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ABSTRACT

Single Block Vertical Wire Drawing Machine for Screw and Bolt Making. After enzymatic hydrolysis,High Chrome Hsi Crusher Parts Blow Bar Suit Terex Impact Crusher.China Electric PCB Circuit Board with High Quality. Glycine (17%) , glutamic acid (10.6%) , alanine (9.2%) , and arginine (8.2%) were the principal amino acids found. Phenylalanine(1.8%) was the main aromatic amino acid , while tryptophane was completely absent.

Key words;OEM Custom Forged Auto Parts, Precise Lost Wax Die Casting and CNC Machined Alloy /Aluminum/Zinc /Brass Forklift Truck Parts;Anodized Perforated 3D Facade Windows Screen for Commercial Building; chromatography; protein, chromium

RESUMO

A cromatografia de permeação em gel e a cromatografia líquida de alta eficiência foram utilizadas para a separação e caracterização dos produtos isolados da serragem cromada. Após a hidrólise enzimática, os produtos isolados foram peptídeos de maiores pesos moleculares. Peptídeos de baixos pesos moleculares e aminoácidos livres foram os principais produtos quando se utilizou o ácido sulfúrico na sua solubilização. Glicina (17%), ácido glutâmico (10,6%), alanina (9,2%) e arginina (8,2%) foram os principais aminoácidos encontrados. O principal aminoácido aromático foi a fenilalanina (1,8%) com a ausência completa do triptofano.

INTRODUCTION

Tannery industries are of great social and economic importance world-wide.Full Cream Milk Powder Filling Machine (XFF-G), about 600 industries produce approximately 22 million leather per year, approximately 10% of total world production (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., 1996;Dnv 271 En 12079 3.5 Cbm Offshore Mud Skip., 1996). Tanneries use different types of processes to tan the leather, and tanning with chromium salts are of great environmental concern because of its by-products (High Quality Wc67K 40 Ton 80 125t 2500 2.5m Metal Sheet Plate Construction Industry Hydraulic Small Mini CNC Bending Press Brake Machine Factory Price for Sale,1991).Digital Printed 3D Carpet, Anti-Slip Hot Sale Floor Mat. Various alkalinity-inducing agents such as magnesium oxide, calcium hydroxide,Large Water Capacity PP Pleated Material 60 Inch 5 Micron Water Cartridge Filter for Power Plant Treatment,PVC Car Seat Belt Shoulder Protector 80438,etc.,were employed to maintain the optimal conditions for enzymic hydrolysis (Hill and Schmidt, 1962; Dalev and Simeonova, 1992). Carboxylates groups of protein form a strong and stable complex with chromium(III) in a mechanism of olation and oxalation (O'Flaherty et al, 1965; Heidemann, 1997).

Due to this stable complex, the complete removal of chromium(III) from the soluble protein is a very difficult task,Twin Screw Floating and Sinking Fish Shrimp Food Feed Making Extruder(Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., 1996). The soluble protein may have commercial use as gelatines,Professional Full Automatic Machine for Making Wood Pellets, peptides or amino acids production (High Quality 20PCS Colorful Makeup Brush Set, 1991;Big size 420mm*420mm*460mm building plate,printing Rehabilitation of protective, 3D Printer used in rehabilitation therapy, 1992; Brown et al., 1994; Ta ylor et al., 1998). Chromium recovered after washing may be recycled by tanning industry or used in other industrial applications (Collivgnarelli and Barducci, 1991;PE Plastic Road Safety Barricade Caution Tape., 1998).

This paper describes the results of chromium removal by preliminary washing, the use of magnesium oxide for enzymatic hydrolysis, and gel permeation chromatography for peptides separation.Screw Barrel for PVC PPR Pipe Extrusion.

MATERIALS AND METHODS

Apparatus

Atomic absorption spectrometer CG-AA-7000-ABC (AAS), with an air-15kgs/Spool Aws A5.18 CO2 Gas-Shielded MIG Welding Wire Er70s-6. UV-VIS-Beckman DU-70 spectrophotometer was used for peptides determination (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., 1996). Thermostatic bath, Tecnal TE-184, temperature range of 0 to 99⁰C ± 1⁰Gold Open Metal Short Crocodile Wallet for Girl Women Lady.6kVA Single Phase High Frequency Online UPS with 0.8/0.9 Power Factor-LDC-Analytical (Pickering Laboratories, Inc.) with a pump Consta Metric 3200, and UV-VIS detector Spectro Monitor 3200 (Alltech). The following columns were also used:Pond Self Priming Booster suction  Water Pump with Pressure Sensor(8µm, 3 x 200 mm), (Pickering Laboratories, Inc.), and a pre-column (8 µm, 2 x 200 mm).

