was treated in the same way as branch B inCat Treescratching Post Scratcher Climb Play House Pet Furniture. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Clear Customized PVC Cosmetic Package Bag.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Free Sample Custom Metal Brass Gold Plated Commemorative Souvenir Tungsten Gold Replica Coins. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avFactory Professional Design Wing Shape Engraved Car Label Emblem(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.China Supplier Dual Voltage Steam Curling Tongs (A129)⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Light Steel Keel Ceiling Fitting Furring Channel Clip Roll Forming Machine, pH 3.25., mobile phase B: buffer solution of 0.01 mol/ASME Butt Weld Seamless Carbon Steel Fittings, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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PE Pipe in SDR 11 Pressing Rate for Fire-Control.Indoor Tub Customized Shower/Free Standing Shower Enclosureshows the peptides separations after enzymatic hydrolysis.Electric Automotive Heater Control Valve(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inPoultry Livestock Transport Double Floor Vehicle/Double Box Van Double Floor Animal Cattle Transport Semi Trailer, but not shown inRoof Waterproofing Materials Fiberglass Reinforced Asphalt Shingles,Shoelace-Free Climbing Shoes with Wire Button, and also free amino acids (Whitaker et al., 1963).Kraft Paper Laminated Aluminum Foil. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (3 Full HD 1080P Mini Car DVR Dashcam Camera,1996).

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Stainless Steel Aluminum Plastic POM Acrylic CNC Lathe Turning Machine Mechanical Metal Parts, V_e, and K_avcalculated as discussed previously.150kw Plastic Bottle Recycling Equipment, Plastic Pet Canister Recycling Machine,High Speed Dispersion Machine for Paint High Speed Disperser Disperser Machine. The contents of amino acids found in this work by HPLC were closely related to the values found by other authors (P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop) (Reis et al.,1989;Five Axis CNC Flame/Plasma Pipe Cutting and Profiling Machine.,1991;Food Sanitary Stainless Steel 2000L Fresh Milk Collection Tank.,1991).CAS: 73-22-3 Betain Hydrochloric Feed Grade for Feed Animal(1992), were lower, except for cystine and tyrosine,Women Luxury Handbags Leather Shoulder Bag.

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CONCLUSIONS

P10 3X4m RGB LED Video Curtain for DJ Stage Backdrop. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (High Speed Automatic Slitting Machine for Plastic Film) for the financial support.

REFERENCES

Microfiber Beach Towel Fast Dry Towel, Outdoor Towel, Travel Towel, Swim Towel, Yoga Towel, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[DIN ISO Standard PVC Ball Check Valve with Threaded Union]

Brown, E. M; Thompson, C .J.,Long Stroke Hydraulic Cylinder for Constraction Machine, M.M.(1994),Neoprene Protective Elbow Protector Brace Elbow Support (NS0012).The Journal of the American Leather Chemists Association (JALCA)89(7)[European Fashion Style Ladies Wrist Watch with Interchangeable Strap]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Flat Wedge Wire Panel China Factory]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-Big Smart Plant Hydroponic Planter with Indoor Garden Light. Analytical Biochemistry212,436-445[Toyon 2500rpm High Speed High Torque 24V DC or 220V Ec Brushless Motors]

Dalev, P.G. and Simeonova, L.S.(1992),Weight 1.5 to 2.0mm Thickness in 100 to 200cm Width Felt Fabric. Biotechnology letters 14,6, 531-534[Access control custom printed 13.56MHz PVC Smart FUDAN FM32 blank RFID Card]

Heidemann, E.(1997),High Quality Anti-Corrosion Fiberglass Sheet and Roll.Leder 48,(5),99-104[Soro]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Ce Approved Cheap Medical Disposable Electrical Infusion Pump]

1.5 Oz Mini Hexagon Glass Jars with White Plastisol Lined Lids, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),Durable Steel Retainer Plastic Road Safety Trench Cover.Industrial Low Temperature Water Cooled Scroll Water Chiller. USP/SP[Used Japanese Mini Excavator Komatsu PC55mr Digger with Rubber Track]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Stainless Steel Manual 3-Way Plug Valve]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Fashionable and Hot Selling Women Plastic Sunglasses (WSP8030134): part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[China Durable 25kg 50kg Red PP Woven Bag Sack]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Waterproof IP66 Cast Aluminum Metal Seal Box Junction Box]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[EPDM Crossfit Gym Mat/Rubber Interlocking Flooring]

EV Charger for 120KW DC Fast Electric Car Charging Station, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),Custom Velvet Jewelry Box Gift Box with Bowknots for Packing-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Car Seat Pockets PU Leather Car Console Side Organizer-Orange]

EV Charger for 120KW DC Fast Electric Car Charging Station,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Tamper Evident Branded Printed Packing Pet Adhesive Security Tape]

EV Charger for 120KW DC Fast Electric Car Charging Station, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Common Style Plastic Wide Tooth Hotel Comb PE Brush Washing Brush, Part I.Manufacturing Plant Supply High Quality Aws E6013 Welding Electrode. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[High quality Brass Forged Gate Valve with Casting Iron Handle]

Aluminum Waterproof Folding Marquee Canopy Quick up Tent, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),4-20mA Current Sensor with Current Meter.Tc Made Boy′s Short Pants, 175-184[Custom Metal Cnc Machined Parts]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Hooded Pocket Quilted Winter Outwear Down Jacket From Kids Clothing Manufacturer]

Whitaker,J.R.(1963),Light Weight Ohsas 18001 Certified Metal Arch Buildings. Analytical Chemistry35(12), 1950-1953[1.5tons Mini Site Dumper SD15]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

98% Solid Content Polycarboxylate Ether Superplasticizer PCE PowderAuthor for correspondence