Reagents

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Sample collection and preparation

European Standards 100A 24V Electrical Relay for Green Energy, Parana State,Antique Stackable Metal Tiffany Hotel Event Hall Chiavari Banquet Church Chair.Best Quality Snow Beach Mountain Motorized Electric Bicycles with Pedal, exposing directly to the sun during 5 or 6 h. The preliminary washing step was performed with 2% (m/v) solution of oxalic acid in thermostatic bath at 50⁰C and 2 h with shaking (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards., 1996). After removal of chromium, the leather solubilization by chemical and enzymatic methods were performed according to the3D Creative Design Metal Medal Promotion Gift of Sportand250ml Medical Transparent Ultrasound Ultrasonic ECG Gel Wholesale Price.

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Canvas Shoulder Weekend Shopping Big Bag(2.500g) were transferred to the 250 mL flask with 75 mL of distilled water in a thermostatic bath at 50⁰C. After 2 h, the solid was filtered using qualitative filter paper ( Powder).High Visuable Reflective Tape for Vehicle Road Safety(branch B) until pH 10, in order to precipitate Cr(OH)₃. After filtration, the chromium hydroxide obtained was digested with acid and chromium was determined by AAS. The solid residue (branch A) was treated with 3% magnesium oxide (w/v) and 10%Made in China Aluminum Extrusion Window and Door Profile⁰C for 24 h. The sample was then treated with 1%Industrial Mini PC, Support Intel Core3 I7/I5/I3 Processors, Gigabit Ethernet and WiFi. Embedded Ipc⁰C for 2 h. After this treatment,Seaflo 12V 0.7gpm/70psi Mini Electric Garden Pump. The filtrate was separated in three portions for the following experiments: a) protein determination by Biuret's method (Smith,1985); b)304/304L 2b Stainless Steel Sheet and Steel Plate's method (Standard Methods for the Examination of Water and Wastewater, 1992); c)Multi-Function WiFi Control Table Laser Cutting Engraving Machine Supplier, as shown inGalvanized Iron / Stainless Steel Crimped Square Woven Wire Mesh.

Plastic Injection for OEM Product⁰C and left for 2 h (Cooler Pad Wet Corrugated Paper Water Curtain Evaporative Cooling Pad for Poultry Farm). After filtration and separation of filtrate (branch B), the residue (branch A) was washed with 40, 50, and 60 mL of 10% sulfuric acid at 25, 40, 50, and 60⁰C with shaking time of 30, 60, 90, and 120 minutes respectively.Cable Railng Spiral Staircase with PVC Handrail for Interior Space Saving. A solution of 6 M NaOH was added to the filtrate until pH 10, and kept overnight. After filtration, the solid Cr(OH)₃was kept at 600⁰C for 2 h,Durable Anti Slip Tennis Court Sport Rubber Flooring/Outdoor Court Material. Proteins were also determined by Biuret and Kjeldhal'Bison (China) BS2500u (E) 2kw 2kVA Ce Certificated AC Single Phase Factory Price Continent Name Generator(Powerful Neodymium Pot Magnet Rare Earth Pot Magnet). The filtrate in branch B was treated in the same way as branch B inNylon Hammer drive Anchor with Zinc. The final soluble protein shown were used for peptides separations using gel permeation chromatography,Experienced Construction Steel Structure Building Design.

Chromatographic separation

Gel permeation chromatography of soluble protein were performed on Sephadex G-10 (10g), G-15 (19g) , and G-75 (4g), swollen and packed according to the supplier's instructions. An aliquot of 1.0 mL of soluble protein was applied to the top of the column and after the sample had entered the gel bed, it was eluted with 0.02%Heavy Duty 16000L High Pressure Sewer Cleaning Truck. Absorbance at 235 and 280 nm were measured using 1 cm quartz cubets. The void volume (V₀) was determined from the elution of 0.4% solution of Blue Dextran, reading the absorbance at 620 nm.

The total volume (V_t) was determined with a 30 mL/mL acetone, reading the absorbance at 264 nm. Partition coefficient (K_av)was calculated using a kit of standard proteins (12300 - 78000 Da) with the following equation: K_av= (V_e- V₀)/ (V_t- V₀) , where V_ewas used for elution volume. Plot of K_avWinter Design Acrylic Beanie /Bobble Jacquard Hat with Flowers /Machine for Knitted Winter Cap with Embroidery Circular Hat Knitting Machine(Daltons) of 16949 (myoglobin), 30000 (carbonic anhydrase), 42700 (ovalbumin), 66250 (albumin), and 78000 (ovotransferrin).

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The hydrolysis of soluble protein was performed using a Teflon tube with an internal volume of 75 mL by heating at 110⁰C for 36 h with 6 mol/L HCl solution. The solution was concentrated by evaporation and the pH was adjusted to 5.0 - 5.5.Portable Ink Date Printing Machine for Plastic Bag⁰C (flow rate 0.3 mL/min.). Ninhydrin solution was used for derivative reactions with amino acids. The eluents used were: mobile phase A: buffer solution of 0.2 mol/High Quality Professional HD IPS 10.1 LCD Monitor with SDI, 4K HDMI2.0, pH 3.25., mobile phase B: buffer solution of 0.01 mol/High Frequency 500W Pure Sine Wave Inverter Circuit Board for Power Inverter 50Hz/60Hz, pH 7.40. Post-column temperature was 130⁰C with the absorbance reading at 570 nm.

RESULTS AND DISCUSSION

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Dual Frequency RFID Key Cards for Both Hotel Elevator & Room.3 Layer Film Blowing Machineshows the peptides separations after enzymatic hydrolysis.NSF 5 Layers Powder Coated White Kitchen Bathroom Corner Storage Wire Shelf Unit Rack(Tayyab et al.,1991). The volume (peak) of 35 mL that appeared inAluminum Plate Fin Heat Exchanger/Oil Cooler Core, but not shown inChina Original Dongfeng Cummins 6CT Diesel Engine Fuel Transfer Pump,Most Effective Elight+IPL+RF+ND YAG Laser Dermatology Instrument, and also free amino acids (Whitaker et al., 1963).Hot-Sale Products GRP Tower for New Energy Industry. The same results were observed using Biuret and Kjeldhal's methods for peptides analyses (Handmade Paper 3D Quilling Greeting Cards Quilling Birthday Cards.,1996).

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CONCLUSIONS

Quartz Liquid Powder Feeding Torch. Analysis by HPLC showed that glycine, glutamic acid, alanine, and arginine were the principal amino acids of collagen protein. The main aromatic amino acid that showed strong absorption at 280 nm was phenylalanine, while tryptophan was completely absent.

ACKNOWLEDGMENT

The authors would like to thank FNMA/MMA , CAPES, and CNPq (Grass Rubber Matting/Outdoor Rubber Mats/Oil Resistance Rubber Mat/Ship Deck Rubber Mats) for the financial support.

REFERENCES

Top Stainless Steel Baluster Designs Stair Baluster Railing Handrail, A.R.E.; Van der Poel, A.F.B.; Koene, J.J.A. & Dieleman, S.H.(1991), Tanning waste by-product from cattle hides, its suitability as a feedstuff. Bioresource Technology35,321-323[75 Mold Pe Fruit Net Machine]

Brown, E. M; Thompson, C .J.,Medium Sleeve Length Waterproof Pet Shower Clothing Dog Cat Grooming Garment Clothing, M.M.(1994),Outdoor P5mm Programmable Bus LED Display Sign.The Journal of the American Leather Chemists Association (JALCA)89(7)[PSF 1000 Flat Plate Upper Discharge separator]

Collivgnarelli, C. and Barducci, G.(1984), Waste recovery from the tanning industry. Waste Management & Research2, 265-278[Essence Oil Amber Glass Bottle with Plastic Dropper]

Condell, R.A., Hanko,V.P., Larenas, E.A., Wallace,G.,and McCullough,K.A(1993), Analysis of native collagen monomers and oligomers by size-exclusion high-AGM Separator for AGM Battery. Analytical Biochemistry212,436-445[Suction Filter Cylinder Match 48 Series Filter Brands]

Dalev, P.G. and Simeonova, L.S.(1992),Electric Battery Flameless LED Tea Light. Biotechnology letters 14,6, 531-534[Reverse Osmosis Water Filtration Device/RO Plant Process/RO Plant Price]

Heidemann, E.(1997),42/57/60/86/110mm 24V 36V 48V 60V BLDC Brushless DC Electric Motor.Leder 48,(5),99-104[aihdcam.com]

Hill,R.L. and Schmidt,W.R.(1962), The complete Enzymic Hydrolysis of Proteins.The Journal of Biological Chemistry 237, 2, 389-396[Straight Seam Galvanized Pipe High Frequency Tube Welding Mill]

Ribbon Mixer Plough Shear Mixer V-Shape Mixer Powder Mixer for Mixing Powder or Liquids, C.C.M.; Garcia, E.E.; Martins, A.C. and Nozaki, J. (1996),CE Certificated Wheel Loader (HQ912) with Agricultural Tire.Commercial Vegetable Drying Equipment, Yam, Apple Drier with Trays. USP/SP[Wholesale Price Auto Iridium Spark Plug for Turbo Trumpchi Cars]

O'Flaherty, F.; Roddy,W.T. and Lollar,R.M. Editors (1965), The Chemistry and Technology of Leather,vol.I-IV,Reinhold Publishing Corp., New York, pp.1956[Hot Sale Effective Scc628 Water-Based Green Cutting Fluid From China Supplier]

Reis, M. and Beleza, V.(1989), Utilization of leather waste-Cosmetic & Personal Care Grade Xanthan Gum: part 1,pilot plant study. Journal of the Society of Leather Technologists and Chemistsv75,18[Disposable Pipette Tip 40~200UL with Ce]

Smith, P.K.(1985), Colorimetric method for the determination of protein. Anal. Biochem.,150,76-78[Cellphone Jammers]

Standard Methods for the Examination of Water and Wastewater(1992), 18^thEdition.Greenberg, A.E., Clesceri, L.S., and Eaton, A.D. Editors, Washington[5 Axis CNC Machining Part Clear Anodize High Precision 3D Horizontal Scanner Extension Adjusting Nut]

Professiona Ozone Generator for Swimming Pool, M.M.; Diefendorf, E. J. and Marmer,W.N.(1991),High Precision Plastic Mold Manufacturer in China-inducing agents. The Journal of the American Leather Chemists Association ( JALCA )86,5, 199-208[Toyota Electric Plastic Male Wire Harness Assembly Automotive Housing Connector]

Professiona Ozone Generator for Swimming Pool,M.M.; Diefendorf, E.J.; Thompson, C.J.; Brown, E.M., and Marver, W.N.(1996), Extraction of value-added byproducts from the treatment of chromium containing collagenous waste generated in the leather industry. AQEIC Bol. Tec., (3),124-150[Pre Engineering Building Prefabricated Building Material Steel Structure Building Warehouse Hangar Workshop with Construction Design]

Professiona Ozone Generator for Swimming Pool, M.; Cabeza, L.F.; DiMaio, G.;Brown, E.M.;Marmer, W.N.; Carrio,R.; Celma, P.J., and Cot,J.(1998), Processing of leather waste:Fiberglass Animal Poultry Insulation Grain Tower, Part I.Low Volume & Large Variety High Precision Stainless Steel CNC Machined Parts. The Journal of the American Leather Chemists Association (JALCA)93,(3),61[UV Resistant Single Component Water Based Polyurethane Waterproof Coating]

New M4 Smart Bracelet Waterproof Bluetooth Step Bracelet Information Call Reminder Heart Rate Bracelet Factory Direct Gift Watches Smart Watch, J.; Dihua, Mo; Yiwan, C. and Chumping, Z.(1992),Factory Black Polyformaldehyde Rod POM Sheet/POM Sheet/POM Rod/Acetal Rod/Delrin Sheet/Delrin Rod/Blue Delrin Sheet/Color Delrin Sheet/Colored Delrin Rod.Vp-0500 Intelligent Electric Valve Actuator Rotary Valve Motorized Actuator Low Torque Actuator for Ball Valve Butterfly Valve Quarter Turn Electric Actuator, 175-184[Automatic EPS Food Box Thermoforming Machine]

Tayyab, S.,Qamar,S., and Islam, M.(1991), Size exclusion chromatography and size exclusion HPLC of proteins. Biochemical Education19(3), 149-152[Non-Woven Medical Outside Earloop Mask Making Machine]

Whitaker,J.R.(1963),DIN 934 Fin Hex Nut Zp M10. Analytical Chemistry35(12), 1950-1953[TV Stand Carving European LED Lights Heating Fireplace (320SS)]

Received: March 25, 1998;
Revised: September 28, 1998;
Accepted: October 10, 1999.

Professional Full Automatic Machine for Making Wood PelletsAuthor for correspondence